| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 161,367 | A→G | 18.6% | N27S (AAC→AGC) | cdaR → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 161,367 | 0 | A | G | 18.6% | 250.1 / 52.0 | 113 | N27S (AAC→AGC) | cdaR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (37/55); new base G (9/12); total (46/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
CATCTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACGAAG > minE/161300‑161435 | cATCTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGt < 1:927988/71‑1 (MQ=255)cATCTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGt < 1:3816119/71‑1 (MQ=255)cATCTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGt < 1:2868069/71‑1 (MQ=255)cATCTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGt < 1:1427878/71‑1 (MQ=255)cATCTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATAATCGATACCAATATCAGCg > 1:1970903/1‑70 (MQ=255) tCTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGt < 1:1002488/69‑1 (MQ=255) tCTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGt < 1:1771290/69‑1 (MQ=255) tCTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTaa > 1:1125028/1‑71 (MQ=255) ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATg > 1:779070/1‑71 (MQ=255) ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATg > 1:2744403/1‑71 (MQ=255) ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATg > 1:3336296/1‑71 (MQ=255) gATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATgg < 1:3524767/70‑1 (MQ=255) gATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTaa < 1:2417553/67‑1 (MQ=255) gATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTaa < 1:2606939/67‑1 (MQ=255) gATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAAt < 1:3392474/68‑1 (MQ=255) gATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAAt < 1:886312/68‑1 (MQ=255) gATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATg < 1:3644164/69‑1 (MQ=255) aTACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGt < 1:2967328/64‑1 (MQ=255) aTACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGAt < 1:2733928/71‑1 (MQ=255) aCCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGc > 1:3236439/1‑71 (MQ=255) aCCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGc > 1:1731831/1‑71 (MQ=255) cAAAATGGCTCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGccc < 1:1828694/71‑1 (MQ=255) aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCg < 1:2203831/71‑1 (MQ=255) aaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGt > 1:1309809/1‑71 (MQ=255) aTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAAc > 1:2206485/1‑54 (MQ=255) gcgcAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGc > 1:1247391/1‑70 (MQ=255) gcAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGaa > 1:2681758/1‑71 (MQ=255) aGGATATCGTGTCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAAtt < 1:1397801/71‑1 (MQ=255) aGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAAtt < 1:2575712/71‑1 (MQ=255) ggATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTaa > 1:3481289/1‑50 (MQ=255) ggATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTa > 1:2754854/1‑71 (MQ=255) ggATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTa > 1:3924936/1‑71 (MQ=255) ggATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTa > 1:3247821/1‑71 (MQ=255) atatCGTGGCACGTACCATGCGCATCATCGATACCAATATCaa > 1:769975/1‑43 (MQ=255) tatCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTaa > 1:1138011/1‑47 (MQ=255) tCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGc < 1:2279779/71‑1 (MQ=255) gTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGAt > 1:2729775/1‑48 (MQ=255) gTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTAt > 1:1210975/1‑65 (MQ=255) gTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTaa > 1:2476994/1‑43 (MQ=255) tGGCACGTACCATGCGCATCATCGATACCAATATCAACGt < 1:3579647/40‑1 (MQ=255) gCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAgcg < 1:1052285/70‑1 (MQ=255) gCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAgcg < 1:1683525/70‑1 (MQ=255) gCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAgcg < 1:2784723/70‑1 (MQ=255) gCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAgcg < 1:2322379/70‑1 (MQ=255) cACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAgcg < 1:430505/69‑1 (MQ=255) aCGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAgcggc > 1:1650346/1‑70 (MQ=255) cGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGa < 1:3415986/71‑1 (MQ=255) cGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGa < 1:345944/71‑1 (MQ=255) ccATGCGCATCATCGATACCAATATCAACGTAATGGAt > 1:1526022/1‑38 (MQ=255) ccATGCGCATCATCGATACCAATAACAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt > 1:2303304/1‑71 (MQ=255) aTGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCg > 1:3155938/1‑55 (MQ=255) tGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAgcggcg < 1:2486683/63‑1 (MQ=255) gcgcATCATCGATACCAATATCAGCGTAATGGATGcca > 1:3493505/1‑37 (MQ=38) gcgcATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCa > 1:73963/1‑56 (MQ=255) cgcATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCg < 1:3892684/71‑1 (MQ=255) cgcATCATCGATACCAATATCAACGTTATGGATGCCCGTCGGCGAATTATCGGCAGCGGCGATCGTGAGCg < 1:2635551/71‑1 (MQ=255) cgcATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCa > 1:2549746/1‑55 (MQ=255) cgcATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCg > 1:1286090/1‑71 (MQ=255) cgcATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCg > 1:16913/1‑71 (MQ=255) cgcATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCg < 1:3721223/71‑1 (MQ=255) atcatcGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCTATCGTGAGCGTAt < 1:2030760/71‑1 (MQ=255) atcatcGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTAt < 1:2885557/71‑1 (MQ=255) atcatcGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTAt < 1:726024/71‑1 (MQ=255) tcatcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAAtt < 1:4064642/44‑1 (MQ=255) tcatcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAAtt < 1:2701521/44‑1 (MQ=255) tcatcGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAgcgg < 1:1490507/55‑1 (MQ=255) tcatcGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTg < 1:2236302/63‑1 (MQ=255) tcatcGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCg < 1:3405916/67‑1 (MQ=255) atcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTgg > 1:1622803/1‑71 (MQ=255) atcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTg < 1:726198/70‑1 (MQ=255) atcGATACCAATATCAACGTAATGGATGCCCGTGGGCGaa > 1:467900/1‑40 (MQ=255) atcGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTa > 1:434560/1‑67 (MQ=255) atcGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTg < 1:1874689/70‑1 (MQ=255) tcGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAgcg < 1:3218400/51‑1 (MQ=255) gATACCAATATCAACGTAATGGATGCCCGTGTGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGa < 1:817416/71‑1 (MQ=255) gATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg > 1:3985337/1‑70 (MQ=255) aTACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg < 1:2738449/69‑1 (MQ=255) aTACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGaa < 1:2873594/71‑1 (MQ=255) tACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGaa < 1:27798/70‑1 (MQ=255) tACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTa > 1:2257971/1‑62 (MQ=255) aCCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAAtt > 1:1903401/1‑71 (MQ=255) aCCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAAtt > 1:46290/1‑71 (MQ=255) ccAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAAtt < 1:2901978/70‑1 (MQ=255) ccAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTg < 1:3805255/71‑1 (MQ=255) ccAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTg < 1:1335111/71‑1 (MQ=255) aaTATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCa > 1:3711331/1‑71 (MQ=255) aaTATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCa > 1:2786819/1‑71 (MQ=255) atatCAGCGTAATGGATGCCCGTGGGCGAATTATc < 1:3819346/35‑1 (MQ=255) atatCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAgcg < 1:203743/42‑1 (MQ=255) tatCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAgc > 1:276787/1‑40 (MQ=255) tatCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTgg > 1:834217/1‑60 (MQ=255) tatCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg < 1:3341491/62‑1 (MQ=255) tatCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGa < 1:3463837/63‑1 (MQ=255) tCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTAtt > 1:1381079/1‑56 (MQ=255) tCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGt > 1:3014163/1‑53 (MQ=255) tCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACg > 1:1008719/1‑69 (MQ=255) tCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACGa < 1:3299361/70‑1 (MQ=255) tCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACGa < 1:1622707/70‑1 (MQ=255) cAACGTAATGGATGCCCGTGGGCGAATTATCTGCAGCGGCGATCGTGAg > 1:2314200/1‑49 (MQ=255) aaCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACGAAg < 1:3697499/70‑1 (MQ=255) | CATCTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACGAAG > minE/161300‑161435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |