| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,429,583 | C→T | 51.5% | intergenic (‑1/+11) | wzzE ← / ← rfe | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein/UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,429,583 | 0 | C | T | 51.5% | ‑6.4 / 101.7 | 68 | intergenic (‑1/+11) | wzzE/rfe | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein/UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase |
| Reads supporting (aligned to +/- strand): ref base C (22/11); new base T (18/17); total (40/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01 | |||||||||||
GAAACAACCCACGAATATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGTCTGCGTTT > minE/2429513‑2429644 | gAAACAACCCACGAATATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGtcatc < 1:1217625/71‑1 (MQ=255)gAAACAACCCACGAATATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGtcatc < 1:3357615/71‑1 (MQ=255) acaacCCACGAATATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATcaca < 1:2497980/71‑1 (MQ=255) acaacCCACGAATATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGGCATcaca < 1:3083184/71‑1 (MQ=255) ccACGAATATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCt > 1:2857595/1‑70 (MQ=255) aCGAATATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATc < 1:897479/66‑1 (MQ=255) cGAATATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCatt < 1:2605021/71‑1 (MQ=255) gAATATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCatt > 1:866975/1‑70 (MQ=255) gAATATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCattt < 1:1968126/71‑1 (MQ=255) aaTATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCattta > 1:2231221/1‑71 (MQ=255) cAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCatttattt > 1:1630472/1‑68 (MQ=255) gTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTa < 1:1879905/71‑1 (MQ=255) ttCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTaa < 1:3027059/71‑1 (MQ=255) tCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTaaa > 1:1016876/1‑71 (MQ=255) tCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTaaa > 1:2073768/1‑71 (MQ=255) cATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAAt > 1:1114122/1‑71 (MQ=255) cATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCat > 1:3343085/1‑57 (MQ=255) cATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAAt > 1:307901/1‑71 (MQ=255) aTTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAAtt < 1:2047133/71‑1 (MQ=255) aTTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAAtt < 1:1181469/71‑1 (MQ=255) ttttCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTg < 1:340166/71‑1 (MQ=255) ttCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTaaa > 1:2403389/1‑66 (MQ=255) ttCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTggg > 1:1985225/1‑71 (MQ=255) tCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCattta < 1:1283545/55‑1 (MQ=255) tCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCattta < 1:3475749/55‑1 (MQ=255) aGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTaaa > 1:1596698/1‑63 (MQ=255) aGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCt < 1:3612998/71‑1 (MQ=255) gCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTg > 1:2820533/1‑71 (MQ=255) ttCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAAtt > 1:3603648/1‑59 (MQ=255) ttCGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGccac > 1:1474593/1‑70 (MQ=255) ttCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGt > 1:1260525/1‑53 (MQ=255) ttCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGccac > 1:4076458/1‑70 (MQ=255) ttCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGccac > 1:965428/1‑70 (MQ=255) ttCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGccac > 1:180527/1‑70 (MQ=255) ttCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGcca > 1:2829185/1‑69 (MQ=255) cGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTaaa < 1:3532477/55‑1 (MQ=255) cGGCCGGTTTCCCAGGCATTGGTTGTGTCATTACATACTCATTTATTTGGTTAAATTGGGGCTGccac < 1:2088678/68‑1 (MQ=255) cGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCcaccac > 1:3668105/1‑71 (MQ=255) cGGCCGGTTTCCCACGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAAtt > 1:438139/1‑57 (MQ=255) ccGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGAt < 1:3921932/71‑1 (MQ=255) ccGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGAt < 1:559868/71‑1 (MQ=255) ccGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGAt < 1:3435702/71‑1 (MQ=255) ccGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGAt > 1:3051055/1‑71 (MQ=255) ccGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGAt > 1:357529/1‑71 (MQ=255) cGGTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGAtt > 1:209223/1‑71 (MQ=255) gTTTCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGc > 1:2000555/1‑59 (MQ=255) tttCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCt < 1:3339131/71‑1 (MQ=255) ttCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTa > 1:2831026/1‑71 (MQ=255) ttCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTa > 1:740132/1‑71 (MQ=255) ttCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTa > 1:3580721/1‑71 (MQ=255) tCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTAc > 1:165212/1‑71 (MQ=255) tCCCAGGCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTAc > 1:1611373/1‑71 (MQ=255) cAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAAt < 1:3702372/44‑1 (MQ=255) cAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTg > 1:1943288/1‑46 (MQ=255) cAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCa < 1:604324/71‑1 (MQ=255) aGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTa < 1:1767240/40‑1 (MQ=255) gCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGTc < 1:775066/71‑1 (MQ=255) gCATTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGTc > 1:830488/1‑71 (MQ=255) aTTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAAt > 1:625075/1‑39 (MQ=255) aTTGGTTGTGTCATTACATCCTCATTTATTTGGTTAAAt > 1:2390202/1‑39 (MQ=255) ttGGTTGTGTCATTACATCCTCATTTATTTGGTTaaa < 1:2980626/37‑1 (MQ=255) ttGGTTGTGTCATTACATCCTCATTTATTTGGTTaaa < 1:2798738/37‑1 (MQ=255) ttGGTTGTGTCATCACATCCTCATTTATTTGGTTaaa < 1:2456309/37‑1 (MQ=255) ttGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGTCTGc > 1:1163471/1‑71 (MQ=255) ggTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGTCTGCg < 1:2005736/70‑1 (MQ=255) ggTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGTCTGCGt > 1:2761087/1‑71 (MQ=255) ggTTGTGTCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGTCTGCGt > 1:2667749/1‑71 (MQ=255) gTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGTCTGCGt < 1:2946284/70‑1 (MQ=255) tgtgTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCcaccac > 1:1817994/1‑48 (MQ=255) gtgtCATCACATACTCATTTATTTGGTTAAATTGGGGCTGCCACAACGAt > 1:1216077/1‑50 (MQ=255) tgtCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGt < 1:3421657/60‑1 (MQ=255) tgtCATTACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGt < 1:2653066/60‑1 (MQ=255) tcatcaCATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGTCTGCGttt < 1:1911817/66‑1 (MQ=255) cacaTCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGTCTGCGtt > 1:2542540/1‑61 (MQ=255) | GAAACAACCCACGAATATCCAGTTCATTTTCAGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATTTATTTGGTTAAATTGGGGCTGCCACCACGATTTCTACGCAGTCTGCGTTT > minE/2429513‑2429644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |