| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,430,365 | T→C | 20.3% | I111M (ATA→ATG) | rfe ← | UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,430,365 | 0 | T | C | 20.3% | 121.6 / 28.2 | 59 | I111M (ATA→ATG) | rfe | UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase |
| Reads supporting (aligned to +/- strand): ref base T (22/25); new base C (5/7); total (27/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01 | |||||||||||
CGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAGC > minE/2430298‑2430434 | cGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACcatcat < 1:1972899/70‑1 (MQ=255) aGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATaa > 1:3638212/1‑70 (MQ=255) gCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACa < 1:535337/71‑1 (MQ=255) cACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACaa < 1:2709079/71‑1 (MQ=255) aTCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGcc < 1:3852260/71‑1 (MQ=255) aTCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGc > 1:2324286/1‑70 (MQ=255) aTCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGcc < 1:2193013/71‑1 (MQ=255) tctcCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGcc > 1:1684206/1‑70 (MQ=255) tcCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCaa > 1:801714/1‑70 (MQ=255) ccAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCa < 1:851718/67‑1 (MQ=255) ccAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCa < 1:640008/67‑1 (MQ=255) ccAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAg > 1:755134/1‑71 (MQ=255) gAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGc < 1:3335684/71‑1 (MQ=255) ccAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGcc < 1:2157545/59‑1 (MQ=255) cAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATtata < 1:4018712/50‑1 (MQ=255) cAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTg < 1:2435460/70‑1 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGc < 1:3667638/64‑1 (MQ=255) ataACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATtat > 1:2348637/1‑42 (MQ=255) ataACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCaa > 1:711358/1‑53 (MQ=255) ataACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCaa > 1:1452326/1‑53 (MQ=255) taACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGc < 1:1211150/71‑1 (MQ=255) aaCCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACaa > 1:3679981/1‑45 (MQ=255) aCCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCaa > 1:1664153/1‑50 (MQ=255) cccAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACg > 1:817342/1‑71 (MQ=255) aGGCTACTGAGATAAAGCTTGCCGAACACCATTATAAc < 1:3267636/38‑1 (MQ=255) tACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtttt > 1:1768805/1‑70 (MQ=255) cTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtttt < 1:2227868/68‑1 (MQ=255) cTGAGATAAAGCTTGCCGAACACCATCATAACAAt < 1:812836/35‑1 (MQ=255) gagaTAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTa > 1:1101803/1‑50 (MQ=255) gagaTAAAGCTTGCCGAACACCATCATAACAATgg > 1:3872973/1‑34 (MQ=39) gagaTAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtttt < 1:387455/66‑1 (MQ=255) agaTAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACg < 1:252153/69‑1 (MQ=255) agaTAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCt < 1:3053866/71‑1 (MQ=255) agaTAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCt > 1:312336/1‑71 (MQ=255) agaTAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCt > 1:3149675/1‑71 (MQ=255) agaTAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCt > 1:2446346/1‑71 (MQ=255) agaTAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCt < 1:2099386/71‑1 (MQ=255) aTAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTAt < 1:3105234/48‑1 (MQ=255) aTAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCt < 1:2857188/69‑1 (MQ=255) aaaGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGata > 1:1112984/1‑71 (MQ=255) aaGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGt < 1:1264065/43‑1 (MQ=255) aaGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGt < 1:231657/43‑1 (MQ=255) aaGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTg > 1:2964362/1‑67 (MQ=255) aGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTa > 1:266745/1‑43 (MQ=255) gCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTAt > 1:3970121/1‑43 (MQ=255) gCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTAt > 1:3974846/1‑43 (MQ=255) gCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACgg < 1:3915310/53‑1 (MQ=255) cTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtt < 1:453657/55‑1 (MQ=255) cTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtt < 1:4109656/55‑1 (MQ=255) cTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTa > 1:3129362/1‑59 (MQ=255) cTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCaa < 1:1441489/71‑1 (MQ=255) ttGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCt < 1:1273013/62‑1 (MQ=255) ccGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACg < 1:999760/71‑1 (MQ=255) ccGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACg > 1:139623/1‑71 (MQ=255) ccGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACg < 1:2310147/71‑1 (MQ=255) cGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACgg < 1:511115/71‑1 (MQ=255) cGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACgg < 1:3132776/71‑1 (MQ=255) aaCACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCa > 1:3342597/1‑42 (MQ=255) aaCACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACg > 1:3411496/1‑54 (MQ=255) aaCACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGatat < 1:2618568/61‑1 (MQ=255) cacCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTAAt > 1:2073666/1‑71 (MQ=255) ccATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATc > 1:3572934/1‑70 (MQ=255) ttATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGa > 1:2907773/1‑51 (MQ=255) ttATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAgc > 1:3904854/1‑71 (MQ=255) ttATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAgc > 1:3565195/1‑71 (MQ=255) | CGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAGC > minE/2430298‑2430434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |