| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,449,933 | A→G | 15.4% | S440G (AGT→GGT) | yifB → | predicted bifunctional protein, enzyme and transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,449,933 | 0 | A | G | 15.4% | 257.2 / 36.2 | 104 | S440G (AGT→GGT) | yifB | predicted bifunctional protein, enzyme and transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (48/40); new base G (10/6); total (58/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
AAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGATCCA > minE/2449864‑2450003 | aaaCAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAg > 1:1320283/1‑71 (MQ=255)aaaCAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAg > 1:156309/1‑71 (MQ=255) aacaacGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGt < 1:2049611/71‑1 (MQ=255) aacGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCg > 1:2868102/1‑71 (MQ=255) cgcgTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCgg > 1:2768149/1‑70 (MQ=255) cgcgTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCgg > 1:3299594/1‑70 (MQ=255) cgcgTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGa < 1:3639391/71‑1 (MQ=255) cgcgTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGa < 1:2499677/71‑1 (MQ=255) cgcgTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGa < 1:2243007/71‑1 (MQ=255) aTGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACg > 1:2536499/1‑71 (MQ=255) aTGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACg > 1:1605259/1‑71 (MQ=255) aTGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACg > 1:3176972/1‑71 (MQ=255) aTGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACg > 1:2844170/1‑71 (MQ=255) tGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAAt < 1:1114141/67‑1 (MQ=255) ggCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGc > 1:3129889/1‑70 (MQ=255) gccgccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGcc > 1:815965/1‑70 (MQ=255) gccgccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGACAGTCCGGAAATACGcc > 1:1886214/1‑70 (MQ=255) ccgccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCaa < 1:2825200/71‑1 (MQ=255) cgccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAAt < 1:1801135/71‑1 (MQ=255) gccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGa < 1:3336662/59‑1 (MQ=255) ccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGaa < 1:3884862/59‑1 (MQ=255) agagagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAAtt > 1:2839224/1‑68 (MQ=255) agagagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAAtt > 1:3937567/1‑68 (MQ=255) agagagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTg < 1:896620/71‑1 (MQ=255) gagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTg > 1:844821/1‑68 (MQ=255) agCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCaaa > 1:1370017/1‑71 (MQ=255) agCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCaaa > 1:1266917/1‑71 (MQ=255) cgcCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACt > 1:2814344/1‑71 (MQ=255) gcCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACtt < 1:2773648/71‑1 (MQ=255) ccAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGaaa < 1:1648160/50‑1 (MQ=255) ccAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTg < 1:1018421/71‑1 (MQ=255) cAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACtt < 1:1745012/69‑1 (MQ=255) cAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACtt > 1:3793019/1‑69 (MQ=255) cAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACtt > 1:3788143/1‑69 (MQ=255) aTTTAAGCGGCAGAATAAACTGAA‑‑‑‑TGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg > 1:1323374/1‑70 (MQ=255) tttAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGaaa < 1:2847142/46‑1 (MQ=255) tttAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGagag > 1:3373582/1‑71 (MQ=255) tAAGCGGCAGAATAAACTGAATGCCTGGCTGGATa > 1:2835218/1‑35 (MQ=255) tAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:2507420/1‑66 (MQ=255) aGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGaa < 1:3793194/71‑1 (MQ=255) gCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg > 1:35075/1‑68 (MQ=255) gCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAg < 1:678320/71‑1 (MQ=255) cGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGa < 1:3301652/71‑1 (MQ=255) ggCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGAt < 1:2094757/71‑1 (MQ=255) ggCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGAt < 1:535652/71‑1 (MQ=255) ggCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGAt < 1:56450/71‑1 (MQ=255) ggCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGAt < 1:1240123/71‑1 (MQ=255) gCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACtt < 1:3742470/58‑1 (MQ=255) gCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGAt < 1:1337716/70‑1 (MQ=255) cAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGc < 1:2633790/71‑1 (MQ=255) cAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGc < 1:1711824/71‑1 (MQ=255) gAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGa > 1:2187535/1‑71 (MQ=255) aaTAAACTGAATGCCTGGCTGGATAGTCCGGAAAt < 1:543577/35‑1 (MQ=255) aTAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAtg > 1:1868069/1‑71 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:2245683/1‑54 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:3038312/1‑54 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAtgt > 1:3608977/1‑71 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAtgt > 1:395288/1‑71 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAtgt > 1:463356/1‑71 (MQ=255) aaaCTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAtgtg > 1:1302766/1‑71 (MQ=255) aaCTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACtt < 1:1344115/50‑1 (MQ=255) aaCTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg < 1:2929964/57‑1 (MQ=255) aaCTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGAt < 1:682569/62‑1 (MQ=255) aaCTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAt > 1:3643311/1‑67 (MQ=255) aaCTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTgg > 1:1147316/1‑71 (MQ=255) aCTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:4062354/1‑51 (MQ=255) cTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGtt > 1:136739/1‑71 (MQ=255) tGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGaa < 1:1286044/56‑1 (MQ=255) tGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGaa < 1:1241316/56‑1 (MQ=255) gAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTg > 1:939182/1‑70 (MQ=255) gAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTg > 1:347627/1‑70 (MQ=255) gAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTg > 1:1230715/1‑70 (MQ=255) gAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTg > 1:2743023/1‑70 (MQ=255) gAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTg > 1:432959/1‑70 (MQ=255) aaTGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTgg < 1:632241/70‑1 (MQ=255) aaTGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTgg > 1:3330705/1‑70 (MQ=255) aaTGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTg < 1:3678144/69‑1 (MQ=255) tGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaag > 1:3088143/1‑71 (MQ=255) tGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaag > 1:543631/1‑71 (MQ=255) tGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTgg < 1:650273/68‑1 (MQ=255) ccTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAAc > 1:1175857/1‑39 (MQ=255) ccTGGCTGGATAGTCCGGTAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaagg < 1:3594535/70‑1 (MQ=255) ccTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaagg > 1:137892/1‑70 (MQ=255) ccTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaagg > 1:272299/1‑70 (MQ=255) ccTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaagg > 1:1185618/1‑70 (MQ=255) ccTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaagg > 1:3396066/1‑70 (MQ=255) ctggctggATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaaggaa < 1:1758075/71‑1 (MQ=255) gctggATAGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:1350333/1‑39 (MQ=255) gctggATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGa > 1:3444286/1‑48 (MQ=255) gctggATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAaca > 1:2430758/1‑70 (MQ=255) ggATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg < 1:532320/41‑1 (MQ=255) ggATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg < 1:835333/41‑1 (MQ=255) gATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGa > 1:1007322/1‑44 (MQ=255) gATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTg < 1:424856/69‑1 (MQ=255) gATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTg < 1:1411487/69‑1 (MQ=255) aTAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGAt < 1:422952/44‑1 (MQ=255) aTAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAt > 1:3692367/1‑49 (MQ=255) aTAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGATc > 1:2358853/1‑71 (MQ=255) aGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGaa > 1:1551162/1‑39 (MQ=255) aGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGATCCa > 1:3456261/1‑71 (MQ=255) | AAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGATCCA > minE/2449864‑2450003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |