| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,493,676 | T→C | 5.6% | intergenic (+58/‑317) | gyrB → / → ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,493,676 | 0 | T | C | 5.6% | 205.7 / 3.5 | 71 | intergenic (+58/‑317) | gyrB/ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Reads supporting (aligned to +/- strand): ref base T (27/40); new base C (2/2); total (29/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
GATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGC > minE/2493610‑2493744 | gATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGc < 1:541102/70‑1 (MQ=255) tatTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCt > 1:824598/1‑65 (MQ=255) atTTAATGGCGCTAACCATGCGAGCATGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATg > 1:2650945/1‑71 (MQ=255) tttAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTg < 1:1124758/65‑1 (MQ=255) tttAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTg < 1:2866108/65‑1 (MQ=255) tttAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATg < 1:2886743/70‑1 (MQ=255) tAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTa < 1:708616/70‑1 (MQ=255) tAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTAt > 1:3550996/1‑71 (MQ=255) aTGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCt < 1:2867088/59‑1 (MQ=255) cTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAtttt < 1:1960570/71‑1 (MQ=255) aCCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTc < 1:1710278/69‑1 (MQ=255) cATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAAt < 1:1362020/54‑1 (MQ=255) aTGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAt < 1:988532/71‑1 (MQ=255) tGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTg < 1:3020651/58‑1 (MQ=255) gAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:1095152/71‑1 (MQ=255) gAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:3783501/71‑1 (MQ=255) gCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:72727/69‑1 (MQ=255) aatGCCTGATGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAg > 1:3604071/3‑71 (MQ=14) gCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGcc > 1:487445/1‑71 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCg < 1:254587/71‑1 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGa > 1:2416568/1‑71 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCaa < 1:3937020/38‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt < 1:2665850/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt < 1:2749763/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt < 1:727083/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATa < 1:880146/71‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGCAGGCCGGAt < 1:3768838/70‑1 (MQ=255) tGCGCTACGCTTATCAGGCCTACGAAAATCTTGCaa > 1:2286396/1‑36 (MQ=255) tGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt > 1:3493317/1‑69 (MQ=255) gcgcTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGAtt > 1:2329515/1‑55 (MQ=14) cgcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAg < 1:3378400/70‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAg < 1:398353/70‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAg < 1:2201592/70‑1 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGa < 1:1335706/50‑1 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg > 1:3079603/1‑55 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg > 1:3047426/1‑55 (MQ=255) aCGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAttt > 1:1628117/1‑51 (MQ=255) aCGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAt < 1:3073025/49‑1 (MQ=255) cGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGtt < 1:667801/70‑1 (MQ=14) ttATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGc < 1:3003743/54‑1 (MQ=255) aTCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTc < 1:400982/38‑1 (MQ=255) tCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCa > 1:3205847/1‑66 (MQ=255) tCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCa > 1:1305930/1‑66 (MQ=255) cAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGtt < 1:1900159/63‑1 (MQ=14) aGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAtt > 1:1809563/1‑41 (MQ=255) aGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:4085840/44‑1 (MQ=255) gCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTa > 1:3320923/1‑44 (MQ=255) cTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:3170884/1‑71 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:2933856/39‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCg < 1:64223/46‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt < 1:1630983/49‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATaa < 1:927463/51‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGt < 1:2464765/56‑1 (MQ=255) aCGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCg < 1:2654890/45‑1 (MQ=255) aCGAAAATCTTGAAATGTATTGAATTTTCATGAttt > 1:3405042/1‑36 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCg < 1:1911285/44‑1 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:2445799/1‑70 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:2406494/1‑70 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:3665950/1‑70 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc < 1:1365970/70‑1 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc < 1:3234391/70‑1 (MQ=255) gAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:2276484/1‑69 (MQ=255) gAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:2897441/1‑69 (MQ=255) gAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:3433086/1‑69 (MQ=255) aaaaTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATaa > 1:4049445/1‑47 (MQ=255) aaaaTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:1692379/1‑68 (MQ=255) aaaTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaaga < 1:965684/71‑1 (MQ=255) aaaTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaaga < 1:3028915/71‑1 (MQ=255) aaaTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaaga < 1:3023940/71‑1 (MQ=255) aaaTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaag > 1:1688900/1‑70 (MQ=255) aaaTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaag > 1:3892193/1‑70 (MQ=255) tCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGc > 1:1268453/1‑71 (MQ=255) tCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGc > 1:2905031/1‑71 (MQ=255) ttGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGa > 1:2690652/1‑38 (MQ=255) ttGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAcgcc > 1:3996887/1‑53 (MQ=255) ttGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1854759/1‑58 (MQ=255) | GATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGC > minE/2493610‑2493744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |