| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,766 | C→T | 5.2% | I12I (ATC→ATT) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,766 | 0 | C | T | 5.2% | 386.1 / 6.9 | 115 | I12I (ATC→ATT) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (45/64); new base T (3/3); total (48/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
AAAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGC > minE/2569696‑2569836 | aaaCAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt < 1:2409148/71‑1 (MQ=255) cacTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCt > 1:2133539/1‑71 (MQ=255) cacTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCt > 1:2572042/1‑71 (MQ=255) aTAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc < 1:2964011/69‑1 (MQ=255) aTAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg > 1:2931047/1‑71 (MQ=255) tAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt > 1:577363/1‑60 (MQ=255) tAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTgg > 1:3416980/1‑71 (MQ=255) gTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt < 1:754041/67‑1 (MQ=255) tGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtt > 1:2581225/1‑70 (MQ=255) gAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttt > 1:1702438/1‑71 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:1372364/71‑1 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt > 1:2349709/1‑71 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:2087667/71‑1 (MQ=255) aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:800246/70‑1 (MQ=255) tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttttt < 1:44690/71‑2 (MQ=255) cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt < 1:1467747/70‑1 (MQ=255) gAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCt < 1:1664792/52‑1 (MQ=255) gAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg > 1:1072949/1‑70 (MQ=255) aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa < 1:2242740/71‑1 (MQ=255) ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa > 1:441251/1‑71 (MQ=255) gCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAat > 1:1421227/1‑71 (MQ=255) cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc < 1:1249965/71‑1 (MQ=255) aGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca < 1:3138156/71‑1 (MQ=255) atatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCtt < 1:1347113/46‑1 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCt > 1:1232930/1‑43 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc < 1:2240453/68‑1 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:3521780/70‑1 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:3017378/70‑1 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:3233839/70‑1 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:4075895/70‑1 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:3179990/70‑1 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTCTATGGAATCATcgc < 1:2246018/70‑1 (MQ=255) gTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa < 1:1200291/57‑1 (MQ=255) tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCt > 1:3951143/1‑40 (MQ=255) tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcat > 1:3175647/1‑65 (MQ=255) tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:434875/1‑70 (MQ=255) tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:2882868/71‑1 (MQ=255) tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:2833087/71‑1 (MQ=255) tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTc > 1:1026480/1‑37 (MQ=255) tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa > 1:3845027/1‑70 (MQ=255) tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa > 1:828499/1‑70 (MQ=255) cTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc < 1:2036234/70‑1 (MQ=255) tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:3398568/50‑1 (MQ=255) tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa < 1:571478/68‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg > 1:599103/1‑53 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt < 1:886056/69‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATg < 1:3787266/70‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa > 1:1823857/1‑71 (MQ=255) aCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa > 1:2775/1‑70 (MQ=255) cTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAat > 1:3611882/1‑54 (MQ=255) aTTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc > 1:1979739/1‑56 (MQ=255) tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:3980854/43‑1 (MQ=255) tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg < 1:1750753/47‑1 (MQ=255) tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa > 1:2435532/1‑63 (MQ=255) ttCGCGTTGGGAGTGCTGATCTTCATCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:1363441/1‑58 (MQ=255) tCGCGTTGGGAGTGCTGATCTTCGTGTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATccc < 1:3340411/70‑1 (MQ=255) tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt > 1:3643282/1‑71 (MQ=255) tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt > 1:3208841/1‑71 (MQ=255) gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:2896994/70‑1 (MQ=255) gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTa < 1:55507/71‑1 (MQ=255) cgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt < 1:3671463/59‑1 (MQ=255) gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:3763719/68‑1 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2183125/71‑1 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:1768938/71‑1 (MQ=255) tGGGAGTGCTTATTTTTGTATTTCTGGTGAtcttc < 1:1907118/35‑2 (MQ=12) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa > 1:2425972/1‑58 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:3208538/1‑50 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTAt > 1:2254890/1‑67 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg < 1:422988/70‑1 (MQ=255) ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcatta > 1:2932823/1‑44 (MQ=255) ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcatta > 1:2780488/1‑44 (MQ=255) ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt > 1:3566006/1‑71 (MQ=255) ggAGTGCTGAGCTTCGTCTTTCTGGTGATTTTTTATGGa < 1:4128823/39‑1 (MQ=255) gAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAtt < 1:1484990/71‑1 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2914095/65‑1 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:4062136/65‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:3062570/43‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:1358500/43‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:1308024/45‑1 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:3804067/1‑43 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc > 1:3109888/1‑46 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGcc > 1:1109102/1‑69 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa > 1:1175868/1‑70 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa > 1:459046/1‑70 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:3982411/40‑1 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:2488033/1‑42 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa < 1:1380459/44‑1 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa < 1:1804673/49‑1 (MQ=255) gATCTTCGTCTTTCTTGTGATTTTTTATGGAatcatc < 1:2297045/37‑1 (MQ=255) gATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa > 1:2854270/1‑70 (MQ=255) gATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa > 1:2101051/1‑70 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:373378/38‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:3968009/38‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa < 1:1590406/70‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa < 1:3107318/69‑1 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt > 1:2701841/1‑43 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa > 1:2901621/1‑47 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgc > 1:155227/1‑71 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgc > 1:2523379/1‑71 (MQ=255) cTTAGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt > 1:4114129/1‑43 (MQ=255) | AAAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGC > minE/2569696‑2569836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |