| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,841 | C→T | 5.4% | N37N (AAC→AAT) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,841 | 0 | C | T | 5.4% | 309.3 / 4.9 | 92 | N37N (AAC→AAT) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (46/41); new base T (3/2); total (49/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
TCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATC > minE/2569771‑2569904 | tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc < 1:3143304/71‑1 (MQ=255) ttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCAt > 1:607700/1‑71 (MQ=255) tCTGGTGATTTTTTATGTAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc < 1:556096/68‑1 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:139874/1‑67 (MQ=255) cTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:1418892/1‑71 (MQ=255) cTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc < 1:3615630/70‑1 (MQ=255) cTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc < 1:3232209/70‑1 (MQ=255) cTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc < 1:77264/70‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc < 1:37745/70‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:4026137/69‑1 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:3290944/1‑71 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt < 1:621373/71‑1 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt < 1:1401956/71‑1 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:2614097/1‑71 (MQ=255) gATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAATCATcccc > 1:1274487/1‑68 (MQ=255) gATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc > 1:2158427/1‑68 (MQ=255) ttttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:1132263/71‑1 (MQ=255) tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:452812/1‑58 (MQ=255) tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc > 1:3632706/1‑70 (MQ=255) aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc > 1:3986902/1‑70 (MQ=255) aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc > 1:462296/1‑70 (MQ=255) aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc < 1:1134066/70‑1 (MQ=255) tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc < 1:1363809/69‑1 (MQ=255) tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCt < 1:2703948/70‑1 (MQ=255) tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa < 1:3212536/71‑1 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:262934/70‑1 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:4057028/70‑1 (MQ=255) atcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCa > 1:2761807/1‑56 (MQ=255) atcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCa > 1:1354956/1‑56 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCTCAACCATCCCCATGCCGACGCTAtt < 1:1205087/68‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc < 1:848391/48‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:3473913/1‑70 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt < 1:3369987/71‑1 (MQ=255) catcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc > 1:1370937/1‑52 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc < 1:1519897/45‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:33530/1‑64 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg < 1:3889651/71‑1 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcgg > 1:3130024/1‑71 (MQ=255) gcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc > 1:2063193/1‑71 (MQ=255) gcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc > 1:2343356/1‑71 (MQ=255) cgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg < 1:1529839/71‑1 (MQ=255) gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt < 1:2515772/61‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:1447604/50‑1 (MQ=255) cATGAGATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:2952413/1‑71 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt < 1:3340647/70‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:3311068/1‑71 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:1367069/1‑71 (MQ=255) tGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc < 1:2246928/35‑1 (MQ=255) tGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc < 1:3143852/35‑1 (MQ=255) tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg > 1:356224/1‑71 (MQ=255) tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg < 1:2255860/71‑1 (MQ=255) gACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc > 1:2638771/1‑37 (MQ=255) gACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:2772236/1‑71 (MQ=255) aCATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:4112618/1‑70 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa < 1:3492799/52‑1 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt < 1:193167/67‑1 (MQ=255) cccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:1407391/1‑51 (MQ=255) cccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:4085203/1‑51 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:2167772/1‑46 (MQ=255) cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg < 1:2881446/71‑1 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:2598525/37‑1 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa < 1:3689817/39‑1 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt < 1:1027894/46‑1 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGt > 1:197004/1‑71 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2969052/1‑37 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2277523/1‑37 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:930878/1‑37 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCt > 1:3012275/1‑41 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc > 1:1891499/1‑45 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:2480107/1‑48 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt > 1:990417/1‑70 (MQ=255) tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:3233766/1‑47 (MQ=255) tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGttt < 1:1208724/70‑1 (MQ=255) cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:1038683/1‑46 (MQ=255) cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:1762898/1‑46 (MQ=255) cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:2361629/1‑46 (MQ=255) cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCAtgt < 1:725454/47‑3 (MQ=255) cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc < 1:1610422/71‑1 (MQ=255) tGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc > 1:1228502/1‑70 (MQ=255) tGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc > 1:1917940/1‑70 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGc > 1:455242/1‑35 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt < 1:2231458/39‑1 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa < 1:2023526/41‑1 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTa < 1:3277672/67‑1 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa < 1:2592923/40‑1 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:1277890/1‑40 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:1638252/1‑71 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg < 1:514100/71‑1 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg < 1:1446602/71‑1 (MQ=255) tGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAc > 1:2306928/1‑42 (MQ=255) gCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:201459/1‑40 (MQ=255) gCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg > 1:2166476/1‑45 (MQ=255) gCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCa > 1:2509197/1‑71 (MQ=255) ccAAAAAGCGCAACCATCCCCATGCCGACTCTATTCa < 1:1533640/37‑1 (MQ=255) cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCAt < 1:2097289/70‑1 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg < 1:2705871/69‑1 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt < 1:1744519/71‑1 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTa > 1:2790158/1‑70 (MQ=255) cgcgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:2449483/70‑1 (MQ=255) gcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt < 1:521538/52‑1 (MQ=255) | TCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATC > minE/2569771‑2569904 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |