| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,859 | T→C | 8.6% | A43A (GCT→GCC) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,859 | 0 | T | C | 8.6% | 262.5 / 13.8 | 93 | A43A (GCT→GCC) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (46/39); new base C (3/5); total (49/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
TGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGC > minE/2569790‑2569927 | tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCt < 1:2703948/70‑1 (MQ=255)tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa < 1:3212536/71‑1 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:262934/70‑1 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:4057028/70‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCTCAACCATCCCCATGCCGACGCTAtt < 1:1205087/68‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:3473913/1‑70 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt < 1:3369987/71‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:33530/1‑64 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg < 1:3889651/71‑1 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcgg > 1:3130024/1‑71 (MQ=255) gcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc > 1:2343356/1‑71 (MQ=255) gcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc > 1:2063193/1‑71 (MQ=255) cgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg < 1:1529839/71‑1 (MQ=255) gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt < 1:2515772/61‑1 (MQ=255) cATGAGATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:2952413/1‑71 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt < 1:3340647/70‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:3311068/1‑71 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:1367069/1‑71 (MQ=255) tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg > 1:356224/1‑71 (MQ=255) tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg < 1:2255860/71‑1 (MQ=255) gACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:2772236/1‑71 (MQ=255) aCATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:4112618/1‑70 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa < 1:3492799/52‑1 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt < 1:193167/67‑1 (MQ=255) cccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:1407391/1‑51 (MQ=255) cccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:4085203/1‑51 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:2167772/1‑46 (MQ=255) cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg < 1:2881446/71‑1 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt < 1:1027894/46‑1 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGt > 1:197004/1‑71 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCt > 1:3012275/1‑41 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc > 1:1891499/1‑45 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:2480107/1‑48 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt > 1:990417/1‑70 (MQ=255) tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:3233766/1‑47 (MQ=255) tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGttt < 1:1208724/70‑1 (MQ=255) cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:1038683/1‑46 (MQ=255) cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:1762898/1‑46 (MQ=255) cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:2361629/1‑46 (MQ=255) cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCAtgt < 1:725454/47‑3 (MQ=255) cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc < 1:1610422/71‑1 (MQ=255) tGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc > 1:1228502/1‑70 (MQ=255) tGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc > 1:1917940/1‑70 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt < 1:2231458/39‑1 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa < 1:2023526/41‑1 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTa < 1:3277672/67‑1 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:1277890/1‑40 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa < 1:2592923/40‑1 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg < 1:1446602/71‑1 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:1638252/1‑71 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg < 1:514100/71‑1 (MQ=255) tGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAc > 1:2306928/1‑42 (MQ=255) gCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:201459/1‑40 (MQ=255) gCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg > 1:2166476/1‑45 (MQ=255) gCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCa > 1:2509197/1‑71 (MQ=255) ccAAAAAGCGCAACCATCCCCATGCCGACTCTATTCa < 1:1533640/37‑1 (MQ=255) cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCAt < 1:2097289/70‑1 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg < 1:2705871/69‑1 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt < 1:1744519/71‑1 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTa > 1:2790158/1‑70 (MQ=255) cgcgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:2449483/70‑1 (MQ=255) gcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt < 1:521538/52‑1 (MQ=255) atcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCg > 1:2212166/3‑71 (MQ=255) cATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt < 1:3314130/70‑1 (MQ=255) tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc < 1:1419141/71‑1 (MQ=255) cccATGCCGACGCTATTCATACGGCGGTCTGGGTGAGCCTGTTTACTCTGCATGtt > 1:3788282/1‑56 (MQ=255) cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt < 1:708545/70‑1 (MQ=255) tcATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt < 1:3151205/53‑1 (MQ=255) ccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc < 1:939965/68‑1 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGttt > 1:4115960/1‑66 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:3910992/1‑71 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:1575045/1‑71 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:1727044/1‑71 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgt > 1:2191511/1‑70 (MQ=255) aaTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:502231/2‑71 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTTAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:194766/1‑70 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt < 1:1738963/52‑1 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg < 1:633390/71‑1 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg < 1:427964/71‑1 (MQ=255) tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:1332663/1‑69 (MQ=255) ccGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGttt > 1:1139567/1‑62 (MQ=255) ccGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:210277/1‑71 (MQ=255) ccGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:2339690/1‑71 (MQ=255) cGACTCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCt < 1:2711162/71‑1 (MQ=255) gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCa > 1:2551776/1‑44 (MQ=255) gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg < 1:2407866/71‑1 (MQ=255) gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg < 1:225772/71‑1 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:2201061/1‑68 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:1413131/1‑68 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:3326168/1‑68 (MQ=255) aCGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTg < 1:664420/70‑1 (MQ=255) aCGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTg < 1:2047322/70‑1 (MQ=255) gCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtgag < 1:2844112/64‑3 (MQ=255) gCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGc > 1:1555806/1‑71 (MQ=255) gCTATTCATACGGCGGGCTGGCGGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtgag < 1:811604/64‑3 (MQ=255) tATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt < 1:599802/42‑1 (MQ=255) | TGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGC > minE/2569790‑2569927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |