| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,871 | G→T | 9.5% | A47A (GCG→GCT) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,871 | 0 | G | T | 9.5% | 231.1 / 5.8 | 72 | A47A (GCG→GCT) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (35/30); new base T (3/4); total (38/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAC > minE/2569801‑2569941 | cgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg < 1:1529839/71‑1 (MQ=255) cATGAGATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:2952413/1‑71 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt < 1:3340647/70‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:3311068/1‑71 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:1367069/1‑71 (MQ=255) tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg > 1:356224/1‑71 (MQ=255) tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg < 1:2255860/71‑1 (MQ=255) gACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:2772236/1‑71 (MQ=255) aCATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:4112618/1‑70 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt < 1:193167/67‑1 (MQ=255) cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg < 1:2881446/71‑1 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGt > 1:197004/1‑71 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt > 1:990417/1‑70 (MQ=255) tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGttt < 1:1208724/70‑1 (MQ=255) cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc < 1:1610422/71‑1 (MQ=255) tGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc > 1:1917940/1‑70 (MQ=255) tGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc > 1:1228502/1‑70 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTa < 1:3277672/67‑1 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:1638252/1‑71 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg < 1:1446602/71‑1 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg < 1:514100/71‑1 (MQ=255) gCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg > 1:2166476/1‑45 (MQ=255) gCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCa > 1:2509197/1‑71 (MQ=255) cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCAt < 1:2097289/70‑1 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg < 1:2705871/69‑1 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt < 1:1744519/71‑1 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTa > 1:2790158/1‑70 (MQ=255) cgcgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:2449483/70‑1 (MQ=255) gcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt < 1:521538/52‑1 (MQ=255) atcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCg > 1:2212166/3‑71 (MQ=255) cATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt < 1:3314130/70‑1 (MQ=255) tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc < 1:1419141/71‑1 (MQ=255) cccATGCCGACGCTATTCATACGGCGGTCTGGGTGAGCCTGTTTACTCTGCATGtt > 1:3788282/1‑56 (MQ=255) cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt < 1:708545/70‑1 (MQ=255) tcATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt < 1:3151205/53‑1 (MQ=255) ccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc < 1:939965/68‑1 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGttt > 1:4115960/1‑66 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:1575045/1‑71 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:1727044/1‑71 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:3910992/1‑71 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgt > 1:2191511/1‑70 (MQ=255) aaTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:502231/2‑71 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTTAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:194766/1‑70 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt < 1:1738963/52‑1 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg < 1:427964/71‑1 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg < 1:633390/71‑1 (MQ=255) tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:1332663/1‑69 (MQ=255) ccGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGttt > 1:1139567/1‑62 (MQ=255) ccGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:210277/1‑71 (MQ=255) ccGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:2339690/1‑71 (MQ=255) cGACTCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCt < 1:2711162/71‑1 (MQ=255) gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCa > 1:2551776/1‑44 (MQ=255) gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg < 1:225772/71‑1 (MQ=255) gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg < 1:2407866/71‑1 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:1413131/1‑68 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:2201061/1‑68 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:3326168/1‑68 (MQ=255) aCGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTg < 1:664420/70‑1 (MQ=255) aCGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTg < 1:2047322/70‑1 (MQ=255) gCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtgag < 1:2844112/64‑3 (MQ=255) gCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGc > 1:1555806/1‑71 (MQ=255) gCTATTCATACGGCGGGCTGGCGGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtgag < 1:811604/64‑3 (MQ=255) tATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt < 1:599802/42‑1 (MQ=255) aTTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGa < 1:4035978/61‑1 (MQ=255) aTTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGAc > 1:4000188/1‑71 (MQ=255) aTTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGAc > 1:3525466/1‑71 (MQ=255) ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg < 1:2566614/64‑1 (MQ=255) cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCa > 1:307500/1‑35 (MQ=255) cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCa > 1:1275227/1‑35 (MQ=255) cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGct > 1:2754663/1‑71 (MQ=255) cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGc > 1:670692/1‑70 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc < 1:1827599/71‑1 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:650214/1‑71 (MQ=255) tACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg < 1:1251511/55‑1 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgt > 1:2853821/1‑51 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgt > 1:3424403/1‑51 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgt > 1:3263133/1‑51 (MQ=255) cggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGct < 1:1857656/64‑1 (MQ=255) cggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGct < 1:1708824/64‑1 (MQ=255) gggCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAc < 1:222475/71‑1 (MQ=255) | CGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAC > minE/2569801‑2569941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |