| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,427 | T→A | 5.1% | V482V (GTA→GTT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,427 | 0 | T | A | 5.1% | 231.7 / 3.5 | 79 | V482V (GTA→GTT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (40/35); new base A (2/2); total (42/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCA > minE/311359‑311496 | tGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTAc > 1:2016078/1‑71 (MQ=255)tGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTAc > 1:442773/1‑71 (MQ=255) aTCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTAcc > 1:3283865/1‑70 (MQ=255) aTCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTAcc > 1:1667070/1‑70 (MQ=255) aTCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTAcc < 1:2097881/70‑1 (MQ=255) aTCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTAcc > 1:1611210/1‑70 (MQ=255) aTCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTAcc > 1:2741941/1‑70 (MQ=255) aTCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTAcc > 1:3489411/1‑70 (MQ=255) aTCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTAcc > 1:382858/1‑70 (MQ=255) aTCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTAcc > 1:441084/1‑70 (MQ=255) cGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGa > 1:2675018/1‑70 (MQ=255) gCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTTGAGTCAGGATCAACGCCACCAGTACCGACa < 1:837090/71‑1 (MQ=255) gCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTa < 1:2726375/65‑1 (MQ=255) gCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGa > 1:359258/1‑69 (MQ=255) tttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAgcg > 1:1413060/1‑71 (MQ=255) ggCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGcc < 1:3797385/70‑1 (MQ=255) tCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCATGATCAACGCCACCAGTACCGACAGCGCCa > 1:2586122/1‑66 (MQ=255) tCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCCCCAGTAc > 1:4108672/1‑55 (MQ=255) tCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTAcc < 1:3076650/56‑1 (MQ=255) tCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACATCGcc > 1:1030651/1‑65 (MQ=255) cGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGa > 1:100972/1‑57 (MQ=255) ggTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTg > 1:876342/1‑70 (MQ=255) ggTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTg > 1:1291885/1‑70 (MQ=255) tCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAAc < 1:1169174/70‑1 (MQ=255) tCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAAc < 1:1858629/70‑1 (MQ=255) tCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAAc > 1:2111685/1‑70 (MQ=255) tCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAAc > 1:3133498/1‑70 (MQ=255) tCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAAc < 1:4109940/70‑1 (MQ=255) aGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAAt < 1:1121023/71‑1 (MQ=255) aGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAAt > 1:1570549/1‑71 (MQ=255) aGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAAt > 1:268024/1‑71 (MQ=255) aTGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATgg > 1:792499/1‑70 (MQ=255) aTGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGt < 1:3270665/71‑1 (MQ=255) gtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTaa < 1:3944650/70‑1 (MQ=255) gCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAAt > 1:1696424/1‑62 (MQ=255) gCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATa > 1:2363516/1‑69 (MQ=255) gCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATa > 1:1001928/1‑69 (MQ=255) cacaAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTaa < 1:2640482/66‑1 (MQ=255) acaAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAgcgc < 1:2569232/45‑1 (MQ=255) acaAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGa < 1:1627369/54‑1 (MQ=255) acaAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGa < 1:1246194/54‑1 (MQ=255) caAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTg < 1:1773358/49‑1 (MQ=255) aaaGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGa < 1:3397926/52‑1 (MQ=255) aaaGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGa < 1:3044367/52‑1 (MQ=255) aaaGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAAc < 1:958860/55‑1 (MQ=255) gcgCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGa > 1:181615/3‑71 (MQ=255) agCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc < 1:1056014/71‑1 (MQ=255) gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCa > 1:1183668/1‑36 (MQ=255) gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGa > 1:342043/1‑65 (MQ=255) gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgatag < 1:115343/71‑1 (MQ=255) gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgat < 1:3740230/69‑1 (MQ=255) aGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAAt < 1:10949/48‑1 (MQ=255) aGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGatagatag > 1:3684563/1‑70 (MQ=255) aGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGatagatag > 1:362022/1‑70 (MQ=255) gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATa < 1:2757028/49‑1 (MQ=255) tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGt < 1:543723/43‑1 (MQ=255) tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATaga > 1:425825/1‑49 (MQ=255) tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:1124834/1‑69 (MQ=255) tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:3731864/1‑69 (MQ=255) tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCa < 1:3324/71‑1 (MQ=255) cAATGCCACCAGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAgaga < 1:324753/50‑1 (MQ=255) cAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACga > 1:941713/1‑58 (MQ=255) aatgCCACCAGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAg > 1:1952394/4‑70 (MQ=9) aatgCCACCAGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGAGATAAATTGCCCCAg > 1:239808/4‑70 (MQ=9) aaCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATagag < 1:1619759/48‑1 (MQ=255) aaCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg < 1:3192083/70‑1 (MQ=255) aCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACg < 1:2226893/55‑1 (MQ=255) tgCCACCAGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAgaga < 1:3478613/46‑1 (MQ=19) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:2575960/71‑1 (MQ=255) gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCa > 1:398186/1‑66 (MQ=255) gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTa > 1:3591795/1‑69 (MQ=255) ccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATagag < 1:1045963/41‑1 (MQ=255) cagaacagaaAGCGCCATTGCCGAAACGATGGTGATAGAGAAc > 1:76246/1‑43 (MQ=16) caGTACCGACAGCGCCATTGCTGAAACAATGGTAATATAGAACTGACgat < 1:2672565/50‑1 (MQ=255) caGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgat < 1:2252326/50‑1 (MQ=255) caGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACga > 1:976084/1‑49 (MQ=255) aGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc > 1:3109767/1‑46 (MQ=255) aGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc > 1:1847989/1‑46 (MQ=255) gTACCGACAGCGCCATTGCTGAAACAATGGTAATag < 1:3117052/36‑1 (MQ=255) gTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa < 1:798959/71‑1 (MQ=255) tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc < 1:742265/44‑1 (MQ=255) tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:1599318/62‑1 (MQ=255) tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:3384108/62‑1 (MQ=255) | TGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCA > minE/311359‑311496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |