Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,570,774	A→G	6.5%	N227D (AAT→GAT)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,570,774	0	A	G	6.5%	221.7 / 6.1	77	N227D (AAT→GAT)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/39);  new base G (2/3);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

TGAGCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGT  >  minE/2570706‑2570841                                                                     |                                                                    tgAGCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCaa                                                                    <  1:3286176/70‑1 (MQ=255) tgAGCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAAt                                                                   >  1:750207/1‑71 (MQ=255)   aGCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATgg                                                                 >  1:4085528/1‑71 (MQ=255)   aGCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATgg                                                                 >  1:3261773/1‑71 (MQ=255)    gCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATg                                                                  <  1:3149440/69‑1 (MQ=255)    gCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGc                                                                <  1:455712/71‑1 (MQ=255)        aaGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCaa                                                                    >  1:3621247/1‑63 (MQ=255)        aaGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTa                                                              <  1:1081723/69‑1 (MQ=255)        aaGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTa                                                              <  1:265145/69‑1 (MQ=255)         agagCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGt                                                           <  1:2388650/71‑1 (MQ=255)          gagCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTAc                                                             >  1:1181721/1‑68 (MQ=255)           agCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTa                                                              >  1:2069328/1‑66 (MQ=255)           agCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                         <  1:823000/71‑1 (MQ=255)             cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                       <  1:2094511/71‑1 (MQ=255)             cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                       <  1:1422282/71‑1 (MQ=255)                  cTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCa                                                                     <  1:2606398/52‑1 (MQ=255)                   ttACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                 >  1:2128142/1‑71 (MQ=255)                    tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAAt                                                                   >  1:3039580/1‑52 (MQ=255)                    tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                 <  1:1388958/70‑1 (MQ=255)                    tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                >  1:2474137/1‑71 (MQ=255)                     aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCaa                                                                    >  1:3830835/1‑50 (MQ=255)                     aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCaa                                                                    >  1:1842152/1‑50 (MQ=255)                     aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCa                                                                     >  1:1364942/1‑49 (MQ=255)                     aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                               >  1:2414018/1‑71 (MQ=255)                     aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                               >  1:1015145/1‑71 (MQ=255)                      cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                              <  1:699814/71‑1 (MQ=255)                      cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                              <  1:160589/71‑1 (MQ=255)                         aaGCAAAATATAACCTTGAGCAGACTGTCATTCACGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                             <  1:478671/69‑1 (MQ=255)                           gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                      <  1:3472115/58‑1 (MQ=255)                           gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCg                                         >  1:3770683/1‑71 (MQ=255)                            cAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGc                                        <  1:607265/71‑1 (MQ=255)                             aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                       <  1:1629912/71‑1 (MQ=255)                              aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                       >  1:3893099/1‑70 (MQ=255)                              aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                       >  1:3217210/1‑70 (MQ=255)                              aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                       >  1:1231582/1‑70 (MQ=255)                               aaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGtga                                                         <  1:2039935/51‑3 (MQ=255)                                atatAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCaa                                                                    >  1:884419/1‑39 (MQ=255)                                atatAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                    >  1:3573855/1‑71 (MQ=255)                                 tataACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                    >  1:746901/1‑70 (MQ=255)                                 tataACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCggg                                   >  1:90012/1‑71 (MQ=255)                                  ataACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCggg                                   >  1:4124072/1‑70 (MQ=255)                                   taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATgg                                                                 >  1:96724/1‑39 (MQ=255)                                   taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGccc                                      <  1:24079/66‑1 (MQ=255)                                   taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                 >  1:145549/1‑71 (MQ=255)                                   taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                 <  1:125495/71‑1 (MQ=255)                                   taACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGt                                  <  1:647673/70‑1 (MQ=255)                                   taACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGt                                  <  1:173734/70‑1 (MQ=255)                                     aCCTTGAGCAGACTGTCATTCGCGCTCCGAGCAATGGCTACGTTACTCAGGTACTGATCC‑CCCAGTTACa                               <  1:1564847/70‑1 (MQ=255)                                     aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                               <  1:364936/71‑1 (MQ=255)                                     aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                               <  1:369893/71‑1 (MQ=255)                                        ttGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                         >  1:52763/1‑42 (MQ=255)                                        ttGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                >  1:91420/1‑67 (MQ=255)                                                aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCa                                     <  1:352150/54‑1 (MQ=255)                                                aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGc                    <  1:3178123/71‑1 (MQ=255)                                                   gTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                             <  1:2200964/43‑1 (MQ=255)                                                    tCATTCGCTCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCtt                 <  1:2425951/70‑1 (MQ=255)                                                    tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                              <  1:1440604/41‑1 (MQ=255)                                                    tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                              <  1:50974/41‑1 (MQ=255)                                                    tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                           >  1:1157155/1‑44 (MQ=255)                                                     cATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                           >  1:781991/1‑43 (MQ=255)                                                     cATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGc               >  1:1136439/1‑71 (MQ=255)                                                      aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATcccc                                        <  1:1082063/45‑3 (MQ=255)                                                      aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGccc                                      <  1:2686883/47‑1 (MQ=255)                                                       ttCGCGCGCCGAGCAATGGCTACGTTACTCAGTTACTGATCCGCCCAGGTa                                 <  1:2577331/51‑1 (MQ=255)                                                       ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                       <  1:3357757/45‑1 (MQ=255)                                                       ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                     >  1:893123/1‑63 (MQ=255)                                                         cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                             <  1:1160454/37‑1 (MQ=255)                                                         cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                             <  1:1250970/37‑1 (MQ=255)                                                         cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                             <  1:4100317/37‑1 (MQ=255)                                                         cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                        >  1:1584515/1‑58 (MQ=255)                                                         cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                       >  1:3002171/1‑59 (MQ=255)                                                         cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt           >  1:3876204/1‑71 (MQ=255)                                                         cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt           >  1:3700257/1‑71 (MQ=255)                                                            gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg        <  1:1270308/71‑1 (MQ=255)                                                                cGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCCTCCg    <  1:2447190/71‑1 (MQ=255)                                                                  aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                 <  1:417223/40‑1 (MQ=255)                                                                  aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                >  1:3563087/1‑41 (MQ=255)                                                                  aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt  <  1:456533/71‑1 (MQ=255)                                                                  aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt  <  1:1065265/71‑1 (MQ=255)                                                                    cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                       >  1:1657286/1‑48 (MQ=255)                                                                    cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                       >  1:381454/1‑48 (MQ=255)                                                                    cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                       >  1:2046087/1‑48 (MQ=255)                                                                    cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                       >  1:3440506/1‑48 (MQ=255)                                                                    cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt           <  1:2007969/60‑1 (MQ=255)                                                                     |                                                                    TGAGCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGT  >  minE/2570706‑2570841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑