Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,570,779 C→T 6.6% G228G (GGC→GGT yibH → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,570,7790CT 6.6% 243.9 / 5.4 75G228G (GGC→GGTyibHhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/38);  new base T (2/3);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

GCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCT  >  minE/2570709‑2570849
                                                                      |                                                                      
gCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGc                                                                        <  1:455712/71‑1 (MQ=255)
    aaGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTa                                                                      <  1:265145/69‑1 (MQ=255)
    aaGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTa                                                                      <  1:1081723/69‑1 (MQ=255)
     agagCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGt                                                                   <  1:2388650/71‑1 (MQ=255)
      gagCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTAc                                                                     >  1:1181721/1‑68 (MQ=255)
       agCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTa                                                                      >  1:2069328/1‑66 (MQ=255)
       agCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                                 <  1:823000/71‑1 (MQ=255)
         cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                               <  1:2094511/71‑1 (MQ=255)
         cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                               <  1:1422282/71‑1 (MQ=255)
               ttACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                         >  1:2128142/1‑71 (MQ=255)
                tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                         <  1:1388958/70‑1 (MQ=255)
                tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                        >  1:2474137/1‑71 (MQ=255)
                 aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                       >  1:1015145/1‑71 (MQ=255)
                 aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                       >  1:2414018/1‑71 (MQ=255)
                  cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                      <  1:699814/71‑1 (MQ=255)
                  cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                      <  1:160589/71‑1 (MQ=255)
                     aaGCAAAATATAACCTTGAGCAGACTGTCATTCACGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                     <  1:478671/69‑1 (MQ=255)
                       gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                              <  1:3472115/58‑1 (MQ=255)
                       gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCg                                                 >  1:3770683/1‑71 (MQ=255)
                        cAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGc                                                <  1:607265/71‑1 (MQ=255)
                         aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                               <  1:1629912/71‑1 (MQ=255)
                          aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                               >  1:3217210/1‑70 (MQ=255)
                          aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                               >  1:1231582/1‑70 (MQ=255)
                          aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                               >  1:3893099/1‑70 (MQ=255)
                           aaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGtga                                                                 <  1:2039935/51‑3 (MQ=255)
                            atatAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                            >  1:3573855/1‑71 (MQ=255)
                             tataACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                            >  1:746901/1‑70 (MQ=255)
                             tataACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCggg                                           >  1:90012/1‑71 (MQ=255)
                              ataACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCggg                                           >  1:4124072/1‑70 (MQ=255)
                               taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGccc                                              <  1:24079/66‑1 (MQ=255)
                               taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                         >  1:145549/1‑71 (MQ=255)
                               taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                         <  1:125495/71‑1 (MQ=255)
                               taACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGt                                          <  1:647673/70‑1 (MQ=255)
                               taACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGt                                          <  1:173734/70‑1 (MQ=255)
                                 aCCTTGAGCAGACTGTCATTCGCGCTCCGAGCAATGGCTACGTTACTCAGGTACTGATCCCCCAGTTACa                                       <  1:1564847/70‑1 (MQ=255)
                                 aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                       <  1:369893/71‑1 (MQ=255)
                                 aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                       <  1:364936/71‑1 (MQ=255)
                                    ttGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                                 >  1:52763/1‑42 (MQ=255)
                                    ttGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                        >  1:91420/1‑67 (MQ=255)
                                            aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCa                                             <  1:352150/54‑1 (MQ=255)
                                            aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGc                            <  1:3178123/71‑1 (MQ=255)
                                               gTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                     <  1:2200964/43‑1 (MQ=255)
                                                tCATTCGCTCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCtt                         <  1:2425951/70‑1 (MQ=255)
                                                tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                      <  1:50974/41‑1 (MQ=255)
                                                tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                      <  1:1440604/41‑1 (MQ=255)
                                                tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                                   >  1:1157155/1‑44 (MQ=255)
                                                 cATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                                   >  1:781991/1‑43 (MQ=255)
                                                 cATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGc                       >  1:1136439/1‑71 (MQ=255)
                                                  aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATcccc                                                <  1:1082063/45‑3 (MQ=255)
                                                  aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGccc                                              <  1:2686883/47‑1 (MQ=255)
                                                   ttCGCGCGCCGAGCAATGGCTACGTTACTCAGTTACTGATCCGCCCAGGTa                                         <  1:2577331/51‑1 (MQ=255)
                                                   ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                               <  1:3357757/45‑1 (MQ=255)
                                                   ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                             >  1:893123/1‑63 (MQ=255)
                                                     cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                     <  1:1160454/37‑1 (MQ=255)
                                                     cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                     <  1:1250970/37‑1 (MQ=255)
                                                     cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                     <  1:4100317/37‑1 (MQ=255)
                                                     cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                >  1:1584515/1‑58 (MQ=255)
                                                     cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                               >  1:3002171/1‑59 (MQ=255)
                                                     cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                   >  1:3700257/1‑71 (MQ=255)
                                                     cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                   >  1:3876204/1‑71 (MQ=255)
                                                        gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                <  1:1270308/71‑1 (MQ=255)
                                                            cGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCCTCCg            <  1:2447190/71‑1 (MQ=255)
                                                              aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                         <  1:417223/40‑1 (MQ=255)
                                                              aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                        >  1:3563087/1‑41 (MQ=255)
                                                              aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt          <  1:1065265/71‑1 (MQ=255)
                                                              aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt          <  1:456533/71‑1 (MQ=255)
                                                                cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                               >  1:2046087/1‑48 (MQ=255)
                                                                cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                               >  1:381454/1‑48 (MQ=255)
                                                                cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                               >  1:1657286/1‑48 (MQ=255)
                                                                cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                               >  1:3440506/1‑48 (MQ=255)
                                                                cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                   <  1:2007969/60‑1 (MQ=255)
                                                                    ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg     >  1:2618532/1‑70 (MQ=255)
                                                                    ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt    >  1:1853236/1‑71 (MQ=255)
                                                                     gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg            <  1:3670160/62‑1 (MQ=255)
                                                                     gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc   >  1:879129/1‑71 (MQ=255)
                                                                     gCTACATTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc   >  1:898074/1‑71 (MQ=255)
                                                                      cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt  <  1:3171041/71‑1 (MQ=255)
                                                                      |                                                                      
GCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCT  >  minE/2570709‑2570849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: