Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,570,782 C→T 6.7% Y229Y (TAC→TAT yibH → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,570,7820CT 6.7% 239.9 / 5.4 74Y229Y (TAC→TATyibHhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/37);  new base T (2/3);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

AGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTC  >  minE/2570714‑2570851
                                                                    |                                                                     
agagCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGt                                                                     <  1:2388650/71‑1 (MQ=255)
 gagCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTAc                                                                       >  1:1181721/1‑68 (MQ=255)
  agCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                                   <  1:823000/71‑1 (MQ=255)
    cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                                 <  1:2094511/71‑1 (MQ=255)
    cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                                 <  1:1422282/71‑1 (MQ=255)
          ttACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                           >  1:2128142/1‑71 (MQ=255)
           tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                           <  1:1388958/70‑1 (MQ=255)
           tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                          >  1:2474137/1‑71 (MQ=255)
            aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                         >  1:2414018/1‑71 (MQ=255)
            aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                         >  1:1015145/1‑71 (MQ=255)
             cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                        <  1:699814/71‑1 (MQ=255)
             cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                        <  1:160589/71‑1 (MQ=255)
                aaGCAAAATATAACCTTGAGCAGACTGTCATTCACGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                       <  1:478671/69‑1 (MQ=255)
                  gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                                <  1:3472115/58‑1 (MQ=255)
                  gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCg                                                   >  1:3770683/1‑71 (MQ=255)
                   cAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGc                                                  <  1:607265/71‑1 (MQ=255)
                    aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                                 <  1:1629912/71‑1 (MQ=255)
                     aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                                 >  1:3217210/1‑70 (MQ=255)
                     aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                                 >  1:3893099/1‑70 (MQ=255)
                     aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                                 >  1:1231582/1‑70 (MQ=255)
                      aaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGtga                                                                   <  1:2039935/51‑3 (MQ=255)
                       atatAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                              >  1:3573855/1‑71 (MQ=255)
                        tataACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                              >  1:746901/1‑70 (MQ=255)
                        tataACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCggg                                             >  1:90012/1‑71 (MQ=255)
                         ataACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCggg                                             >  1:4124072/1‑70 (MQ=255)
                          taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGccc                                                <  1:24079/66‑1 (MQ=255)
                          taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                           >  1:145549/1‑71 (MQ=255)
                          taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                           <  1:125495/71‑1 (MQ=255)
                          taACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGt                                            <  1:173734/70‑1 (MQ=255)
                          taACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGt                                            <  1:647673/70‑1 (MQ=255)
                            aCCTTGAGCAGACTGTCATTCGCGCTCCGAGCAATGGCTACGTTACTCAGGTACTGATCCCCCAGTTACa                                         <  1:1564847/70‑1 (MQ=255)
                            aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                         <  1:364936/71‑1 (MQ=255)
                            aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                         <  1:369893/71‑1 (MQ=255)
                               ttGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                                   >  1:52763/1‑42 (MQ=255)
                               ttGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                          >  1:91420/1‑67 (MQ=255)
                                       aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCa                                               <  1:352150/54‑1 (MQ=255)
                                       aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGc                              <  1:3178123/71‑1 (MQ=255)
                                          gTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                       <  1:2200964/43‑1 (MQ=255)
                                           tCATTCGCTCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCtt                           <  1:2425951/70‑1 (MQ=255)
                                           tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                        <  1:50974/41‑1 (MQ=255)
                                           tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                        <  1:1440604/41‑1 (MQ=255)
                                           tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                                     >  1:1157155/1‑44 (MQ=255)
                                            cATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                                     >  1:781991/1‑43 (MQ=255)
                                            cATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGc                         >  1:1136439/1‑71 (MQ=255)
                                             aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATcccc                                                  <  1:1082063/45‑3 (MQ=255)
                                             aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGccc                                                <  1:2686883/47‑1 (MQ=255)
                                              ttCGCGCGCCGAGCAATGGCTACGTTACTCAGTTACTGATCCGCCCAGGTa                                           <  1:2577331/51‑1 (MQ=255)
                                              ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                                 <  1:3357757/45‑1 (MQ=255)
                                              ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                               >  1:893123/1‑63 (MQ=255)
                                                cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                       <  1:1250970/37‑1 (MQ=255)
                                                cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                       <  1:1160454/37‑1 (MQ=255)
                                                cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                       <  1:4100317/37‑1 (MQ=255)
                                                cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                  >  1:1584515/1‑58 (MQ=255)
                                                cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                 >  1:3002171/1‑59 (MQ=255)
                                                cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                     >  1:3700257/1‑71 (MQ=255)
                                                cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                     >  1:3876204/1‑71 (MQ=255)
                                                   gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                  <  1:1270308/71‑1 (MQ=255)
                                                       cGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCCTCCg              <  1:2447190/71‑1 (MQ=255)
                                                         aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                           <  1:417223/40‑1 (MQ=255)
                                                         aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                          >  1:3563087/1‑41 (MQ=255)
                                                         aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt            <  1:1065265/71‑1 (MQ=255)
                                                         aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt            <  1:456533/71‑1 (MQ=255)
                                                           cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                 >  1:381454/1‑48 (MQ=255)
                                                           cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                 >  1:1657286/1‑48 (MQ=255)
                                                           cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                 >  1:2046087/1‑48 (MQ=255)
                                                           cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                 >  1:3440506/1‑48 (MQ=255)
                                                           cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                     <  1:2007969/60‑1 (MQ=255)
                                                               ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg       >  1:2618532/1‑70 (MQ=255)
                                                               ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt      >  1:1853236/1‑71 (MQ=255)
                                                                gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg              <  1:3670160/62‑1 (MQ=255)
                                                                gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc     >  1:879129/1‑71 (MQ=255)
                                                                gCTACATTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc     >  1:898074/1‑71 (MQ=255)
                                                                 cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt    <  1:3171041/71‑1 (MQ=255)
                                                                   aCGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                    >  1:418328/1‑37 (MQ=255)
                                                                   aCGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                    >  1:3672401/1‑37 (MQ=255)
                                                                    cGTTACTCATGTACTGATCCGCCCAGTTACATACGCAGCTGCCTTGCCGCt                     <  1:2603132/51‑1 (MQ=255)
                                                                    cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc  <  1:3110670/70‑1 (MQ=255)
                                                                    |                                                                     
AGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTC  >  minE/2570714‑2570851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: