Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,570,785	T→G	5.4%	V230V (GTT→GTG)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,570,785	0	T	G	5.4%	195.8 / 6.2	74	V230V (GTT→GTG)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/38);  new base G (2/2);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

AGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTC  >  minE/2570716‑2570851                                                                      |                                                                   agCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                                   <  1:823000/71‑1 (MQ=255)   cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                                 <  1:2094511/71‑1 (MQ=255)   cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                                 <  1:1422282/71‑1 (MQ=255)         ttACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                           >  1:2128142/1‑71 (MQ=255)          tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                           <  1:1388958/70‑1 (MQ=255)          tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                          >  1:2474137/1‑71 (MQ=255)           aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                         >  1:1015145/1‑71 (MQ=255)           aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                         >  1:2414018/1‑71 (MQ=255)            cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                        <  1:160589/71‑1 (MQ=255)            cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                        <  1:699814/71‑1 (MQ=255)               aaGCAAAATATAACCTTGAGCAGACTGTCATTCACGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                       <  1:478671/69‑1 (MQ=255)                 gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                                <  1:3472115/58‑1 (MQ=255)                 gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCg                                                   >  1:3770683/1‑71 (MQ=255)                  cAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGc                                                  <  1:607265/71‑1 (MQ=255)                   aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                                 <  1:1629912/71‑1 (MQ=255)                    aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                                 >  1:3217210/1‑70 (MQ=255)                    aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                                 >  1:1231582/1‑70 (MQ=255)                    aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                                 >  1:3893099/1‑70 (MQ=255)                      atatAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                              >  1:3573855/1‑71 (MQ=255)                       tataACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                              >  1:746901/1‑70 (MQ=255)                       tataACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCggg                                             >  1:90012/1‑71 (MQ=255)                        ataACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCggg                                             >  1:4124072/1‑70 (MQ=255)                         taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGccc                                                <  1:24079/66‑1 (MQ=255)                         taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                           <  1:125495/71‑1 (MQ=255)                         taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                           >  1:145549/1‑71 (MQ=255)                         taACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGt                                            <  1:173734/70‑1 (MQ=255)                         taACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGt                                            <  1:647673/70‑1 (MQ=255)                           aCCTTGAGCAGACTGTCATTCGCGCTCCGAGCAATGGCTACGTTACTCAGGTACTGATCC‑CCCAGTTACa                                         <  1:1564847/70‑1 (MQ=255)                           aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                         <  1:369893/71‑1 (MQ=255)                           aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                         <  1:364936/71‑1 (MQ=255)                              ttGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                                   >  1:52763/1‑42 (MQ=255)                              ttGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                          >  1:91420/1‑67 (MQ=255)                                      aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCa                                               <  1:352150/54‑1 (MQ=255)                                      aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGc                              <  1:3178123/71‑1 (MQ=255)                                         gTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                       <  1:2200964/43‑1 (MQ=255)                                          tCATTCGCTCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCtt                           <  1:2425951/70‑1 (MQ=255)                                          tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                        <  1:1440604/41‑1 (MQ=255)                                          tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTg                                                        <  1:50974/41‑1 (MQ=255)                                          tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                                     >  1:1157155/1‑44 (MQ=255)                                           cATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                                     >  1:781991/1‑43 (MQ=255)                                           cATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGc                         >  1:1136439/1‑71 (MQ=255)                                            aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATcccc                                                  <  1:1082063/45‑3 (MQ=255)                                            aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGccc                                                <  1:2686883/47‑1 (MQ=255)                                             ttCGCGCGCCGAGCAATGGCTACGTTACTCAGTTACTGATCCGCCCAGGTa                                           <  1:2577331/51‑1 (MQ=255)                                             ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGcc                                                 <  1:3357757/45‑1 (MQ=255)                                             ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                               >  1:893123/1‑63 (MQ=255)                                               cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                       <  1:4100317/37‑1 (MQ=255)                                               cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                       <  1:1250970/37‑1 (MQ=255)                                               cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                       <  1:1160454/37‑1 (MQ=255)                                               cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                  >  1:1584515/1‑58 (MQ=255)                                               cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                 >  1:3002171/1‑59 (MQ=255)                                               cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                     >  1:3700257/1‑71 (MQ=255)                                               cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                     >  1:3876204/1‑71 (MQ=255)                                                  gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                  <  1:1270308/71‑1 (MQ=255)                                                      cGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCCTCCg              <  1:2447190/71‑1 (MQ=255)                                                        aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                           <  1:417223/40‑1 (MQ=255)                                                        aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                          >  1:3563087/1‑41 (MQ=255)                                                        aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt            <  1:456533/71‑1 (MQ=255)                                                        aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt            <  1:1065265/71‑1 (MQ=255)                                                          cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                 >  1:381454/1‑48 (MQ=255)                                                          cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                 >  1:1657286/1‑48 (MQ=255)                                                          cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                 >  1:3440506/1‑48 (MQ=255)                                                          cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                 >  1:2046087/1‑48 (MQ=255)                                                          cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                     <  1:2007969/60‑1 (MQ=255)                                                              ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg       >  1:2618532/1‑70 (MQ=255)                                                              ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt      >  1:1853236/1‑71 (MQ=255)                                                               gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg              <  1:3670160/62‑1 (MQ=255)                                                               gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc     >  1:879129/1‑71 (MQ=255)                                                               gCTACATTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc     >  1:898074/1‑71 (MQ=255)                                                                cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt    <  1:3171041/71‑1 (MQ=255)                                                                  aCGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                    >  1:418328/1‑37 (MQ=255)                                                                  aCGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                    >  1:3672401/1‑37 (MQ=255)                                                                   cGTTACTCATGTACTGATCCGCCCAGTTACATACGCAGCTGCCTTGCCGCt                     <  1:2603132/51‑1 (MQ=255)                                                                   cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc  <  1:3110670/70‑1 (MQ=255)                                                                      tACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg              >  1:1107052/1‑55 (MQ=255)                                                                      tACTCAAGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa          >  1:3583/1‑59 (MQ=255)                                                                      |                                                                   AGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTC  >  minE/2570716‑2570851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑