| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,571,166 | T→C | 13.8% | V357V (GTT→GTC) | yibH → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,571,166 | 0 | T | C | 13.8% | 336.3 / 42.0 | 130 | V357V (GTT→GTC) | yibH | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (47/65); new base C (12/6); total (59/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01 | |||||||||||
Only 100 of 140 total aligned reads displayed.
ATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGG > minE/2571096‑2571235 | atgatATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGtt < 1:46342/71‑1 (MQ=255) atatCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTc < 1:3890893/70‑1 (MQ=255) atatCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCg < 1:2177103/71‑1 (MQ=255) tatCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTc > 1:3011480/1‑69 (MQ=255) tCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGttt < 1:1774778/66‑1 (MQ=255) gATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGa < 1:1986810/70‑1 (MQ=255) tGCCTTACCCGACGGCATCTACGCACAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGc > 1:3582736/1‑71 (MQ=255) gctttACCAGACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGtctc < 1:2136055/59‑1 (MQ=255) ccTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCg > 1:3995500/1‑70 (MQ=255) cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCgg > 1:2943204/1‑70 (MQ=255) cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:604/71‑1 (MQ=255) cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:2756688/71‑1 (MQ=255) cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:1553612/71‑1 (MQ=255) ttACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa > 1:491004/1‑70 (MQ=255) ttACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa > 1:2457986/1‑70 (MQ=255) tACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCgg < 1:95550/60‑1 (MQ=255) tACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCgg < 1:105244/60‑1 (MQ=255) tACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCgg < 1:128929/60‑1 (MQ=255) tACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa > 1:3770779/1‑69 (MQ=255) aCCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGaa < 1:3116470/69‑1 (MQ=255) aCGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgcta > 1:230951/1‑70 (MQ=255) aCGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCgg < 1:3999344/54‑1 (MQ=255) aCGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:717041/63‑1 (MQ=255) cGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgctat < 1:1012803/71‑3 (MQ=255) tttACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCaaa > 1:248902/3‑59 (MQ=255) tCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTc < 1:2887505/46‑1 (MQ=255) tCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGaa < 1:1554937/58‑1 (MQ=255) tCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:1714441/57‑1 (MQ=255) tCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg > 1:1434260/1‑70 (MQ=255) cTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa > 1:2402462/1‑71 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTc > 1:3264540/1‑44 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTc > 1:3959796/1‑44 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCgg > 1:1451582/1‑46 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCgg > 1:1949026/1‑46 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCgg > 1:1605564/1‑46 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt > 1:1932371/1‑47 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGaaa > 1:119096/1‑57 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAAt > 1:133312/1‑71 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAAt > 1:111777/1‑71 (MQ=255) aCGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTc > 1:1845410/1‑43 (MQ=255) aCGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt < 1:292240/46‑1 (MQ=255) cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa < 1:3668258/71‑1 (MQ=255) cAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTc < 1:1743392/38‑1 (MQ=255) ggTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt < 1:3194704/39‑1 (MQ=255) ggTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTaa < 1:2728731/59‑1 (MQ=255) gTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTg > 1:210843/1‑71 (MQ=255) gTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGtg < 1:3857489/51‑1 (MQ=255) ggCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa > 1:545315/1‑71 (MQ=255) gCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa < 1:952344/70‑1 (MQ=255) cGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATg > 1:2814993/1‑71 (MQ=255) ggTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGc < 1:3009714/71‑1 (MQ=255) ggTTTACTCCGACCATTTCAGCCATGTTTCGGTGa > 1:2255146/1‑35 (MQ=255) ggTTTACTCCGACCATTTCAGCCATGTTACGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg < 1:3312450/66‑1 (MQ=255) gTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCa < 1:3919056/71‑1 (MQ=255) tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTaa < 1:1068004/51‑1 (MQ=255) tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTaa < 1:4142337/51‑1 (MQ=255) ttACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCaaa > 1:1607759/1‑40 (MQ=255) ttACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgcta > 1:480131/1‑45 (MQ=255) ttACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgcta > 1:1349466/1‑45 (MQ=255) ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa > 1:3380717/1‑56 (MQ=255) ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt > 1:3169414/1‑70 (MQ=255) tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGc > 1:2947690/1‑60 (MQ=255) tACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAtt < 1:230781/70‑1 (MQ=255) aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTa < 1:253048/70‑1 (MQ=255) aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTa < 1:248070/70‑1 (MQ=255) cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg > 1:2545533/1‑45 (MQ=255) cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa > 1:2593160/1‑50 (MQ=255) cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt > 1:3403223/1‑56 (MQ=255) cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCCGCTAAGAATGACCAGCTGGATGCATTATCtt > 1:2423530/1‑70 (MQ=255) cATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCa > 1:930626/1‑59 (MQ=255) cATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCa > 1:3276909/1‑59 (MQ=255) aTTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa < 1:1667984/67‑1 (MQ=255) tttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg < 1:2863215/39‑1 (MQ=255) tttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTgg < 1:679027/71‑1 (MQ=255) tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGa < 1:2342399/70‑1 (MQ=255) aGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCa < 1:2335195/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:44487/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:630160/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:3093849/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:795084/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:423590/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:925931/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:3976246/71‑1 (MQ=255) cATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCtt > 1:4082641/1‑57 (MQ=255) cATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTg < 1:3087011/71‑1 (MQ=255) cATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTg < 1:775427/71‑1 (MQ=255) cATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTg < 1:3683127/71‑1 (MQ=255) aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg < 1:2191325/43‑1 (MQ=255) aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt > 1:3613364/1‑69 (MQ=255) tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg < 1:2223298/42‑1 (MQ=255) tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg < 1:1544684/42‑1 (MQ=255) tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg < 1:419792/42‑1 (MQ=255) tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCa < 1:824364/66‑1 (MQ=255) tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt < 1:2066690/67‑1 (MQ=255) tGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCttt < 1:2338841/56‑1 (MQ=255) gTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGc < 1:623439/39‑1 (MQ=255) tttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt > 1:485237/1‑39 (MQ=255) tttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa > 1:2596392/1‑42 (MQ=255) tttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt < 1:2604808/58‑1 (MQ=255) tttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggg > 1:4022860/1‑71 (MQ=255) | ATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGG > minE/2571096‑2571235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |