| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,571,178 | G→C | 14.5% | R361R (CGG→CGC) | yibH → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,571,178 | 0 | G | C | 14.5% | 331.3 / 41.8 | 131 | R361R (CGG→CGC) | yibH | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (49/63); new base C (14/5); total (63/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.39e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01 | |||||||||||
Only 100 of 137 total aligned reads displayed.
CTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGAT > minE/2571109‑2571248 | cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:126297/71‑1 (MQ=255)cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:604/71‑1 (MQ=255)cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:1553612/71‑1 (MQ=255) ttACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa > 1:2457986/1‑70 (MQ=255) ttACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa > 1:491004/1‑70 (MQ=255) aCCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGaa < 1:3116470/69‑1 (MQ=255) aCGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgcta > 1:230951/1‑70 (MQ=255) cGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgctat < 1:1012803/71‑3 (MQ=255) tttACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCaaa > 1:248902/3‑59 (MQ=255) tCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGaa < 1:1554937/58‑1 (MQ=255) tCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:1714441/57‑1 (MQ=255) tCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:416130/57‑1 (MQ=255) tCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg > 1:1434260/1‑70 (MQ=255) cTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa > 1:2402462/1‑71 (MQ=255) cTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa > 1:3994567/1‑70 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGaaa > 1:119096/1‑57 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAAt > 1:133312/1‑71 (MQ=255) tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAAt > 1:3220899/1‑71 (MQ=255) cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAAt < 1:4080024/69‑1 (MQ=255) cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa < 1:3668258/71‑1 (MQ=255) ggTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTaa < 1:2728731/59‑1 (MQ=255) gTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTg > 1:1095014/1‑71 (MQ=255) gTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTg > 1:210843/1‑71 (MQ=255) gTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:2594655/46‑1 (MQ=255) gTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGtg < 1:3857489/51‑1 (MQ=255) tGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa < 1:2116866/45‑1 (MQ=255) ggCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCgg > 1:938061/1‑43 (MQ=255) ggCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa > 1:545315/1‑71 (MQ=255) gCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAAt > 1:1617535/1‑59 (MQ=255) gCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa < 1:952344/70‑1 (MQ=255) cGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATg > 1:2814993/1‑71 (MQ=255) cGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATg < 1:239614/71‑1 (MQ=255) ggTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGc < 1:3009714/71‑1 (MQ=255) ggTTTACTCCGACCATTTCAGCCATGTTACGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg < 1:3312450/66‑1 (MQ=255) gTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCa < 1:3919056/71‑1 (MQ=255) tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTaa < 1:1068004/51‑1 (MQ=255) ttACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCaaa > 1:1607759/1‑40 (MQ=255) ttACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgcta > 1:480131/1‑45 (MQ=255) ttACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgcta > 1:1349466/1‑45 (MQ=255) ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa > 1:3380717/1‑56 (MQ=255) ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt > 1:2338357/1‑70 (MQ=255) tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGc > 1:2947690/1‑60 (MQ=255) tACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt > 1:3680293/1‑69 (MQ=255) tACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTa > 1:1855529/1‑71 (MQ=255) aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTa < 1:253048/70‑1 (MQ=255) cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg > 1:2717872/1‑45 (MQ=255) cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa > 1:2593160/1‑50 (MQ=255) cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt > 1:3403223/1‑56 (MQ=255) cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa > 1:251483/1‑43 (MQ=255) cATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCa > 1:3276909/1‑59 (MQ=255) aTTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa < 1:1667984/67‑1 (MQ=255) tttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg < 1:2863215/39‑1 (MQ=255) tttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATg < 1:2207404/43‑1 (MQ=255) tttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTgg < 1:679027/71‑1 (MQ=255) tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGa < 1:2342399/70‑1 (MQ=255) tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGAt > 1:746082/1‑71 (MQ=255) aGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCa < 1:2335195/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa < 1:457302/60‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:795084/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:3976246/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:630160/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:423590/71‑1 (MQ=255) ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt < 1:925931/71‑1 (MQ=255) cATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCtt > 1:4082641/1‑57 (MQ=255) cATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTg < 1:3087011/71‑1 (MQ=255) cATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTg < 1:3683127/71‑1 (MQ=255) cATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTg < 1:775427/71‑1 (MQ=255) aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt > 1:3613364/1‑69 (MQ=255) aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt > 1:2265557/1‑69 (MQ=255) tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg < 1:2223298/42‑1 (MQ=255) tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg < 1:419792/42‑1 (MQ=255) tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg < 1:295266/42‑1 (MQ=255) tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg < 1:1544684/42‑1 (MQ=255) tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCa < 1:824364/66‑1 (MQ=255) tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt < 1:2066690/67‑1 (MQ=255) tGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCttt < 1:2911709/56‑1 (MQ=255) gTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGc < 1:623439/39‑1 (MQ=255) tttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt > 1:485237/1‑39 (MQ=255) tttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa > 1:2596392/1‑42 (MQ=255) tttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa > 1:148076/1‑42 (MQ=255) tctCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAt > 1:2445816/3‑56 (MQ=255) tctCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAt > 1:1880178/3‑56 (MQ=255) tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa > 1:899206/1‑40 (MQ=255) tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTa > 1:3582595/1‑47 (MQ=255) tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTa > 1:3041325/1‑47 (MQ=255) tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt > 1:3999649/1‑64 (MQ=255) tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt > 1:3111456/1‑63 (MQ=255) tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg < 1:3833739/70‑1 (MQ=255) tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg < 1:594621/70‑1 (MQ=255) tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg < 1:3936350/70‑1 (MQ=255) tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg < 1:3462416/70‑1 (MQ=255) tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggg > 1:1463137/1‑69 (MQ=255) tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTga > 1:1228556/1‑66 (MQ=255) tCGGAGATGCGGAAAGTGCTGCTAAGAATGACCAg > 1:2288865/1‑35 (MQ=255) cGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggg < 1:1839896/70‑1 (MQ=255) ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa > 1:3888278/1‑38 (MQ=255) tGATGCGGAAAGTGCTGCTAAGAATGACCATCTGGATGCATTATCTTTATTTTTATCa < 1:2245708/58‑1 (MQ=255) tGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgag < 1:409267/60‑1 (MQ=255) gCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATc > 1:1592872/1‑41 (MQ=255) gAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGAt > 1:295657/1‑71 (MQ=255) | CTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGAT > minE/2571109‑2571248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |