| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,487 | A→G | 21.0% | intergenic (+1255/‑524) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,487 | 0 | A | G | 21.0% | 155.8 / 38.8 | 76 | intergenic (+1255/‑524) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (33/27); new base G (11/5); total (44/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATGCCGTCAGGTTTTCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGG > minE/2572417‑2572556 | tATGCCGTCATGTTTTCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTa < 1:1063346/71‑1 (MQ=255) tGCCGTCAGGTTTTCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGc > 1:3826341/1‑71 (MQ=255) ccGTCAGGTTTTCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTGGCCa > 1:541424/1‑71 (MQ=255) ccGTCAGGTTTTCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCa > 1:3390577/1‑71 (MQ=255) aGGTTTTCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTGGCCATCTAt > 1:3443897/1‑71 (MQ=255) aGGTTTTCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTGGCCATCTAt > 1:500533/1‑71 (MQ=255) aGGTTTTCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTAt > 1:967463/1‑71 (MQ=255) aGGTTTTCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTAt > 1:457527/1‑71 (MQ=255) ttttCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCAt < 1:2663418/64‑1 (MQ=255) tttCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCAt > 1:144531/1‑63 (MQ=255) tttCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTta > 1:2550139/1‑70 (MQ=255) ttCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTGGCCATCTATTtat > 1:28089/1‑70 (MQ=255) tCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTtata < 1:497102/70‑1 (MQ=255) ttgcttgcTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATaaa < 1:1702612/68‑1 (MQ=255) ttgcttgcTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATaaa < 1:4058463/68‑1 (MQ=255) ttgcttgcTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAAt < 1:2029191/69‑1 (MQ=255) gcttgctCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTta > 1:51880/1‑62 (MQ=255) gcttgctCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAAtgtg < 1:279889/70‑1 (MQ=255) cttgctCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATgtgt < 1:3273523/70‑1 (MQ=255) gctCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTGGCCATCTATTTATAAATGTGTACa < 1:1365896/70‑1 (MQ=255) gctCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAAtgtg > 1:3155666/1‑66 (MQ=255) ctCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACaa < 1:1898797/70‑1 (MQ=255) ctCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACa < 1:3100369/69‑1 (MQ=255) gggTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTGGCCATCTATTTATAAATGTGTACAACGGGGa > 1:1650687/1‑71 (MQ=255) ggTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTGGCCATCTATTTATAAATGTGTACAACGGGGat < 1:1227866/71‑1 (MQ=255) ggTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACa < 1:3105336/63‑1 (MQ=255) gTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACggg > 1:1870444/1‑67 (MQ=255) gTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGat > 1:1648688/1‑70 (MQ=255) tCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCa < 1:2128680/41‑1 (MQ=255) tCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATc < 1:1329623/43‑1 (MQ=255) cATTAACATTCCAACTCCATAGGCCTTGAGGTATTGGCCATCTATTTATAAATGTGTACAACGGGGattat > 1:3736116/1‑71 (MQ=255) aTTAACATTCCAACTCCATAGGCCTTGAGGTATTGGCCATCTATTTATAAATGTGTACAACGGGGattat > 1:2594531/1‑70 (MQ=255) aTTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGattat > 1:2412843/1‑70 (MQ=255) tAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTAc > 1:3733741/1‑56 (MQ=255) tAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTAc > 1:136299/1‑56 (MQ=255) tAACATTACAACTCCATAGGCCTTGAGGTATTGGCCATCTATTTATAAATGTGTACAACGGGGATTAtttt > 1:1178655/1‑71 (MQ=255) aaCATTCCAACTCCATAGGCCTTGAGGTATTAGCCa > 1:889813/1‑36 (MQ=255) aaCATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAAtgtg > 1:694728/1‑52 (MQ=255) aaCATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAAtgtg > 1:1434506/1‑52 (MQ=255) aaCATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTAtttt > 1:2902021/1‑70 (MQ=255) aaCATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTAtttt > 1:2261337/1‑70 (MQ=255) aaCATTCCAACTCCATAGGCCTTCAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTAtttt > 1:3259170/1‑70 (MQ=255) aCATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTa < 1:2776941/71‑1 (MQ=255) aCATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTa > 1:1900368/1‑71 (MQ=255) aCATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTa < 1:2001656/71‑1 (MQ=255) aTTCCAACTCCATAGGCCTTGAGGTATTGGCCATCTATTTATAAATGTGTACAACGGGGattat < 1:304953/64‑1 (MQ=255) aTTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTa < 1:1468459/69‑1 (MQ=255) ttCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACgg < 1:2558635/56‑1 (MQ=255) ttCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGattatt < 1:3887134/64‑1 (MQ=255) tCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACAtt > 1:3920581/1‑71 (MQ=255) aaCTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACAtt > 1:3035093/1‑68 (MQ=255) aCTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTAc > 1:3159552/1‑64 (MQ=255) cATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCatat < 1:371115/69‑1 (MQ=255) cATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCAtatta > 1:3230619/1‑71 (MQ=255) aTAGGCCTTGAGGTATTAGCCATCTATTTATAAAtgt < 1:2038569/37‑1 (MQ=255) aTAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTAttt > 1:3877312/1‑55 (MQ=255) tAGGCCTTGAGGTATTGGCCATCTATTTATAAATGTGTACAACGGGGatt < 1:1146423/50‑1 (MQ=255) tAGGCCTTGAGGTATTGGCCATCTATTTATAAATGTGTACAACGGGGatt < 1:917084/50‑1 (MQ=255) tAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATattatt < 1:1596125/71‑1 (MQ=255) ggCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTAttttt < 1:2258627/54‑1 (MQ=255) gCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTAc > 1:14862/1‑70 (MQ=255) ccTTGAGGTATTGGCCATCTATTTATAAATGTGTTCAACggg > 1:598038/1‑42 (MQ=255) ccTTGAGGTATTGGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACa > 1:397725/1‑55 (MQ=255) ccTTGAGGTATTGGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACa > 1:2938181/1‑55 (MQ=255) cTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTAc > 1:3161938/1‑68 (MQ=255) tGAGGTATTAGCCATCTATTTATAAATGTGTACAACggg < 1:3199442/39‑1 (MQ=255) gAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTa > 1:1127745/1‑49 (MQ=255) gAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACa > 1:1267680/1‑51 (MQ=255) gAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAg > 1:322481/1‑71 (MQ=255) gAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAg > 1:947068/1‑71 (MQ=255) aGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGc < 1:1632817/71‑1 (MQ=255) ggTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCg < 1:2715020/71‑1 (MQ=255) gTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGa > 1:517454/1‑71 (MQ=255) aTTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACt > 1:1861309/1‑71 (MQ=255) ttAGCCATCTATTTATAAATGTGTACAACGGGGATTAttt < 1:3937994/40‑1 (MQ=255) ttAGCCATCTATTTATAAATGTGTACAACGGGGATTAttt < 1:3933383/40‑1 (MQ=255) ttAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTAc < 1:2808748/44‑1 (MQ=255) aGCCATCTATTTATAAATGTGTACAACGGGGATTAtttt > 1:1138400/1‑39 (MQ=255) aGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTgg > 1:175490/1‑70 (MQ=255) aGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTgg > 1:3314929/1‑70 (MQ=255) | TATGCCGTCAGGTTTTCTATTGCTTGCTCCCGGGTCATTAACATTCCAACTCCATAGGCCTTGAGGTATTAGCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGG > minE/2572417‑2572556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |