| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,705 | C→T | 18.4% | intergenic (+1473/‑306) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,705 | 0 | C | T | 18.4% | 271.6 / 49.3 | 114 | intergenic (+1473/‑306) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (40/53); new base T (14/7); total (54/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
TAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGT > minE/2572635‑2572775 | taAACAGGTGGCTAATCCCAAGGGGATCTATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCac > 1:1816853/1‑71 (MQ=255) cAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacctac < 1:2688241/71‑1 (MQ=255) aaTCCCAAGGTGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGcc > 1:1612121/1‑71 (MQ=255) aaTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacctac < 1:3029301/62‑1 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCtt > 1:2499087/1‑63 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGccc > 1:1021027/1‑70 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGccc > 1:1269454/1‑70 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:2772588/71‑1 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:2158085/71‑1 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAAATCAACCTACCTTCCAGCCCa < 1:3674630/71‑1 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGGCCAAt > 1:3284214/1‑71 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCaa > 1:1063436/1‑70 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:2753554/1‑71 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:933206/68‑1 (MQ=255) aaGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacctacct < 1:1733727/58‑1 (MQ=255) aaGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:2666170/1‑71 (MQ=255) ggggATCGATTCCAGAAATCGGGTGCAACGGATACTGATAAAAATTCCacc < 1:3147181/51‑1 (MQ=255) gggATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAg > 1:1632056/1‑71 (MQ=255) tCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTg < 1:3901341/71‑1 (MQ=255) tCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTg < 1:734034/71‑1 (MQ=255) cGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCt > 1:3383275/1‑69 (MQ=255) gATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa > 1:716211/1‑53 (MQ=255) gATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAg > 1:1264899/1‑71 (MQ=255) aTTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgt < 1:3702400/71‑1 (MQ=255) ttCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGccc < 1:874905/55‑1 (MQ=255) tCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtg < 1:605181/70‑1 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:3787628/70‑1 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:390349/71‑1 (MQ=255) aGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt < 1:1872515/54‑1 (MQ=255) aGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtat < 1:914487/71‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtata > 1:3445080/1‑71 (MQ=255) aaaTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa > 1:2142621/1‑45 (MQ=255) aaTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:1237055/65‑1 (MQ=255) cGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGccc > 1:3972475/1‑45 (MQ=255) cGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGAta > 1:1617017/1‑64 (MQ=255) gggTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:702095/1‑47 (MQ=255) gggTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGccc > 1:3634672/1‑71 (MQ=255) gTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:2946995/1‑47 (MQ=255) gTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCct < 1:782865/71‑1 (MQ=255) ttCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCt < 1:1275520/52‑1 (MQ=255) ttCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:3534905/58‑1 (MQ=255) cAACGGATACTGATACATATTCCATCCACCTCTCAACCCAATCGGGTCCGGGGTGATATACCGCCCctgc < 1:3681761/70‑1 (MQ=255) cAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGccccag > 1:1241624/1‑67 (MQ=255) cAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgct > 1:3689940/1‑71 (MQ=255) cAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgct > 1:3488594/1‑71 (MQ=255) aaCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCcagca < 1:3263023/70‑2 (MQ=255) aaCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCcagca < 1:3629112/70‑2 (MQ=255) aCGGATACTGATACATATTCCATCCACCTCTCAACCCAATCgg > 1:2531630/1‑43 (MQ=255) aCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt < 1:3335460/40‑1 (MQ=255) cGGATACTGATAAAAATTCCACCTCCCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCg > 1:2784264/1‑71 (MQ=255) cGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCg > 1:1169944/1‑71 (MQ=255) cGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCg < 1:1144066/71‑1 (MQ=255) ggATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:1446459/1‑71 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGccc > 1:1016167/1‑35 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:2394455/1‑40 (MQ=255) gATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCg < 1:545833/39‑1 (MQ=25) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtg < 1:3245108/49‑1 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:2921150/1‑52 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt > 1:532667/1‑70 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt > 1:1447469/1‑70 (MQ=255) tACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:86929/1‑51 (MQ=255) tACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGtt > 1:1461899/1‑70 (MQ=255) aCTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGa < 1:1864114/38‑1 (MQ=12) cTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATAc > 1:2670562/1‑52 (MQ=255) cTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAAt < 1:3380212/71‑1 (MQ=255) cTGATAAAAATCCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATAc > 1:2403125/1‑52 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:2543605/48‑1 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGtt < 1:3353523/67‑1 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAgtgat > 1:3220834/1‑44 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATAc > 1:1525899/1‑50 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATAc > 1:346663/1‑50 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATAc > 1:2645672/1‑50 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtgat < 1:3902469/46‑3 (MQ=255) gATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtata > 1:2062969/1‑49 (MQ=255) gATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGtt < 1:504292/66‑1 (MQ=255) aTAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtgat > 1:4064961/1‑43 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGATTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:2329000/71‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:2963637/46‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg < 1:156782/60‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg < 1:3080931/60‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt < 1:2890094/64‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:3621980/71‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGt < 1:3625784/70‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGt < 1:2755390/70‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCT‑AGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:3137623/70‑1 (MQ=255) tAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCct > 1:2141774/1‑55 (MQ=255) tAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTa < 1:3945637/71‑1 (MQ=255) aaaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAg > 1:3628962/1‑39 (MQ=255) aaaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt > 1:763830/1‑62 (MQ=255) aaaaTTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGAta > 1:1564126/1‑43 (MQ=255) aaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg < 1:610657/57‑1 (MQ=255) aaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGa > 1:2694211/1‑71 (MQ=255) ttCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGata < 1:1348121/71‑1 (MQ=255) ttCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGata < 1:351529/71‑1 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataa > 1:429961/1‑70 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataata < 1:612605/71‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:686262/70‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:691148/70‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:1454696/70‑1 (MQ=255) cctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt < 1:723006/71‑1 (MQ=255) | TAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGT > minE/2572635‑2572775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |