| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,713 | C→T | 16.4% | intergenic (+1481/‑298) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,713 | 0 | C | T | 16.4% | 329.7 / 47.5 | 128 | intergenic (+1481/‑298) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (48/59); new base T (14/7); total (62/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.41e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
AATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGT > minE/2572648‑2572781 | aaTCCCAAGGTGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGcc > 1:1612121/1‑71 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGccc > 1:1021027/1‑70 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGccc > 1:1269454/1‑70 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:2772588/71‑1 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:2158085/71‑1 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGGCCAAt > 1:3284214/1‑71 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCaa > 1:1063436/1‑70 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:2753554/1‑71 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:933206/68‑1 (MQ=255) aaGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:2666170/1‑71 (MQ=255) gggATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAg > 1:1632056/1‑71 (MQ=255) tCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCt > 1:694563/1‑70 (MQ=255) tCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTg < 1:734034/71‑1 (MQ=255) tCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTg < 1:4001960/71‑1 (MQ=255) tCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTg < 1:3901341/71‑1 (MQ=255) cGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCt > 1:3383275/1‑69 (MQ=255) gATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa > 1:716211/1‑53 (MQ=255) gATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAg > 1:1264899/1‑71 (MQ=255) aTTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgt < 1:3702400/71‑1 (MQ=255) ttCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGccc < 1:874905/55‑1 (MQ=255) tCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtg < 1:605181/70‑1 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:779791/70‑1 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:3787628/70‑1 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:390349/71‑1 (MQ=255) aGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtat < 1:131062/71‑1 (MQ=255) aGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtat < 1:914487/71‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtata > 1:3445080/1‑71 (MQ=255) tCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:1624669/1‑49 (MQ=255) tCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:1477177/1‑65 (MQ=255) cGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGccc > 1:3972475/1‑45 (MQ=255) gggTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGccc > 1:3634672/1‑71 (MQ=255) ggattCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGATATATCGccc > 1:3595513/4‑71 (MQ=255) gTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCct < 1:782865/71‑1 (MQ=255) cAACGGATACTGATACATATTCCATCCACCTCTCAACCCAATCGGGTCCGGGGTGATATACCGCCCctgc < 1:3681761/70‑1 (MQ=255) cAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGccccag > 1:1241624/1‑67 (MQ=255) cAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgct > 1:3689940/1‑71 (MQ=255) cAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgct > 1:3488594/1‑71 (MQ=255) aaCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCcagca < 1:3629112/70‑2 (MQ=255) aCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt < 1:3335460/40‑1 (MQ=255) aCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGtt < 1:2544727/55‑1 (MQ=255) cGGATACTGATAAAAATTCCACCTCCCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCg > 1:2784264/1‑71 (MQ=255) cGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCg > 1:1169944/1‑71 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGccc > 1:1016167/1‑35 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:2394455/1‑40 (MQ=255) gATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCg < 1:545833/39‑1 (MQ=25) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGATCCTGAGTGTTATACCGCCCCTGCTGCGGtt < 1:2890548/71‑1 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtg < 1:3245108/49‑1 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:2921150/1‑52 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt > 1:532667/1‑70 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt > 1:1447469/1‑70 (MQ=255) tACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGtt > 1:1461899/1‑70 (MQ=255) aCTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGa < 1:1864114/38‑1 (MQ=12) cTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATAc > 1:2670562/1‑52 (MQ=255) cTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTaa < 1:364774/70‑1 (MQ=255) cTGATAAAAATCCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATAc > 1:2403125/1‑52 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTg < 1:328303/41‑1 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:2543605/48‑1 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGtt < 1:3353523/67‑1 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAgtgat > 1:3220834/1‑44 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATAc > 1:2645672/1‑50 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATAc > 1:346663/1‑50 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtgat < 1:3902469/46‑3 (MQ=255) gATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtata > 1:2062969/1‑49 (MQ=255) gATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGtt < 1:504292/66‑1 (MQ=255) aTAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtgat > 1:4064961/1‑43 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGATTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:2329000/71‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:2963637/46‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg < 1:156782/60‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg < 1:3080931/60‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:1950267/71‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:3621980/71‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGt < 1:3625784/70‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGt < 1:2755390/70‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCT‑AGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:3137623/70‑1 (MQ=255) tAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCct > 1:2141774/1‑55 (MQ=255) aaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg < 1:610657/57‑1 (MQ=255) aaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGa > 1:2694211/1‑71 (MQ=255) ttCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGata < 1:1348121/71‑1 (MQ=255) ttCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGata < 1:351529/71‑1 (MQ=255) ttCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGat > 1:3399694/1‑70 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataata < 1:1199011/71‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataata < 1:612605/71‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:691148/70‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:1454696/70‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:686262/70‑1 (MQ=255) cctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt < 1:723006/71‑1 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAAt < 1:1095040/55‑1 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt > 1:1540990/1‑70 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt > 1:1536064/1‑70 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGATAATCTTACCGATAATAGTta > 1:1392362/1‑71 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:2413933/1‑70 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:2032962/1‑70 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:1552513/69‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:1166089/69‑1 (MQ=255) ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTaa > 1:2355988/1‑50 (MQ=255) ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg < 1:2932678/71‑1 (MQ=255) ttCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAAt > 1:8035/1‑50 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg > 1:2983549/1‑40 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt > 1:1564481/1‑51 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGt < 1:650788/69‑1 (MQ=255) | AATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGT > minE/2572648‑2572781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |