| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,941 | G→A | 20.4% | intergenic (+1709/‑70) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,941 | 0 | G | A | 20.4% | 194.0 / 42.9 | 88 | intergenic (+1709/‑70) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (41/29); new base A (10/8); total (51/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
GATAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACGGTAATTT > minE/2572872‑2573009 | gATAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGgaga < 1:1281686/71‑1 (MQ=255) aTAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGAt > 1:1447287/1‑71 (MQ=255) tAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGAtt > 1:492922/1‑71 (MQ=255) aaaTCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTc > 1:771026/1‑71 (MQ=255) aaTCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTca < 1:3636097/71‑2 (MQ=255) aTCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGt < 1:821986/71‑1 (MQ=255) tCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGt > 1:177536/1‑70 (MQ=255) aTTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGt > 1:1110705/1‑68 (MQ=255) tttGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCg > 1:3902057/1‑66 (MQ=255) tttGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGt > 1:246649/1‑67 (MQ=255) tttGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGt < 1:1264292/67‑1 (MQ=255) tttGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGa < 1:2472896/71‑1 (MQ=255) tggttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGat < 1:1002078/70‑1 (MQ=255) gttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGatat < 1:57418/70‑1 (MQ=255) ttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCa < 1:63747/71‑1 (MQ=255) ggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGTCTTAAGGAGATTCGTAAGATATCAGc > 1:1727338/1‑71 (MQ=255) ggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAg < 1:739253/70‑1 (MQ=255) cgcACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGGTATCAGCCa > 1:1727925/1‑71 (MQ=255) gcACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCAc < 1:1078111/71‑1 (MQ=255) tGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGata < 1:1371497/59‑1 (MQ=255) tGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGata < 1:3737198/59‑1 (MQ=255) tGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCa < 1:2788121/66‑1 (MQ=255) ggAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACta > 1:3208371/1‑68 (MQ=255) gAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACt > 1:737501/1‑66 (MQ=255) gAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATAc < 1:971236/70‑1 (MQ=255) taatCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGat < 1:1326741/71‑1 (MQ=255) taatCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGat < 1:2784413/71‑1 (MQ=255) taatCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGat < 1:3307480/71‑1 (MQ=255) aatCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCa > 1:920237/1‑60 (MQ=255) aatCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGat > 1:3126183/1‑70 (MQ=255) atCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCa > 1:4004731/1‑59 (MQ=255) atCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACta > 1:1679580/1‑62 (MQ=255) atCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGata > 1:2179051/1‑70 (MQ=255) atCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGata > 1:1247628/1‑70 (MQ=255) atCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGata > 1:3046975/1‑70 (MQ=255) tGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGata > 1:808254/1‑49 (MQ=255) tGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAg > 1:1833989/1‑53 (MQ=255) tGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGa < 1:1513734/47‑1 (MQ=255) aGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGAtata < 1:569023/67‑1 (MQ=255) ttttATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTaa < 1:4042406/41‑1 (MQ=255) ttttATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATataaataa > 1:2049871/1‑70 (MQ=255) ttttATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATataaataa > 1:448166/1‑70 (MQ=255) ttttATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAaataat < 1:2975705/71‑1 (MQ=255) ttttATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAaataat < 1:2676961/71‑1 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAaataat > 1:1643217/1‑69 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAaataat > 1:1389589/1‑69 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAaataat > 1:2470154/1‑69 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAaataat > 1:1803001/1‑69 (MQ=255) tATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAaataat > 1:2386328/1‑68 (MQ=255) aTCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGa > 1:1344677/1‑71 (MQ=255) cACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACt > 1:51271/1‑71 (MQ=255) aCTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGata > 1:696353/1‑38 (MQ=255) aCTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTc < 1:1428669/71‑1 (MQ=255) aCTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTc < 1:740301/71‑1 (MQ=255) cTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCAc < 1:3033308/45‑1 (MQ=255) cTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCAc < 1:1062372/45‑1 (MQ=255) cTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCa < 1:3306696/71‑1 (MQ=255) tACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGcc < 1:3222670/42‑1 (MQ=255) aCAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACt > 1:2973097/1‑71 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCa > 1:451991/1‑41 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCa > 1:3635932/1‑41 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACtat > 1:2177977/1‑45 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGata > 1:1386190/1‑52 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGata > 1:1619485/1‑52 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAc < 1:2831702/69‑1 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAc < 1:1242675/69‑1 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACtt > 1:2552011/1‑71 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACtt > 1:538782/1‑71 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACtt > 1:918127/1‑71 (MQ=255) cAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAcct > 1:476675/1‑69 (MQ=255) cAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAcct > 1:1077330/1‑69 (MQ=255) aGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTg > 1:1587684/1‑71 (MQ=255) aGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACt > 1:1348933/1‑42 (MQ=255) cTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGc > 1:3968793/1‑70 (MQ=255) cTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGc > 1:2919862/1‑70 (MQ=255) cTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGc > 1:2924507/1‑70 (MQ=255) cTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAAtaataa < 1:2763269/59‑1 (MQ=255) tataGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCa > 1:2424803/1‑63 (MQ=255) ataGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCaa < 1:3243392/69‑1 (MQ=255) agagGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAAc > 1:3257545/1‑69 (MQ=255) agagGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGa > 1:1204386/1‑42 (MQ=255) agagGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAc > 1:3289423/1‑61 (MQ=255) gagGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCa < 1:4101527/70‑1 (MQ=255) gagGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCa < 1:649174/70‑1 (MQ=255) agGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGAtata < 1:2797572/44‑1 (MQ=255) gCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGa < 1:3395544/69‑1 (MQ=255) ttAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACgg > 1:1554100/1‑70 (MQ=255) aaGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACGGt < 1:2355925/69‑1 (MQ=255) aaGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACCTGTAAACCAGACGGt < 1:161199/69‑1 (MQ=255) gagaTTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACGGTAAt < 1:1037036/69‑1 (MQ=255) agaTTCGTAAGATATCAGCCACTATACCGATATAAAtaataa < 1:3456616/42‑1 (MQ=255) agaTTCGTAAGATATCAGCCACTATACCGATATAAAtaataa < 1:2463906/42‑1 (MQ=255) agaTTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACGGTAAttt > 1:996181/1‑70 (MQ=255) gaTTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCa > 1:4003622/1‑58 (MQ=255) | GATAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACGGTAATTT > minE/2572872‑2573009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |