| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,314 | A→G | 5.1% | V988A (GTT→GCT) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,314 | 0 | A | G | 5.1% | 288.9 / 5.5 | 104 | V988A (GTT→GCT) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (38/60); new base G (3/2); total (41/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
GGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGC > minE/2584244‑2584384 | ggCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATaa > 1:2223503/1‑71 (MQ=255) cAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAcc < 1:1120332/71‑1 (MQ=255) tCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAcc < 1:670661/65‑1 (MQ=255) cccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:1215541/1‑71 (MQ=255) ccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGgcag < 1:696123/67‑1 (MQ=255) tCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:3913393/1‑70 (MQ=255) cACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCgg > 1:3975791/1‑60 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGgcagca > 1:3598175/1‑64 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:3895554/1‑68 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:1760819/1‑68 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCgag < 1:3409624/71‑1 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCgag < 1:2300597/71‑1 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt < 1:1080967/70‑1 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:776450/71‑1 (MQ=255) ggTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa < 1:2386074/71‑1 (MQ=255) acctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAg > 1:2658065/1‑48 (MQ=255) cctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAgg < 1:1136916/70‑1 (MQ=255) cctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAgg < 1:2376324/70‑1 (MQ=255) ctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:1114923/65‑1 (MQ=255) taccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATaa > 1:1437177/1‑41 (MQ=255) accGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGc > 1:3288401/1‑68 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:3681596/71‑1 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:3885301/71‑1 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:1428751/71‑1 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:2490451/1‑69 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa < 1:3667066/69‑1 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa > 1:3271443/1‑62 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAg < 1:2171797/63‑1 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:1834526/70‑1 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga > 1:2652649/1‑69 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga > 1:2647922/1‑69 (MQ=255) cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag < 1:639438/71‑1 (MQ=255) cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:1297359/1‑71 (MQ=255) cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:4116409/1‑71 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg > 1:3922321/1‑71 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg > 1:492515/1‑71 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga > 1:2634427/1‑64 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg < 1:2368476/68‑1 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca > 1:3018587/1‑71 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtc < 1:2590390/70‑1 (MQ=255) ctGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtc > 1:3042924/2‑70 (MQ=255) tGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca < 1:3132587/70‑1 (MQ=255) tGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca < 1:3297886/70‑1 (MQ=255) tGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca < 1:3691148/70‑1 (MQ=255) tGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAAt < 1:839299/53‑1 (MQ=255) tGCGCGCCACTGCCGGCACCGTTACTGATACCTAGCGGTAATACGCCGAGAAt < 1:3768472/53‑1 (MQ=255) gcgcCAGAACCGGCACCATGACTTATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg < 1:73211/71‑1 (MQ=255) gcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg < 1:487228/71‑1 (MQ=255) gcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg < 1:3677551/71‑1 (MQ=255) cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:2766735/71‑1 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:3376530/70‑1 (MQ=255) cAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGGCATCAGGAtt < 1:1965840/71‑1 (MQ=255) gAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTgg < 1:828765/71‑1 (MQ=255) aCCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:2270056/43‑1 (MQ=255) aCCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGc > 1:1064371/1‑71 (MQ=255) ccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg < 1:1490653/71‑1 (MQ=9) ccGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:1672802/63‑1 (MQ=255) ccGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCg > 1:119297/1‑71 (MQ=255) cGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:3487752/62‑1 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt > 1:3151704/1‑70 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:1305174/71‑1 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:3973594/71‑1 (MQ=255) gCACCATGACTGATAACCAGCGTCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa < 1:1738442/71‑1 (MQ=255) aCCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa < 1:3193860/70‑1 (MQ=255) aCCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAAc < 1:2938334/71‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:138680/36‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:382435/36‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:2306178/36‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:3230313/46‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt < 1:1491381/66‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:325839/67‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg < 1:479912/71‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGt < 1:2112082/49‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGt < 1:4049633/49‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:1497753/71‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:460122/71‑1 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATaaa > 1:2730724/1‑36 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt > 1:2989982/1‑57 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTggg > 1:4004351/1‑61 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa > 1:2226244/1‑71 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa < 1:1152603/71‑1 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa > 1:3108791/1‑71 (MQ=255) tGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:3213435/69‑1 (MQ=255) taCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCAta > 1:4021534/2‑70 (MQ=2) aCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCAT‑AGGATAGGACGCAGACGCATAcg > 1:1001754/1‑71 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:3195361/1‑43 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa > 1:4127276/1‑53 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt > 1:155850/1‑54 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:27258/1‑64 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATcc > 1:102082/1‑71 (MQ=255) tGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt < 1:2689535/52‑1 (MQ=255) tGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcg < 1:3504817/70‑1 (MQ=255) gATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:1826979/37‑1 (MQ=255) gATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATT‑GGCGTAAACGCATCcgc < 1:2455645/69‑1 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAtt > 1:1133546/1‑51 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg > 1:1974473/1‑62 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcgc < 1:616185/69‑1 (MQ=255) tAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgcc > 1:2377341/1‑71 (MQ=255) aaCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa > 1:2717271/1‑62 (MQ=255) aCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc < 1:342431/71‑1 (MQ=255) | GGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGC > minE/2584244‑2584384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |