Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,584,324 G→A 5.6% L985L (CTG→TTG) ‡ mdtF ← multidrug transporter, RpoS‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,584,3240GA 5.6% 359.8 / 6.5 107L985L (CTG→TTG) ‡mdtFmultidrug transporter, RpoS‑dependent
Reads supporting (aligned to +/- strand):  ref base G (39/62);  new base A (3/3);  total (42/65)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATT  >  minE/2584257‑2584394
                                                                   |                                                                      
cccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc                                                                     >  1:1215541/1‑71 (MQ=255)
 ccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGgcag                                                                        <  1:696123/67‑1 (MQ=255)
    tCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc                                                                  >  1:3913393/1‑70 (MQ=255)
      aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGgcagca                                                                      >  1:3598175/1‑64 (MQ=255)
      aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc                                                                  >  1:4143680/1‑68 (MQ=255)
      aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc                                                                  >  1:1760819/1‑68 (MQ=255)
      aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCgag                                                               <  1:2300597/71‑1 (MQ=255)
        gCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGaa                                                             >  1:2773088/1‑71 (MQ=255)
          cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt                                                            <  1:1080967/70‑1 (MQ=255)
          cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa                                                           <  1:776450/71‑1 (MQ=255)
           ggTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa                                                          <  1:2386074/71‑1 (MQ=255)
                ctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa                                                           <  1:1114923/65‑1 (MQ=255)
                  accGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGc                                                      >  1:3288401/1‑68 (MQ=255)
                   ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga                                                  <  1:3885301/71‑1 (MQ=255)
                   ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa                                                    <  1:3667066/69‑1 (MQ=255)
                   ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa                                                    >  1:2490451/1‑69 (MQ=255)
                     gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag                                                 <  1:1834526/70‑1 (MQ=255)
                     gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga                                                  >  1:2652649/1‑69 (MQ=255)
                     gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga                                                  >  1:2647922/1‑69 (MQ=255)
                      cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag                                               >  1:1297359/1‑71 (MQ=255)
                      cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag                                               >  1:4116409/1‑71 (MQ=255)
                       gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg                                              >  1:492515/1‑71 (MQ=255)
                       gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg                                              >  1:3922321/1‑71 (MQ=255)
                          ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga                                                  >  1:2634427/1‑64 (MQ=255)
                          ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg                                              <  1:2368476/68‑1 (MQ=255)
                          ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca                                           >  1:3018587/1‑71 (MQ=255)
                          ctGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtc                                            >  1:3042924/2‑70 (MQ=255)
                           tGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca                                           <  1:3691148/70‑1 (MQ=255)
                           tGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca                                           <  1:3297886/70‑1 (MQ=255)
                           tGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAAt                                                            <  1:839299/53‑1 (MQ=255)
                           tGCGCGCCACTGCCGGCACCGTTACTGATACCTAGCGGTAATACGCCGAGAAt                                                            <  1:3768472/53‑1 (MQ=255)
                            gcgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat                                          <  1:3285327/70‑1 (MQ=255)
                              gcgcCAGAACCGGCACCATGACTTATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg                                       <  1:73211/71‑1 (MQ=255)
                              gcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg                                       <  1:487228/71‑1 (MQ=255)
                              gcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg                                       <  1:3677551/71‑1 (MQ=255)
                               cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg                                      <  1:2766735/71‑1 (MQ=255)
                                gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg                                      <  1:3376530/70‑1 (MQ=255)
                                  cAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGGCATCAGGAtt                                   <  1:1965840/71‑1 (MQ=255)
                                    gAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTgg                                 <  1:828765/71‑1 (MQ=255)
                                      aCCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa                                                           <  1:2270056/43‑1 (MQ=255)
                                      aCCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGc                               >  1:1064371/1‑71 (MQ=255)
                                       ccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg                              <  1:1490653/71‑1 (MQ=9)
                                       ccGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg                                      <  1:1672802/63‑1 (MQ=255)
                                       ccGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCg                              >  1:119297/1‑71 (MQ=255)
                                        cGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg                                      <  1:3487752/62‑1 (MQ=255)
                                         ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt                             >  1:3151704/1‑70 (MQ=255)
                                         ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                            <  1:3973594/71‑1 (MQ=255)
                                         ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                            <  1:1305174/71‑1 (MQ=255)
                                            aCCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                          <  1:3193860/70‑1 (MQ=255)
                                            aCCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAAc                         <  1:2938334/71‑1 (MQ=255)
                                             ccATGACTGATAACCAGCGGCAGCACGCCGAGAATa                                                           <  1:2306178/36‑1 (MQ=255)
                                             ccATGACTGATAACCAGCGGCAGCACGCCGAGAATa                                                           <  1:138680/36‑1 (MQ=255)
                                             ccATGACTGATAACCAGCGGCAGCACGCCGAGAATa                                                           <  1:382435/36‑1 (MQ=255)
                                             ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag                                                 <  1:3230313/46‑1 (MQ=255)
                                             ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt                             <  1:1491381/66‑1 (MQ=255)
                                             ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                            <  1:325839/67‑1 (MQ=255)
                                             ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg                        <  1:479912/71‑1 (MQ=255)
                                              cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGt                                             <  1:2112082/49‑1 (MQ=255)
                                              cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGt                                             <  1:4049633/49‑1 (MQ=255)
                                              cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                       <  1:1497753/71‑1 (MQ=255)
                                              cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                       <  1:460122/71‑1 (MQ=255)
                                               aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt                                    >  1:2989982/1‑57 (MQ=255)
                                               aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt                                    >  1:1367422/1‑57 (MQ=255)
                                               aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTggg                                >  1:4004351/1‑61 (MQ=255)
                                               aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                       <  1:3738684/70‑1 (MQ=255)
                                               aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa                      <  1:1152603/71‑1 (MQ=255)
                                               aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa                      >  1:3108791/1‑71 (MQ=255)
                                               aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa                      >  1:2226244/1‑71 (MQ=255)
                                                tGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca                                           <  1:1936658/49‑1 (MQ=255)
                                                tGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                       <  1:3213435/69‑1 (MQ=255)
                                                 taCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCAta                    >  1:4021534/2‑70 (MQ=2)
                                                  aCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATAGGATAGGACGCAGACGCATAcg                  >  1:1001754/1‑71 (MQ=255)
                                                  aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag                                               >  1:3195361/1‑43 (MQ=255)
                                                  aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt                                    >  1:155850/1‑54 (MQ=255)
                                                  aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                          >  1:27258/1‑64 (MQ=255)
                                                  aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                          >  1:2387376/1‑64 (MQ=255)
                                                  aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATcc                   >  1:102082/1‑71 (MQ=255)
                                                    tGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt                                    <  1:2689535/52‑1 (MQ=255)
                                                    tGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcg                  <  1:3504817/70‑1 (MQ=255)
                                                     gATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga                                                  <  1:1826979/37‑1 (MQ=255)
                                                     gATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGCGTAAACGCATCcgc                 <  1:2455645/69‑1 (MQ=255)
                                                      aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAtt                                   >  1:1133546/1‑51 (MQ=255)
                                                      aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg                        >  1:1974473/1‑62 (MQ=255)
                                                      aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcgc                 <  1:616185/69‑1 (MQ=255)
                                                       tAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgcc              >  1:2377341/1‑71 (MQ=255)
                                                        aaCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa                      >  1:2717271/1‑62 (MQ=255)
                                                         aCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc            <  1:342431/71‑1 (MQ=255)
                                                            aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt          >  1:3384175/1‑70 (MQ=255)
                                                            aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc         <  1:2644862/71‑1 (MQ=255)
                                                            aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc         <  1:656111/71‑1 (MQ=255)
                                                            aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc         <  1:3313112/71‑1 (MQ=255)
                                                             gaggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCg        <  1:487447/69‑1 (MQ=255)
                                                             gCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                          >  1:2294722/1‑53 (MQ=255)
                                                              cGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                       >  1:3607859/1‑55 (MQ=255)
                                                                 cagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatga    <  1:1594923/71‑1 (MQ=255)
                                                                  agcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt                     >  1:3310816/1‑53 (MQ=255)
                                                                   gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                            <  1:2500439/45‑1 (MQ=255)
                                                                   gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa                      <  1:111352/51‑1 (MQ=255)
                                                                   gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGAtt  <  1:2357021/71‑1 (MQ=255)
                                                                   gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGAtt  >  1:3735974/1‑71 (MQ=255)
                                                                   |                                                                      
CCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATT  >  minE/2584257‑2584394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: