| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,343 | C→G | 9.5% | L978L (CTG→CTC) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,343 | 0 | C | G | 9.5% | 352.9 / 20.1 | 116 | L978L (CTG→CTC) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (42/63); new base G (3/8); total (45/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
CCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCC > minE/2584276‑2584413 | ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:3681596/71‑1 (MQ=255)ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:3885301/71‑1 (MQ=255)ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:1428751/71‑1 (MQ=255)ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:2490451/1‑69 (MQ=255)ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa < 1:3667066/69‑1 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:1834526/70‑1 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga > 1:2647922/1‑69 (MQ=255) cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:1297359/1‑71 (MQ=255) cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:4116409/1‑71 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg > 1:492515/1‑71 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg > 1:3922321/1‑71 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga > 1:2634427/1‑64 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca > 1:3018587/1‑71 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtc < 1:2590390/70‑1 (MQ=255) ctGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtc > 1:3042924/2‑70 (MQ=255) tGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca < 1:3297886/70‑1 (MQ=255) tGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca < 1:3691148/70‑1 (MQ=255) gcgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat < 1:3285327/70‑1 (MQ=255) gcgcCAGAACCGGCACCATGACTTATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg < 1:73211/71‑1 (MQ=255) gcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg < 1:487228/71‑1 (MQ=255) gcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg < 1:3677551/71‑1 (MQ=255) cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:2766735/71‑1 (MQ=255) cAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt < 1:902317/70‑1 (MQ=255) gAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTgg < 1:828765/71‑1 (MQ=255) aCCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGc > 1:1064371/1‑71 (MQ=255) ccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg < 1:1490653/71‑1 (MQ=9) ccGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCg > 1:119297/1‑71 (MQ=255) cGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:3487752/62‑1 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt > 1:3151704/1‑70 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:1305174/71‑1 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:3973594/71‑1 (MQ=255) gCACCATGACTGATAACCAGCGTCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa < 1:1738442/71‑1 (MQ=255) aCCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa < 1:3193860/70‑1 (MQ=255) aCCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAAc < 1:2938334/71‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt < 1:1491381/66‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:325839/67‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGt < 1:2112082/49‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGt < 1:4049633/49‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:1497753/71‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:460122/71‑1 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt > 1:1367422/1‑57 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt > 1:2989982/1‑57 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTggg > 1:4004351/1‑61 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa > 1:2226244/1‑71 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa > 1:3108791/1‑71 (MQ=255) tGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca < 1:1936658/49‑1 (MQ=255) tGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:3213435/69‑1 (MQ=255) aCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCAT‑AGGATAGGACGCAGACGCATAcg > 1:1001754/1‑71 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:3195361/1‑43 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa > 1:4127276/1‑53 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt > 1:155850/1‑54 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:2387376/1‑64 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:27258/1‑64 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATcc > 1:102082/1‑71 (MQ=255) tGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt < 1:2689535/52‑1 (MQ=255) tGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcg < 1:3504817/70‑1 (MQ=255) gATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:1826979/37‑1 (MQ=255) gATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATT‑GGCGTAAACGCATCcgc < 1:2455645/69‑1 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAtt > 1:1133546/1‑51 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg > 1:1974473/1‑62 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcgc < 1:616185/69‑1 (MQ=255) tAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgcc > 1:2377341/1‑71 (MQ=255) aaCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa > 1:2717271/1‑62 (MQ=255) aCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc < 1:342431/71‑1 (MQ=255) aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt > 1:3384175/1‑70 (MQ=255) aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc < 1:3313112/71‑1 (MQ=255) aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc < 1:656111/71‑1 (MQ=255) aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc < 1:2644862/71‑1 (MQ=255) gaggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCg < 1:487447/69‑1 (MQ=255) gCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:2294722/1‑53 (MQ=255) cagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatga < 1:1594923/71‑1 (MQ=255) agcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt > 1:3310816/1‑53 (MQ=255) gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:2500439/45‑1 (MQ=255) gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa < 1:111352/51‑1 (MQ=255) gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGAtt < 1:2357021/71‑1 (MQ=255) gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGAtt > 1:3735974/1‑71 (MQ=255) caCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt < 1:2186735/43‑1 (MQ=255) aCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc > 1:15852/1‑71 (MQ=255) aCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc < 1:794632/71‑1 (MQ=255) aCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc > 1:899600/1‑71 (MQ=255) gCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCct > 1:1602426/1‑71 (MQ=255) cGAGAATAAAGGCCAGAGAGGTCATCAGGATTggg < 1:2754577/35‑1 (MQ=255) cGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCctct < 1:2207389/71‑1 (MQ=255) cGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCctct < 1:821019/71‑1 (MQ=255) gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:601585/1‑40 (MQ=255) agaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:2082660/42‑1 (MQ=255) agaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:3571546/42‑1 (MQ=255) gaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATc > 1:1749552/1‑71 (MQ=255) aaTAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcgt < 1:1654566/46‑2 (MQ=9) aaTAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcgt < 1:2101599/46‑2 (MQ=9) aaTAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcgt < 1:2553341/46‑2 (MQ=9) aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc > 1:1976319/1‑51 (MQ=255) aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc > 1:3843247/1‑51 (MQ=255) aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc > 1:3178103/1‑51 (MQ=255) tAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACg < 1:788433/37‑1 (MQ=9) tAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACg < 1:693222/37‑1 (MQ=9) aaaGGCGAGAGAGGTCATCAGGATAGGACGCAGACg < 1:118483/36‑1 (MQ=9) ccAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatga > 1:4035219/1‑51 (MQ=255) ccAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat < 1:915931/49‑1 (MQ=255) cAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc > 1:1346506/1‑71 (MQ=255) | CCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCC > minE/2584276‑2584413 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |