Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,584,364 G→A 9.9% R971R (CGC→CGT mdtF ← multidrug transporter, RpoS‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,584,3640GA 9.9% 324.5 / 18.4 101R971R (CGC→CGTmdtFmultidrug transporter, RpoS‑dependent
Reads supporting (aligned to +/- strand):  ref base G (43/48);  new base A (2/8);  total (45/56)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 105 total aligned reads displayed.

ACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATCATCTCA  >  minE/2584295‑2584433
                                                                     |                                                                     
aCCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGc                                                                      >  1:1064371/1‑71 (MQ=255)
 ccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg                                                                     <  1:1490653/71‑1 (MQ=9)
 ccGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCg                                                                     >  1:119297/1‑71 (MQ=255)
   ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt                                                                    >  1:3151704/1‑70 (MQ=255)
   ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                                                   <  1:3973594/71‑1 (MQ=255)
   ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                                                   <  1:1305174/71‑1 (MQ=255)
    gCACCATGACTGATAACCAGCGTCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa                                                                  <  1:1738442/71‑1 (MQ=255)
      aCCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAAc                                                                <  1:2938334/71‑1 (MQ=255)
       ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt                                                                    <  1:1491381/66‑1 (MQ=255)
       ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                                                   <  1:325839/67‑1 (MQ=255)
       ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg                                                               <  1:479912/71‑1 (MQ=255)
        cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                                                              <  1:1497753/71‑1 (MQ=255)
        cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                                                              <  1:460122/71‑1 (MQ=255)
         aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTggg                                                                       >  1:4004351/1‑61 (MQ=255)
         aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                                                              <  1:3738684/70‑1 (MQ=255)
         aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa                                                             >  1:3108791/1‑71 (MQ=255)
         aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa                                                             >  1:2226244/1‑71 (MQ=255)
         aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa                                                             <  1:1152603/71‑1 (MQ=255)
          tGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                                                              <  1:3213435/69‑1 (MQ=255)
            aCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATAGGATAGGACGCAGACGCATAcg                                                         >  1:1001754/1‑71 (MQ=255)
            aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                                                                 >  1:2387376/1‑64 (MQ=255)
            aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                                                                 >  1:27258/1‑64 (MQ=255)
            aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATcc                                                          >  1:102082/1‑71 (MQ=255)
              tGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcg                                                         <  1:3504817/70‑1 (MQ=255)
               gATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGCGTAAACGCATCcgc                                                        <  1:2455645/69‑1 (MQ=255)
                aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg                                                               >  1:1974473/1‑62 (MQ=255)
                aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcgc                                                        <  1:616185/69‑1 (MQ=255)
                  aaCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa                                                             >  1:2717271/1‑62 (MQ=255)
                   aCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc                                                   <  1:342431/71‑1 (MQ=255)
                      aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt                                                 >  1:3384175/1‑70 (MQ=255)
                      aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc                                                <  1:3313112/71‑1 (MQ=255)
                      aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc                                                <  1:2644862/71‑1 (MQ=255)
                      aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc                                                <  1:656111/71‑1 (MQ=255)
                       gaggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCg                                               <  1:487447/69‑1 (MQ=255)
                       gCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                                                                 >  1:2294722/1‑53 (MQ=255)
                        cGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                                                              >  1:3607859/1‑55 (MQ=255)
                           cagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatga                                           <  1:1594923/71‑1 (MQ=255)
                            agcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt                                                            >  1:3310816/1‑53 (MQ=255)
                             gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                                                   <  1:2500439/45‑1 (MQ=255)
                             gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa                                                             <  1:111352/51‑1 (MQ=255)
                             gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGAtt                                         >  1:3735974/1‑71 (MQ=255)
                             gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGAtt                                         <  1:2357021/71‑1 (MQ=255)
                              caCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt                                                                    <  1:1527934/43‑1 (MQ=255)
                              caCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt                                                                    <  1:2186735/43‑1 (MQ=255)
                               aCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc                                       <  1:794632/71‑1 (MQ=255)
                               aCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc                                       >  1:899600/1‑71 (MQ=255)
                               aCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc                                       >  1:15852/1‑71 (MQ=255)
                                   cGAGAATAAAGGCCAGAGAGGTCATCAGGATTggg                                                                       <  1:2754577/35‑1 (MQ=255)
                                   cGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCctct                                   <  1:821019/71‑1 (MQ=255)
                                   cGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCctct                                   <  1:2207389/71‑1 (MQ=255)
                                    gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                                                                 >  1:601585/1‑40 (MQ=255)
                                    gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                                                                 >  1:2787207/1‑40 (MQ=255)
                                    gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                                                                 >  1:818340/1‑40 (MQ=255)
                                    gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                                                   <  1:1360215/38‑1 (MQ=255)
                                     agaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                                                              <  1:3571546/42‑1 (MQ=255)
                                     agaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                                                              <  1:2082660/42‑1 (MQ=255)
                                      gaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATc                                >  1:1749552/1‑71 (MQ=255)
                                       aaTAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcgt                                                        <  1:2553341/46‑2 (MQ=9)
                                       aaTAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcgt                                                        <  1:1654566/46‑2 (MQ=9)
                                       aaTAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcgt                                                        <  1:2101599/46‑2 (MQ=9)
                                       aaTAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcgt                                                        <  1:252030/46‑2 (MQ=9)
                                       aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc                                                   >  1:3178103/1‑51 (MQ=255)
                                       aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc                                                   >  1:1976319/1‑51 (MQ=255)
                                       aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc                                                   >  1:3843247/1‑51 (MQ=255)
                                         tAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACg                                                               <  1:693222/37‑1 (MQ=9)
                                         tAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACg                                                               <  1:788433/37‑1 (MQ=9)
                                          aaaGGCGAGAGAGGTCATCAGGATAGGACGCAGACg                                                               <  1:118483/36‑1 (MQ=9)
                                               ccAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatga                                           >  1:4035219/1‑51 (MQ=255)
                                               ccAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat                                             <  1:915931/49‑1 (MQ=255)
                                               ccAGAGAGGTCATCAGGATTGGGCGTAAACGCATCAGCGCCGCTTCgatga                                           >  1:2185675/1‑51 (MQ=255)
                                                cAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc                      >  1:1346506/1‑71 (MQ=255)
                                                     agGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatga                                           <  1:3615588/45‑1 (MQ=255)
                                                        tcatcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatga                                           <  1:2204305/42‑1 (MQ=255)
                                                         cgtcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc                                       <  1:1547348/43‑1 (MQ=255)
                                                         catcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc                      >  1:965729/1‑62 (MQ=255)
                                                         catcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc                      >  1:1755320/1‑62 (MQ=255)
                                                         catcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCttcttt               >  1:1995259/1‑69 (MQ=255)
                                                          atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTc                                                <  1:1341294/35‑1 (MQ=255)
                                                          atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGAtt                                         <  1:811881/42‑1 (MQ=255)
                                                          atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc                      <  1:2948180/61‑1 (MQ=255)
                                                           tcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCga                                              <  1:4039603/36‑1 (MQ=255)
                                                           tcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCg                                               <  1:923050/35‑1 (MQ=255)
                                                           tcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc                                       <  1:295196/43‑1 (MQ=255)
                                                           tcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATc                                <  1:1580106/50‑1 (MQ=255)
                                                            caGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatgat                                          >  1:3615218/1‑39 (MQ=255)
                                                             aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCg                               >  1:2782770/1‑49 (MQ=255)
                                                             aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCTGCGTTTTCCcttc                  >  1:2304155/1‑62 (MQ=255)
                                                             aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTccc                     >  1:2646466/1‑59 (MQ=255)
                                                             aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTccc                     >  1:2180471/1‑59 (MQ=255)
                                                             aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCttctttct             >  1:733781/1‑67 (MQ=255)
                                                             aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcat         <  1:1967454/71‑1 (MQ=255)
                                                             aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcat         <  1:1719309/71‑1 (MQ=255)
                                                             aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGc           >  1:491991/1‑69 (MQ=255)
                                                             aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGc           >  1:3687074/1‑69 (MQ=255)
                                                              ggATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcatc        <  1:3633268/71‑1 (MQ=255)
                                                              ggATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGca          >  1:2501980/1‑69 (MQ=255)
                                                               gATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCcttct                 >  1:3139256/1‑61 (MQ=255)
                                                               gATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCcttct                 >  1:1474700/1‑61 (MQ=255)
                                                                    ggCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCttctttct             >  1:3719333/1‑60 (MQ=255)
                                                                    ggCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATCATCTCa  <  1:383683/71‑1 (MQ=255)
                                                                     |                                                                     
ACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATCATCTCA  >  minE/2584295‑2584433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: