| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,369 | A→G | 10.7% | L970L (TTA→CTA) ‡ | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,369 | 0 | A | G | 10.7% | 229.8 / 17.4 | 84 | L970L (TTA→CTA) ‡ | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (35/40); new base G (2/7); total (37/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
GCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCA > minE/2584299‑2584439 | gCACCATGACTGATAACCAGCGTCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa < 1:1738442/71‑1 (MQ=255) aCCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa < 1:3193860/70‑1 (MQ=255) aCCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAAc < 1:2938334/71‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg < 1:479912/71‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:460122/71‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:1497753/71‑1 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:3738684/70‑1 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa < 1:1152603/71‑1 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa > 1:3108791/1‑71 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa > 1:2226244/1‑71 (MQ=255) tGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:3213435/69‑1 (MQ=255) taCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCAta > 1:4021534/2‑70 (MQ=2) aCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCAT‑AGGATAGGACGCAGACGCATAcg > 1:1001754/1‑71 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:27258/1‑64 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:2387376/1‑64 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATcc > 1:102082/1‑71 (MQ=255) tGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcg < 1:3504817/70‑1 (MQ=255) gATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATT‑GGCGTAAACGCATCcgc < 1:2455645/69‑1 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg > 1:1974473/1‑62 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcgc < 1:616185/69‑1 (MQ=255) tAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgcc > 1:2377341/1‑71 (MQ=255) aaCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa > 1:2717271/1‑62 (MQ=255) aCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc < 1:342431/71‑1 (MQ=255) aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt > 1:3384175/1‑70 (MQ=255) aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc < 1:2644862/71‑1 (MQ=255) aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc < 1:3313112/71‑1 (MQ=255) aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc < 1:656111/71‑1 (MQ=255) gaggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCg < 1:487447/69‑1 (MQ=255) gCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:2294722/1‑53 (MQ=255) cGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc > 1:3607859/1‑55 (MQ=255) cagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatga < 1:1594923/71‑1 (MQ=255) agcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt > 1:3310816/1‑53 (MQ=255) gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa < 1:111352/51‑1 (MQ=255) gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGAtt > 1:3735974/1‑71 (MQ=255) gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGAtt < 1:2357021/71‑1 (MQ=255) aCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc > 1:15852/1‑71 (MQ=255) aCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc > 1:899600/1‑71 (MQ=255) aCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc < 1:794632/71‑1 (MQ=255) gCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCct > 1:1602426/1‑71 (MQ=255) cGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCctct < 1:821019/71‑1 (MQ=255) cGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCctct < 1:2207389/71‑1 (MQ=255) gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:601585/1‑40 (MQ=255) gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:818340/1‑40 (MQ=255) gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:2787207/1‑40 (MQ=255) agaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:3571546/42‑1 (MQ=255) agaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:2082660/42‑1 (MQ=255) gaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATc > 1:1749552/1‑71 (MQ=255) aaTAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcgt < 1:2553341/46‑2 (MQ=9) aaTAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcgt < 1:252030/46‑2 (MQ=9) aaTAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcgt < 1:2101599/46‑2 (MQ=9) aaTAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcgt < 1:1654566/46‑2 (MQ=9) aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc > 1:3178103/1‑51 (MQ=255) aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc > 1:1976319/1‑51 (MQ=255) aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc > 1:3843247/1‑51 (MQ=255) tAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACg < 1:788433/37‑1 (MQ=9) tAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACg < 1:693222/37‑1 (MQ=9) aaaGGCGAGAGAGGTCATCAGGATAGGACGCAGACg < 1:118483/36‑1 (MQ=9) ccAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatga > 1:4035219/1‑51 (MQ=255) ccAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat < 1:915931/49‑1 (MQ=255) ccAGAGAGGTCATCAGGATTGGGCGTAAACGCATCAGCGCCGCTTCgatga > 1:2185675/1‑51 (MQ=255) cAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc > 1:1346506/1‑71 (MQ=255) agGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatga < 1:3615588/45‑1 (MQ=255) tcatcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatga < 1:2204305/42‑1 (MQ=255) cgtcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc < 1:1547348/43‑1 (MQ=255) catcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc > 1:1755320/1‑62 (MQ=255) catcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc > 1:965729/1‑62 (MQ=255) catcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCttcttt > 1:1995259/1‑69 (MQ=255) atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTc < 1:1341294/35‑1 (MQ=255) atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGAtt < 1:811881/42‑1 (MQ=255) atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc < 1:2948180/61‑1 (MQ=255) tcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCga < 1:4039603/36‑1 (MQ=255) tcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCg < 1:923050/35‑1 (MQ=255) tcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGc < 1:295196/43‑1 (MQ=255) tcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATc < 1:1580106/50‑1 (MQ=255) caGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatgat > 1:3615218/1‑39 (MQ=255) aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCg > 1:2782770/1‑49 (MQ=255) aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCTGCGTTTTCCcttc > 1:2304155/1‑62 (MQ=255) aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTccc > 1:2646466/1‑59 (MQ=255) aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTccc > 1:2180471/1‑59 (MQ=255) aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCttctttct > 1:733781/1‑67 (MQ=255) aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcat < 1:1719309/71‑1 (MQ=255) aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcat < 1:1967454/71‑1 (MQ=255) aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGc > 1:3687074/1‑69 (MQ=255) aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGc > 1:491991/1‑69 (MQ=255) ggATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcatc < 1:3633268/71‑1 (MQ=255) ggATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGca > 1:2501980/1‑69 (MQ=255) gATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCcttct > 1:1474700/1‑61 (MQ=255) gATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCcttct > 1:3139256/1‑61 (MQ=255) ggCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCttctttct > 1:3719333/1‑60 (MQ=255) ggCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATCATCTCa < 1:383683/71‑1 (MQ=255) tAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCct < 1:59303/49‑1 (MQ=255) tAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcatc < 1:3743386/61‑1 (MQ=255) aaCGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCttctttc > 1:2577034/1‑53 (MQ=255) aaCGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTg > 1:2772580/1‑55 (MQ=255) aaCGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcatca > 1:2219211/1‑60 (MQ=255) aaCGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcatca > 1:1136793/1‑60 (MQ=255) aaCGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATCATCTCAACGGc > 1:2415120/1‑70 (MQ=255) aaCGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCa < 1:2893217/71‑1 (MQ=255) | GCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCA > minE/2584299‑2584439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |