| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,588 | A→C | 7.2% | F897V (TTC→GTC) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,588 | 0 | A | C | 7.2% | 262.0 / 16.0 | 97 | F897V (TTC→GTC) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (45/45); new base C (5/2); total (50/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01 | |||||||||||
AAGCCGCGCAGATCGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGAAATCGCA > minE/2584521‑2584652 | aaGCCGCGCAGATCGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACg > 1:3816358/1‑70 (MQ=255) gcgcAGATCGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTg < 1:3114887/71‑1 (MQ=255) gcgcAGATCGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTg < 1:2309241/71‑1 (MQ=255) cgcAGATCGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGa < 1:3031139/71‑1 (MQ=255) gcAGATCGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGAc > 1:49976/1‑71 (MQ=255) gcAGATCGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGAc < 1:3844953/71‑1 (MQ=255) gcAGATCGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGAc > 1:746796/1‑71 (MQ=255) gATCGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGa < 1:2522270/67‑1 (MQ=255) aTCGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGc < 1:2987539/71‑1 (MQ=255) cGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGctc > 1:3018857/1‑71 (MQ=255) cGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGctc > 1:1970207/1‑71 (MQ=255) ggTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGaa > 1:1437207/1‑59 (MQ=255) ggTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGctct < 1:1498835/71‑1 (MQ=255) ggTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGctct < 1:1278301/71‑1 (MQ=255) ggTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGctc < 1:3950942/70‑1 (MQ=255) ggTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGctc < 1:2496733/70‑1 (MQ=255) gTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGctct < 1:2787540/70‑1 (MQ=255) gTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCtctc < 1:2232431/71‑1 (MQ=255) tGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCa < 1:2627101/71‑1 (MQ=255) tGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCa < 1:4005309/71‑1 (MQ=255) ccAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATag < 1:1324483/71‑1 (MQ=255) ccAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATag < 1:4033511/71‑1 (MQ=255) cAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATa < 1:1739454/69‑1 (MQ=255) aGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGATTTGACCAGCTCTCATa < 1:1353589/68‑1 (MQ=255) tAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCa > 1:2635989/1‑64 (MQ=255) tAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCa > 1:3209795/1‑64 (MQ=255) tAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGt > 1:3538628/1‑70 (MQ=255) aaTGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGaa > 1:1815115/1‑49 (MQ=255) aaTGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTgc > 1:1608125/1‑71 (MQ=255) aaTGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTg > 1:3692766/1‑70 (MQ=255) aTGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATag > 1:2891432/1‑65 (MQ=255) aTGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTgcg < 1:1881534/71‑1 (MQ=255) aTGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTgcg > 1:3498591/1‑71 (MQ=255) aTGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTgcg < 1:677872/71‑1 (MQ=255) tGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCAt < 1:3256360/62‑1 (MQ=255) gcgcCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATaga > 1:141953/1‑64 (MQ=255) cgcCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTgcggc < 1:3984823/70‑1 (MQ=255) ccAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGAc < 1:2646792/48‑1 (MQ=255) ccAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGct > 1:2485047/1‑53 (MQ=255) cAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCggcgag < 1:839371/70‑1 (MQ=255) cAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCggcgag < 1:807771/70‑1 (MQ=255) aaCGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCggcgaggg > 1:2760393/1‑71 (MQ=255) acgacgCCTAACGGAACAACCAACATCACCGAGAACgg < 1:408824/38‑1 (MQ=255) acgacgCCTAACGGAACAACCAACATCACCGAGAACgg < 1:2787243/38‑1 (MQ=255) cgacgCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCa < 1:217846/53‑1 (MQ=255) gacgCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATaga > 1:2307951/1‑56 (MQ=255) acgCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCggcgagggcgag > 1:3807854/1‑71 (MQ=255) gCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGgcgagggcgagg < 1:2633125/70‑1 (MQ=255) ccTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGaa < 1:1289382/71‑1 (MQ=255) cTAACGGCACTACCAACATAACCGAGACAGGAATTGACCAGCTTTCATag < 1:2395755/50‑1 (MQ=16) cTAACGGAACAACCAACATCTCCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGaa > 1:59456/1‑70 (MQ=255) cTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGctct < 1:1328816/45‑1 (MQ=255) tAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCa < 1:1286912/46‑1 (MQ=255) tAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGgcgagggcgagg < 1:3060834/67‑1 (MQ=255) tAACGGAACAACCAACATCACCGAGAACGGAATTGA‑CAGCTCTCATagag > 1:3056635/1‑50 (MQ=38) tAACGGAACAACCAACATCACCGACAACGGAATTGACCAGCTCTCATa < 1:2978922/48‑1 (MQ=255) aaCGGAACAACCAACATCACCGAGGACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAacac > 1:3289650/1‑71 (MQ=255) aaCGGAACAACCAACATCACCGAGAACGGAATTGACCAGctct < 1:1780761/43‑1 (MQ=255) aCGGAACAACCAACATCACCGAGAACGGAATTGAc > 1:3378448/1‑35 (MQ=255) aCGGAACAACCAACATCACCGAGAACGGAATTGAc > 1:3373599/1‑35 (MQ=255) aCGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATagag > 1:688725/1‑49 (MQ=255) aCGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTgcgg > 1:902489/1‑54 (MQ=255) aCGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTg > 1:961289/1‑51 (MQ=255) aCGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTg > 1:3219363/1‑51 (MQ=255) aCGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCggcgagggcga > 1:331539/1‑63 (MQ=255) aCGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCggcgagggcga > 1:1739379/1‑63 (MQ=255) aCGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAacac > 1:1266403/1‑70 (MQ=255) aCGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAacac > 1:273260/1‑70 (MQ=255) aCGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAacac > 1:3177347/1‑70 (MQ=255) gAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGt < 1:481258/47‑1 (MQ=255) acaacCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAacac < 1:1918614/65‑1 (MQ=255) ctaccaacATAACCGAGACAGGAATTGACCAGCTTTCATa > 1:3534615/3‑40 (MQ=16) ctaccaacATAACCGAGACAGGAATTGACCAGCTTTCATa > 1:3097720/3‑40 (MQ=16) ctaccaacATAACCGAGACAGGAATTGACCAGCTTTCATa > 1:3486312/3‑40 (MQ=16) taccaacATAACCGAGACAGGAATTGACCAGCTTTCATa < 1:4083989/38‑1 (MQ=18) taccaacATAACCGAGACAGGAATTGACCAGCTTTCATAGAGTGCagcaag > 1:222464/2‑51 (MQ=19) aaccaacATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGgcgagggcgagg < 1:3710034/58‑1 (MQ=255) aaccaacATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGa < 1:596119/59‑1 (MQ=255) ccaacATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGt > 1:2438232/1‑71 (MQ=255) caacATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGaa < 1:2762973/57‑1 (MQ=255) aacATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGa < 1:2710466/71‑1 (MQ=255) aacATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGa < 1:3306459/71‑1 (MQ=255) aacATAACCGAGACAGGAATTGACCAGCTTTCATaga > 1:1599954/1‑37 (MQ=21) acATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGaa < 1:1374061/71‑1 (MQ=255) acATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGa < 1:1214633/70‑1 (MQ=255) acATCACCGAGAACGGAATTGACCACCTCTCATaga > 1:314943/1‑36 (MQ=255) cATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGaaa > 1:3094205/1‑71 (MQ=255) tCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGAAAt > 1:2990247/1‑70 (MQ=255) aCCGAGAACGGAATTGACCAGCTCTCATAGAGTgc > 1:12477/1‑35 (MQ=255) aCCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGa > 1:3416754/1‑58 (MQ=255) aCCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGAAATc > 1:2364050/1‑69 (MQ=255) ccGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGAAATCGCa > 1:2438345/1‑71 (MQ=255) ccGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGAAATCGCa > 1:69727/1‑71 (MQ=255) ccGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGAAATCGCa > 1:350571/1‑71 (MQ=255) ccGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGAAATCGCa > 1:3219231/1‑71 (MQ=255) cGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGa < 1:39606/63‑1 (MQ=255) gaACGTAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAAcacca > 1:1008266/1‑50 (MQ=255) aCGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAAcacc < 1:905322/47‑1 (MQ=255) | AAGCCGCGCAGATCGGTGGCCAGTAATGCGCCAACGACGCCTAACGGAACAACCAACATCACCGAGAACGGAATTGACCAGCTCTCATAGAGTGCGGCGAGGGCGAGGAACACCACGACCAGTGAAATCGCA > minE/2584521‑2584652 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |