| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,820 | G→A | 8.7% | G819G (GGC→GGT) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,820 | 0 | G | A | 8.7% | 213.2 / 8.2 | 69 | G819G (GGC→GGT) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (34/29); new base A (2/4); total (36/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01 | |||||||||||
AAATTTCATGGCGTCACCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCGGTGC > minE/2584754‑2584888 | aaaTTTCATGGCGTCACCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGt > 1:871210/1‑71 (MQ=255)aaaTTTCATGGCGTCACCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGt > 1:1591199/1‑71 (MQ=255) aaTTTCATGGCGTCACCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGtt < 1:291906/71‑1 (MQ=255) aaTTTCATGGCGTCACCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGtt > 1:46264/1‑71 (MQ=255) cATGGCGTCACCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGc > 1:3851903/1‑71 (MQ=255) cATGGCGTCACCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGc > 1:3856223/1‑71 (MQ=255) gCGTCACCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTc < 1:476602/71‑1 (MQ=255) tCACCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCg < 1:3469748/57‑1 (MQ=255) aCCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTg > 1:2064563/1‑58 (MQ=255) aCCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTa > 1:3543763/1‑60 (MQ=255) cGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTcgc < 1:1214200/71‑1 (MQ=255) ggTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTcgcg < 1:887341/71‑1 (MQ=255) ttttCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGt < 1:1448764/51‑1 (MQ=255) ttttCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTa < 1:322310/52‑1 (MQ=255) tttCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAAc > 1:2404640/1‑71 (MQ=255) tttCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAAc > 1:566124/1‑71 (MQ=255) ttCCCGGCCGCCGCTTCACCTAAAATCTCCATTGATGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAAc > 1:1982648/1‑70 (MQ=255) ttCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAg > 1:3266592/1‑59 (MQ=255) ttCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGaa < 1:2093379/69‑1 (MQ=255) gccgcTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCa < 1:3766289/71‑1 (MQ=255) cTTCACCTAAAATCTCCATTGACGGGATGCCGTTg < 1:3826346/35‑1 (MQ=255) tCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGt > 1:1408989/1‑51 (MQ=255) tCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAAc < 1:1954103/55‑1 (MQ=255) aCCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTa < 1:438634/70‑1 (MQ=255) cTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATa > 1:3111644/1‑55 (MQ=255) tAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGt > 1:2109649/1‑46 (MQ=255) tAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCa < 1:2430727/52‑1 (MQ=255) tAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCa < 1:1076991/60‑1 (MQ=255) tAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGt > 1:2330216/1‑66 (MQ=255) aaaaTCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGAc > 1:3488731/1‑69 (MQ=255) aaaTCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAAc < 1:1051946/48‑1 (MQ=255) aaaTCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAg > 1:1176556/1‑53 (MQ=255) aaaTCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGa < 1:932127/70‑1 (MQ=255) aaaTCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAg > 1:1196257/1‑71 (MQ=255) aaaTCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAg > 1:328266/1‑71 (MQ=255) aaTCTCCATTGACGGCAGACCGTTGTAGCGTTCCAGTcgcg < 1:1413110/41‑1 (MQ=37) aTCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAAc > 1:316198/1‑46 (MQ=255) tctcCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAg > 1:1243760/1‑50 (MQ=255) ctcCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACg < 1:2841894/65‑1 (MQ=255) tcCATTGACGGGATGCCGTTGTAGGGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAgg > 1:626195/1‑70 (MQ=255) tcCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCAt < 1:3789759/46‑1 (MQ=255) cATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGAc < 1:3869215/61‑1 (MQ=255) cATTGACGGCAGACCGTTGTAGCGTTCCAGTCGCGGAGAGCCATac > 1:855210/1‑45 (MQ=255) aatGACGGGATGCCGTTGAAGCGTTCCAGTCGCGGTGAACCATACGTCCATTCGGTAGACg > 1:908317/3‑61 (MQ=255) ttGACGGTAGACCGTTGTAGCGTTCCAGTCGCGGAGAGCCa < 1:493807/41‑1 (MQ=255) ttGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCAt < 1:3410269/42‑1 (MQ=255) ttGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAg < 1:3431801/69‑1 (MQ=255) ttGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAg < 1:3384800/69‑1 (MQ=255) ttGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGaa < 1:1562975/71‑1 (MQ=255) ttGACGGCAGACCGTTGTAGCGTTCCAGTCGCGGAGAGCCa < 1:2176676/41‑1 (MQ=255) ttGACGGCAGACCGTTGTAGCGTTCCAGTCGCGGAGAGCCa < 1:3305448/41‑1 (MQ=255) tGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGa > 1:13702/1‑69 (MQ=255) gACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATa > 1:1388946/1‑41 (MQ=255) gACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGaaa > 1:379974/1‑70 (MQ=255) gACGGGATACCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGa > 1:3422552/1‑59 (MQ=255) aCGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAg < 1:2937752/70‑1 (MQ=255) aCGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAg < 1:3825307/70‑1 (MQ=255) cGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCg > 1:1764679/1‑71 (MQ=255) cGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCg > 1:402288/1‑71 (MQ=255) cGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCg > 1:2158479/1‑71 (MQ=255) cGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCg > 1:2572199/1‑71 (MQ=255) gggATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTa < 1:3721744/51‑1 (MQ=255) gATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCGGTg > 1:3675228/1‑71 (MQ=255) aTGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCg < 1:2459784/67‑1 (MQ=255) aTGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCg < 1:2960790/67‑1 (MQ=255) aTGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCGGTGc < 1:1714575/71‑1 (MQ=255) aTGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCGGTGc > 1:1827325/1‑71 (MQ=255) tGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGc > 1:3750150/1‑58 (MQ=255) tGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCGGTGc > 1:2922522/1‑70 (MQ=255) gCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGa < 1:334843/60‑1 (MQ=255) gCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGa < 1:10468/60‑1 (MQ=255) | AAATTTCATGGCGTCACCGGTACTTTTCCCGGCCGCCGCTTCACCTAAAATCTCCATTGACGGGATGCCGTTGTAGCGTTCCAGTCGCGGTGAACCATAGGTCCATTCGGTAGACGAGTAGGCAGAAAGCGGTGC > minE/2584754‑2584888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |