| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,586,017 | A→C | 9.2% | I420M (ATT→ATG) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,586,017 | 0 | A | C | 9.2% | 271.4 / 23.6 | 109 | I420M (ATT→ATG) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (54/45); new base C (2/8); total (56/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
ACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATC > minE/2585949‑2586086 | aCCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAAt < 1:3550423/71‑1 (MQ=255)aCCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAAt < 1:412882/71‑1 (MQ=255) cgcACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGacac < 1:3478591/71‑1 (MQ=255) cgcACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGacac < 1:2927509/71‑1 (MQ=255) gcACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGa > 1:3380668/1‑67 (MQ=255) cACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGa > 1:818546/1‑66 (MQ=255) gTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTc < 1:3039609/70‑1 (MQ=255) tGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGAc > 1:1116533/1‑71 (MQ=255) ggATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGAc < 1:148213/70‑1 (MQ=255) ggATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACg > 1:3187793/1‑71 (MQ=255) ggATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACg > 1:284226/1‑71 (MQ=255) ggATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTAACTTCCGCAATGACACGCTCGACg > 1:86847/1‑71 (MQ=255) tCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCaa > 1:3157120/1‑54 (MQ=255) tGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtctc > 1:2822200/1‑71 (MQ=255) tGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtctc > 1:3710093/1‑71 (MQ=255) tGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtctc > 1:2667679/1‑71 (MQ=255) tGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtctc > 1:1540611/1‑71 (MQ=255) gCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtctc > 1:2567313/1‑70 (MQ=255) cgacATCGATTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTcca < 1:3377/68‑1 (MQ=255) ccccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtctc < 1:2347520/69‑1 (MQ=255) acATCGATTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTccacc < 1:795172/70‑1 (MQ=255) aTCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccac > 1:104508/1‑71 (MQ=255) aTCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccac < 1:2292232/71‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGa < 1:2468640/48‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGAGACGCTCGACGTTCTccaccac > 1:646283/1‑70 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccacc < 1:3466649/71‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccac > 1:781318/1‑70 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccac < 1:1987355/67‑1 (MQ=255) cGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATg < 1:3677992/46‑1 (MQ=255) cGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGAAATGACACGCTCGACGTTCTccaccacca > 1:3596741/1‑71 (MQ=255) gATTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTccaccacca > 1:1034524/1‑70 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGacac > 1:1658697/1‑49 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGa > 1:3665277/1‑46 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGa > 1:1817004/1‑46 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccacca > 1:2664385/1‑70 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTcc > 1:3998611/1‑63 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:3083339/1‑71 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:2975242/1‑71 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac < 1:3123705/71‑1 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:2474361/1‑71 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:3514972/1‑71 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:2179504/1‑71 (MQ=255) gATTTATGCGTAGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac < 1:4086823/71‑1 (MQ=255) aTTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGa < 1:2230086/54‑1 (MQ=255) aTTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGa < 1:3385717/54‑1 (MQ=255) aTTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac < 1:2636548/70‑1 (MQ=255) aTTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac < 1:2686758/70‑1 (MQ=255) tttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGtt > 1:1567068/1‑57 (MQ=255) tttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa > 1:2428704/1‑71 (MQ=255) aTGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATg < 1:3102437/70‑1 (MQ=255) tGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTcca > 1:932963/1‑58 (MQ=255) cGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCg < 1:2174545/70‑1 (MQ=255) tCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATTACACGCTCGACGTTCTCCACCACCACGATGGCGTCa > 1:3425377/1‑71 (MQ=255) tCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCa > 1:548634/1‑71 (MQ=255) gCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATc > 1:2163682/1‑71 (MQ=255) cttcttttGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCatcatc < 1:3797960/70‑1 (MQ=12) ccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:1032638/70‑1 (MQ=255) ccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:3623113/70‑1 (MQ=255) ccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac > 1:2138510/1‑70 (MQ=255) cggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGt < 1:2144167/70‑1 (MQ=255) ggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTaa < 1:2388022/71‑1 (MQ=255) gcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc < 1:3349906/71‑1 (MQ=255) gggaGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGccc < 1:3071193/68‑1 (MQ=9) gggaGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGccc < 1:626892/68‑1 (MQ=9) gTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa < 1:661694/48‑1 (MQ=255) gTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAccc > 1:790588/1‑70 (MQ=255) tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCCCCAGTAACCCTa > 1:1636305/1‑71 (MQ=255) tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCt < 1:52052/70‑1 (MQ=255) tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTa < 1:3260103/71‑1 (MQ=255) tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTa > 1:767643/1‑71 (MQ=255) gaGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGcccga < 1:4053857/70‑3 (MQ=255) aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAg > 1:1719769/1‑62 (MQ=255) aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCt > 1:4113399/1‑69 (MQ=255) aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTAt > 1:1670558/1‑71 (MQ=255) gCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccac > 1:1035014/1‑40 (MQ=255) gCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCGCGATGGCGTCATcca > 1:1881326/1‑57 (MQ=255) gCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcca > 1:1924630/1‑57 (MQ=255) gCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcca > 1:579385/1‑57 (MQ=255) cTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc < 1:1695424/64‑1 (MQ=255) cTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCatcatcg < 1:3896454/55‑2 (MQ=255) cTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCatcatcg < 1:3377221/55‑2 (MQ=255) tATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc > 1:920585/1‑70 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCCCCACGATGGCGTCATccacca > 1:837820/1‑56 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGc < 1:2556031/45‑1 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGc < 1:176843/45‑1 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGc < 1:3594923/45‑1 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGt > 1:1107565/1‑58 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc > 1:1404749/1‑69 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc > 1:899383/1‑69 (MQ=255) tCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCt < 1:3825497/63‑1 (MQ=255) cTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGt > 1:4047199/1‑45 (MQ=255) cTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg > 1:2156977/1‑70 (MQ=255) ttCCGCAATGACACGCTCGACGTTCTccaccacct > 1:1109879/1‑34 (MQ=255) ttCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATc < 1:562592/48‑1 (MQ=255) ttCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCa > 1:3505798/1‑71 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg < 1:1017283/67‑1 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg < 1:2466172/67‑1 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCa < 1:2726256/69‑1 (MQ=255) cAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATc < 1:2465905/71‑1 (MQ=255) aaTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATc < 1:644711/70‑1 (MQ=255) | ACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATC > minE/2585949‑2586086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |