| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,586,065 | T→C | 6.7% | L404L (TTA→TTG) ‡ | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,586,065 | 0 | T | C | 6.7% | 297.8 / 10.7 | 104 | L404L (TTA→TTG) ‡ | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (41/56); new base C (3/4); total (44/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
Only 100 of 112 total aligned reads displayed.
CGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGAT > minE/2585996‑2586133 | cggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGt < 1:2144167/70‑1 (MQ=255) gcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc < 1:3349906/71‑1 (MQ=255) gggaGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGccc < 1:3071193/68‑1 (MQ=9) gggaGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGccc < 1:626892/68‑1 (MQ=9) gTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAccc > 1:790588/1‑70 (MQ=255) tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCCCCAGTAACCCTa > 1:1636305/1‑71 (MQ=255) tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCt < 1:52052/70‑1 (MQ=255) tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTa < 1:3260103/71‑1 (MQ=255) gaGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGcccga < 1:4053857/70‑3 (MQ=255) gaGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGcccga > 1:222344/2‑69 (MQ=255) aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCt > 1:4113399/1‑69 (MQ=255) aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTAt > 1:1670558/1‑71 (MQ=255) cTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc < 1:1695424/64‑1 (MQ=255) tATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc > 1:920585/1‑70 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGt > 1:1107565/1‑58 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc > 1:899383/1‑69 (MQ=255) tCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCt < 1:3825497/63‑1 (MQ=255) cTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg > 1:2156977/1‑70 (MQ=255) ttCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCa > 1:3505798/1‑71 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg < 1:1017283/67‑1 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg < 1:2466172/67‑1 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCa < 1:2726256/69‑1 (MQ=255) cAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCAt < 1:3209622/70‑1 (MQ=255) cAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATc < 1:2465905/71‑1 (MQ=255) aaTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATc < 1:644711/70‑1 (MQ=255) aTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCt < 1:1102717/54‑1 (MQ=255) aTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCa < 1:3473991/67‑1 (MQ=255) aTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCa < 1:1663049/67‑1 (MQ=255) tGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATccc > 1:1348189/1‑70 (MQ=255) tGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCg < 1:814906/71‑1 (MQ=255) acgcGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGaa > 1:2630334/4‑71 (MQ=2) acacGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:3560893/1‑62 (MQ=255) acacGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGaa > 1:501919/1‑71 (MQ=255) acacGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGaa > 1:3412148/1‑71 (MQ=255) acGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa < 1:3927935/71‑1 (MQ=255) tCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAAc < 1:379082/66‑1 (MQ=9) cGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGt > 1:3853016/1‑70 (MQ=2) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc < 1:2081560/70‑1 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc < 1:1334357/70‑1 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc < 1:3155042/70‑1 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCa < 1:115142/71‑1 (MQ=255) gACGTTCTCCAACACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc < 1:1914800/70‑1 (MQ=255) ttCTCCACCACCACGATGGCGTCATCCACCAGTAAccc < 1:1429061/38‑1 (MQ=255) ttCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATa < 1:920801/63‑1 (MQ=255) tctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATcc > 1:4138693/1‑54 (MQ=255) tctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATcc > 1:932832/1‑54 (MQ=255) ctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAGCATACCGAACATAGTCAACgtgt > 1:3743712/1‑71 (MQ=255) ctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAAc > 1:3828203/1‑67 (MQ=255) ctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAAc > 1:2643675/1‑67 (MQ=255) ctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgtg < 1:2930515/70‑1 (MQ=255) caccaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGtt < 1:2970651/69‑1 (MQ=255) caccaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTg < 1:1637659/70‑1 (MQ=255) caccaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGa < 1:667836/71‑1 (MQ=255) accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGAt < 1:1880151/71‑1 (MQ=255) ccaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa < 1:3462353/54‑1 (MQ=255) ccaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCa < 1:591886/60‑1 (MQ=255) caccacGATTGCGTCATCCACAAGTAACCCTATCGCCAGCACCATCCCGAACa < 1:1477405/53‑1 (MQ=21) accacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGa > 1:1766881/1‑67 (MQ=255) ccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGa > 1:4011644/1‑66 (MQ=255) ccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTg < 1:3833700/71‑1 (MQ=255) cacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTg > 1:1881580/1‑70 (MQ=255) cacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGa < 1:2403952/71‑1 (MQ=255) cacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGa < 1:1635053/71‑1 (MQ=255) acGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGa > 1:2046148/1‑70 (MQ=255) acGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGa > 1:3312876/1‑70 (MQ=255) acGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACAATCCCGAACATAGTCAACGTGTTGATGGTGa > 1:3819739/1‑70 (MQ=255) acGATGGCGTCATCCACCACTAACCCTATCGACAGCAAGATCCCGAACATAGTCAACGTGTAGAAGGt > 1:924041/1‑68 (MQ=25) tGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaa < 1:35588/67‑1 (MQ=255) tGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaa < 1:3964587/67‑1 (MQ=255) tGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAacc > 1:2920476/1‑70 (MQ=255) gCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAaccgac > 1:3746943/1‑71 (MQ=255) cGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAAc > 1:4051349/1‑50 (MQ=255) cGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAaccgacc > 1:1517052/1‑71 (MQ=255) cGTCAT‑CACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAaccgacc < 1:3706088/70‑1 (MQ=255) tCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGtt < 1:2191374/53‑1 (MQ=255) cATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGAccgcc > 1:1595167/1‑71 (MQ=255) cATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGAccgcc > 1:2909912/1‑71 (MQ=255) aTCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAaccgac > 1:338247/1‑66 (MQ=255) aTCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGAccgcc > 1:3148884/1‑70 (MQ=255) tCCACCAGTAACCCTATCGCTAGCACCATCCCGAACATAGTc < 1:2491892/42‑1 (MQ=255) tCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgtg < 1:2125051/48‑1 (MQ=255) accaGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACaa < 1:1224088/71‑1 (MQ=255) ccaGTAACCCTATCGCCAGCACCATCCCGAACATa < 1:3934213/35‑1 (MQ=255) ccaGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACGGCCGACAAg < 1:213206/71‑1 (MQ=255) ccaGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAg < 1:3971846/71‑1 (MQ=255) caGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGa < 1:954895/71‑1 (MQ=255) caGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGa < 1:2940710/71‑1 (MQ=255) aGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAAc > 1:2026449/1‑39 (MQ=255) aGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAAc > 1:1037108/1‑39 (MQ=255) aGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTg > 1:2995348/1‑51 (MQ=255) aGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTg > 1:1754906/1‑51 (MQ=255) aGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGAc > 1:2816409/1‑66 (MQ=255) aGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGAc > 1:1342981/1‑66 (MQ=255) aGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGAt < 1:3074936/71‑1 (MQ=255) gTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGa < 1:3167683/45‑1 (MQ=255) tAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGtt < 1:3786760/42‑1 (MQ=255) tAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGtt < 1:1538144/42‑1 (MQ=255) tAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTg > 1:3734539/1‑49 (MQ=255) tAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACaa < 1:3942450/66‑1 (MQ=255) tAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGa > 1:3215870/1‑68 (MQ=255) | CGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGAT > minE/2585996‑2586133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |