| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,319 | A→C | 15.7% | F427V (TTC→GTC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,319 | 0 | A | C | 15.7% | 194.3 / 48.7 | 102 | F427V (TTC→GTC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base A (42/44); new base C (10/6); total (52/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
CGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGG > minE/2595249‑2595389 | cgcgACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGaa < 1:2880073/71‑1 (MQ=255)cgcgACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGaa < 1:2384280/71‑1 (MQ=255) gcgACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGaaa < 1:2336324/71‑1 (MQ=255) gACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAaga < 1:885400/71‑1 (MQ=255) gACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAaga < 1:77634/71‑1 (MQ=255) ccGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAagaga > 1:3346957/1‑67 (MQ=255) ccGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAagaga < 1:3826643/67‑1 (MQ=255) ccGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACg < 1:2828871/71‑1 (MQ=255) cGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAag > 1:2236511/1‑63 (MQ=255) cGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAag > 1:1790879/1‑63 (MQ=255) cAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACagagagc > 1:1754559/1‑65 (MQ=255) cAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGaaa > 1:2392040/1‑60 (MQ=255) cAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGaaa > 1:1723584/1‑60 (MQ=255) ccGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGcc < 1:1336311/71‑1 (MQ=255) cGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAgcgc > 1:3884315/1‑69 (MQ=255) tgtgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAgagaga < 1:1168801/56‑1 (MQ=255) tgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGcc < 1:2358810/63‑1 (MQ=255) tgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcag < 1:1479919/70‑1 (MQ=255) aTAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCa > 1:3451772/1‑62 (MQ=255) aTAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc > 1:31842/1‑69 (MQ=255) agaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca > 1:1307160/3‑69 (MQ=255) gaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccagag > 1:417408/2‑69 (MQ=255) aaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCg > 1:2351324/1‑71 (MQ=255) aaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCg < 1:3550584/71‑1 (MQ=255) aaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCg > 1:1405204/1‑71 (MQ=255) ggTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTca > 1:4039555/1‑71 (MQ=255) ggTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTca > 1:4030391/1‑71 (MQ=255) gTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgcgc < 1:831615/59‑1 (MQ=255) tAATGTTGCTCATATCAGTATCTAATTACAAAGTGACaga > 1:3896011/1‑40 (MQ=38) tAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTgaca < 1:1124709/71‑1 (MQ=255) tAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTgaca < 1:873995/71‑1 (MQ=255) aaTGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAg > 1:1842547/1‑48 (MQ=255) aTGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGt < 1:607554/45‑1 (MQ=255) tGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGCGACGTAGCGCCAGCGCAGCCAGCGTCACAAAc < 1:666367/71‑1 (MQ=255) tGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCCGCGCGGCCAGAGt < 1:4037393/64‑1 (MQ=255) gTTGCTCATATCAGTATCTCATTACAAAGTGAAagaga > 1:1130018/1‑38 (MQ=255) gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa > 1:1021749/1‑69 (MQ=255) ttGCTCATATCAGTATCTCATTACAAAGTGAAagaga > 1:2393771/1‑37 (MQ=255) ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg < 1:2015386/71‑1 (MQ=255) ttGCTCATATCAGTATCTAATTACAAAGTGACaga > 1:836820/1‑35 (MQ=37) ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCgc > 1:2944192/1‑41 (MQ=37) ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACaaa > 1:2576625/1‑68 (MQ=255) ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa < 1:3694442/70‑1 (MQ=255) ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAg > 1:2906983/1‑71 (MQ=255) atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg > 1:3357550/1‑71 (MQ=255) tatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggc < 1:3069705/71‑1 (MQ=255) tatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg > 1:2013140/1‑70 (MQ=255) tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag > 1:2561007/1‑71 (MQ=255) tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag > 1:1528910/1‑71 (MQ=255) tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag > 1:1521584/1‑71 (MQ=255) tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag < 1:1319623/71‑1 (MQ=255) aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc > 1:3974419/1‑52 (MQ=255) gTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc < 1:2570829/48‑1 (MQ=255) tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTc < 1:3517368/71‑1 (MQ=255) tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTc < 1:1207333/71‑1 (MQ=255) aTCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCa > 1:1475760/1‑71 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGcaca < 1:21487/39‑1 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc > 1:4059495/1‑41 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGca < 1:3236470/39‑1 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt < 1:3392721/47‑1 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt < 1:289700/47‑1 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca < 1:94865/51‑1 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca < 1:2182550/51‑1 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt > 1:2220851/1‑71 (MQ=255) tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt > 1:1143955/1‑71 (MQ=255) ctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa > 1:581078/1‑52 (MQ=255) tcATTACATAGTGAAAGAGATACGTAGCGCCAGCGCAGCCAGCGTcaca < 1:1696701/49‑1 (MQ=38) tcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca < 1:1005006/49‑1 (MQ=255) tcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca < 1:1323027/49‑1 (MQ=255) tcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca < 1:1005448/49‑1 (MQ=255) tcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa > 1:87160/1‑71 (MQ=255) cATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg > 1:3475973/1‑69 (MQ=255) cATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg > 1:3463881/1‑69 (MQ=255) aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt < 1:427955/67‑1 (MQ=255) aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt > 1:1450432/1‑70 (MQ=255) aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa < 1:14396/71‑1 (MQ=255) ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGcag < 1:612322/35‑1 (MQ=255) ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGcag < 1:4088868/35‑1 (MQ=255) ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt < 1:3947624/42‑1 (MQ=255) tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca < 1:3973420/45‑1 (MQ=255) tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt > 1:1778553/1‑71 (MQ=255) aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg < 1:1522056/59‑1 (MQ=255) aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa > 1:2419528/1‑66 (MQ=255) aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc > 1:1923899/1‑71 (MQ=255) aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc > 1:2465341/1‑71 (MQ=255) aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATccc < 1:3610673/71‑1 (MQ=255) aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATccc < 1:1333661/71‑1 (MQ=255) aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca < 1:3864270/41‑1 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt > 1:4029097/1‑36 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCa > 1:741238/1‑59 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:3023071/71‑1 (MQ=255) gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg > 1:1961298/1‑70 (MQ=255) gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg > 1:2602856/1‑70 (MQ=255) aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcag > 1:915525/1‑46 (MQ=255) aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg < 1:3317553/57‑1 (MQ=255) aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa > 1:1269738/1‑58 (MQ=255) aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACg > 1:2924354/1‑71 (MQ=255) aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:3705634/71‑1 (MQ=255) aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:1728026/71‑1 (MQ=255) aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:2684986/71‑1 (MQ=255) | CGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGG > minE/2595249‑2595389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |