Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,341 A→G 7.9% A419A (GCT→GCC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,3410AG 7.9% 284.4 / 12.9 101A419A (GCT→GCCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (49/44);  new base G (3/5);  total (52/49)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGA  >  minE/2595273‑2595408
                                                                    |                                                                   
tgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcag                                                                    <  1:1479919/70‑1 (MQ=255)
    agaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                                 >  1:1307160/3‑69 (MQ=255)
     gaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccagag                                                              >  1:417408/2‑69 (MQ=255)
     aaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCg                                                              >  1:2351324/1‑71 (MQ=255)
     aaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCg                                                              <  1:3550584/71‑1 (MQ=255)
     aaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCg                                                              >  1:1405204/1‑71 (MQ=255)
        ggTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTca                                                           >  1:4039555/1‑71 (MQ=255)
        ggTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTca                                                           >  1:4030391/1‑71 (MQ=255)
          tAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTgaca                                                         <  1:873995/71‑1 (MQ=255)
          tAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTgaca                                                         <  1:1124709/71‑1 (MQ=255)
             tGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGCGACGTAGCGCCAGCGCAGCCAGCGTCACAAAc                                                      <  1:666367/71‑1 (MQ=255)
             tGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCCGCGCGGCCAGAGt                                                             <  1:4037393/64‑1 (MQ=255)
              gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa                                                       >  1:1021749/1‑69 (MQ=255)
               ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACaaa                                                       >  1:2576625/1‑68 (MQ=255)
               ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                                     <  1:3694442/70‑1 (MQ=255)
               ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAg                                                    >  1:2906983/1‑71 (MQ=255)
                     atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg                                              >  1:3357550/1‑71 (MQ=255)
                      tatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggc                                             <  1:3069705/71‑1 (MQ=255)
                      tatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg                                              >  1:2013140/1‑70 (MQ=255)
                        tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                           >  1:1521584/1‑71 (MQ=255)
                        tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                           >  1:1528910/1‑71 (MQ=255)
                        tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                           >  1:2561007/1‑71 (MQ=255)
                          aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                                            >  1:3974419/1‑52 (MQ=255)
                           gTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                               <  1:2570829/48‑1 (MQ=255)
                            tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTc                                       <  1:1207333/71‑1 (MQ=255)
                            tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTc                                       <  1:3517368/71‑1 (MQ=255)
                             aTCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCa                                      >  1:1475760/1‑71 (MQ=255)
                              tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGcaca                                                                     <  1:21487/39‑1 (MQ=255)
                              tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                                   >  1:4059495/1‑41 (MQ=255)
                              tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGca                                                                     <  1:3236470/39‑1 (MQ=255)
                              tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                                             <  1:289700/47‑1 (MQ=255)
                              tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                                             <  1:3392721/47‑1 (MQ=255)
                              tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                                         <  1:2182550/51‑1 (MQ=255)
                              tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                                         <  1:94865/51‑1 (MQ=255)
                              tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                                            >  1:961741/1‑48 (MQ=255)
                              tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                     >  1:2220851/1‑71 (MQ=255)
                              tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                     >  1:1229331/1‑71 (MQ=255)
                              tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                                     >  1:1143955/1‑71 (MQ=255)
                               ctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa                                                       >  1:581078/1‑52 (MQ=255)
                                tcATTACATAGTGAAAGAGATACGTAGCGCCAGCGCAGCCAGCGTcaca                                                         <  1:1696701/49‑1 (MQ=38)
                                tcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                                         <  1:1005448/49‑1 (MQ=255)
                                tcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                                         <  1:1005006/49‑1 (MQ=255)
                                tcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                                         <  1:1323027/49‑1 (MQ=255)
                                tcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                   >  1:87160/1‑71 (MQ=255)
                                tcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                   >  1:1610239/1‑71 (MQ=255)
                                 cATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg                                         >  1:3077170/1‑64 (MQ=255)
                                 cATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg                                    >  1:3463881/1‑69 (MQ=255)
                                 cATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg                                    >  1:3475973/1‑69 (MQ=255)
                                  aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                                  <  1:2803491/70‑2 (MQ=255)
                                  aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                     <  1:427955/67‑1 (MQ=255)
                                  aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt                                  >  1:1450432/1‑70 (MQ=255)
                                  aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                                 <  1:14396/71‑1 (MQ=255)
                                   ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGcag                                                                    <  1:4088868/35‑1 (MQ=255)
                                   ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGcag                                                                    <  1:612322/35‑1 (MQ=255)
                                   ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                                             <  1:3947624/42‑1 (MQ=255)
                                    tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                                         <  1:3973420/45‑1 (MQ=255)
                                    tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                           <  1:4028717/59‑1 (MQ=255)
                                    tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                               >  1:1778553/1‑71 (MQ=255)
                                     aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                   >  1:2419528/1‑66 (MQ=255)
                                     aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                              >  1:1923899/1‑71 (MQ=255)
                                     aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                              >  1:2465341/1‑71 (MQ=255)
                                       aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATccc                            <  1:1333661/71‑1 (MQ=255)
                                       aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATccc                            <  1:3610673/71‑1 (MQ=255)
                                         aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                                             >  1:4029097/1‑36 (MQ=255)
                                         aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCa                                      >  1:741238/1‑59 (MQ=255)
                                         aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                          <  1:3023071/71‑1 (MQ=255)
                                          gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                          >  1:2408914/1‑70 (MQ=255)
                                          gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                          >  1:1961298/1‑70 (MQ=255)
                                          gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                          >  1:2602856/1‑70 (MQ=255)
                                            gAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTgatgat                                  <  1:1408265/60‑1 (MQ=255)
                                             aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcag                                               >  1:915525/1‑46 (MQ=255)
                                             aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg                                    <  1:3317553/57‑1 (MQ=255)
                                             aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                   >  1:1269738/1‑58 (MQ=255)
                                             aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACg                      >  1:2924354/1‑71 (MQ=255)
                                              aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                     <  1:1728026/71‑1 (MQ=255)
                                              aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                     <  1:3705634/71‑1 (MQ=255)
                                              aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                     <  1:2684986/71‑1 (MQ=255)
                                               agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                     >  1:2619540/1‑70 (MQ=255)
                                               agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                     >  1:1833044/1‑70 (MQ=255)
                                               agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                     >  1:3004337/1‑70 (MQ=255)
                                               agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGa                    <  1:3008348/71‑1 (MQ=255)
                                                  gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCa                                      >  1:3803614/1‑50 (MQ=255)
                                                   agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg                  >  1:821700/1‑69 (MQ=255)
                                                   agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg                  >  1:816757/1‑69 (MQ=255)
                                                   agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                <  1:3709359/71‑1 (MQ=255)
                                                    gaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa               <  1:3628161/71‑1 (MQ=255)
                                                    gaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa               >  1:3399759/1‑71 (MQ=255)
                                                    gaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa               <  1:778476/71‑1 (MQ=255)
                                                     aCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa               >  1:537398/1‑70 (MQ=255)
                                                        tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt                                  >  1:78461/1‑48 (MQ=255)
                                                        tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAata             >  1:2637145/1‑69 (MQ=255)
                                                         aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                                >  1:3272811/1‑49 (MQ=255)
                                                          gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc         >  1:2700483/1‑71 (MQ=255)
                                                           cgcCTGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGt                                        <  1:2854584/39‑1 (MQ=255)
                                                             ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                   <  1:2345536/42‑1 (MQ=255)
                                                             ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                   <  1:3652998/42‑1 (MQ=255)
                                                             ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa               <  1:631034/62‑1 (MQ=255)
                                                               aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATACCCAGCt    <  1:2398680/71‑1 (MQ=255)
                                                                  gcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa  >  1:345183/1‑70 (MQ=255)
                                                                  gcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa  >  1:1051765/1‑70 (MQ=255)
                                                                    |                                                                   
TGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGA  >  minE/2595273‑2595408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: