| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,376 | T→C | 6.7% | I408V (ATC→GTC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,376 | 0 | T | C | 6.7% | 258.2 / 7.9 | 89 | I408V (ATC→GTC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base T (41/42); new base C (2/4); total (43/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGT > minE/2595307‑2595446 | aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt > 1:1450432/1‑70 (MQ=255)aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa < 1:14396/71‑1 (MQ=255) tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt > 1:1778553/1‑71 (MQ=255) aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc > 1:2465341/1‑71 (MQ=255) aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc > 1:1923899/1‑71 (MQ=255) aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATccc < 1:3610673/71‑1 (MQ=255) aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATccc < 1:1333661/71‑1 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:3023071/71‑1 (MQ=255) gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg > 1:2408914/1‑70 (MQ=255) gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg > 1:1961298/1‑70 (MQ=255) gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg > 1:2602856/1‑70 (MQ=255) gAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTgatgat < 1:1408265/60‑1 (MQ=255) aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACg > 1:2924354/1‑71 (MQ=255) aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:1728026/71‑1 (MQ=255) aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:3705634/71‑1 (MQ=255) aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:2684986/71‑1 (MQ=255) agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg > 1:3004337/1‑70 (MQ=255) agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg > 1:1833044/1‑70 (MQ=255) agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg > 1:2619540/1‑70 (MQ=255) agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGa < 1:3008348/71‑1 (MQ=255) agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg > 1:816757/1‑69 (MQ=255) agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg > 1:821700/1‑69 (MQ=255) agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa < 1:3709359/71‑1 (MQ=255) gaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa < 1:3628161/71‑1 (MQ=255) gaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa < 1:778476/71‑1 (MQ=255) gaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa > 1:3399759/1‑71 (MQ=255) aCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa > 1:537398/1‑70 (MQ=255) tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt > 1:78461/1‑48 (MQ=255) tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAata > 1:2637145/1‑69 (MQ=255) aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa > 1:3272811/1‑49 (MQ=255) aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc > 1:2510901/1‑52 (MQ=255) gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:2700483/1‑71 (MQ=255) gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc < 1:75801/70‑1 (MQ=255) ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa < 1:631034/62‑1 (MQ=255) aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt < 1:3509221/71‑1 (MQ=255) aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATACCCAGCt < 1:2398680/71‑1 (MQ=255) gcgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTAc < 1:4074871/51‑1 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa < 1:2804827/58‑1 (MQ=255) gcggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAt > 1:304701/4‑71 (MQ=255) gcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa > 1:1051765/1‑70 (MQ=255) gcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa > 1:345183/1‑70 (MQ=255) gccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc < 1:849417/61‑1 (MQ=255) ccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTg < 1:3610481/65‑1 (MQ=255) cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa > 1:542016/1‑71 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:2152524/41‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:394732/41‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:1036500/41‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa > 1:3783589/1‑43 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg < 1:2691768/44‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg < 1:84994/44‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt < 1:1568847/45‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt < 1:3844983/45‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa < 1:3121005/47‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc < 1:561201/71‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc < 1:1822834/71‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc > 1:2031137/1‑71 (MQ=255) cacaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt < 1:849909/44‑1 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACg > 1:1060898/1‑38 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat > 1:3633580/1‑46 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCa > 1:3681957/1‑53 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTg < 1:1752835/57‑1 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTg < 1:484389/57‑1 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:2506286/1‑71 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:2165363/1‑71 (MQ=255) caAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc < 1:1889340/71‑1 (MQ=255) caAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc > 1:2525046/1‑71 (MQ=255) caAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc < 1:2508316/71‑1 (MQ=255) gCACAGGCAGGGTCATGTTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa < 1:3516601/71‑1 (MQ=255) acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg < 1:1201508/71‑1 (MQ=255) caggcaggGTCATGATAATCCCTGTACGGAAGTAata < 1:348770/37‑1 (MQ=255) caggcaggGTCATGATAATCCCTGTACGGAAGTAata < 1:32898/37‑1 (MQ=255) ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc < 1:848417/51‑1 (MQ=255) ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc < 1:3288447/51‑1 (MQ=255) gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg > 1:330065/1‑71 (MQ=255) gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg > 1:51357/1‑71 (MQ=255) gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc < 1:974816/56‑1 (MQ=255) ggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTg > 1:76264/1‑69 (MQ=255) tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGc < 1:770669/71‑1 (MQ=255) cATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc > 1:3696913/1‑70 (MQ=255) cATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc > 1:2458076/1‑70 (MQ=255) cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt < 1:315477/51‑1 (MQ=255) aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc > 1:2529828/1‑69 (MQ=255) aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa < 1:3659215/71‑1 (MQ=255) aTGATAATCACTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa > 1:4099395/1‑61 (MQ=255) aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa > 1:1819255/1‑71 (MQ=255) aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa < 1:3072840/71‑1 (MQ=255) tGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCag < 1:2091029/71‑1 (MQ=255) gATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCat > 1:1226182/1‑42 (MQ=255) gATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa > 1:2284123/1‑69 (MQ=255) gATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa > 1:2359636/1‑69 (MQ=255) aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag > 1:1817348/1‑71 (MQ=255) aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa > 1:2737528/1‑68 (MQ=255) tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgt < 1:528847/71‑1 (MQ=255) | ATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGT > minE/2595307‑2595446 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |