| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 165,160 | A→G | 15.5% | F772F (TTT→TTC) | glnD ← | uridylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 165,160 | 0 | A | G | 15.5% | 207.2 / 29.4 | 84 | F772F (TTT→TTC) | glnD | uridylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (45/26); new base G (8/5); total (53/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TAATTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCC > minE/165091‑165229 | tAATTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCaa < 1:191152/71‑1 (MQ=255)tAATTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCaa < 1:1479125/71‑1 (MQ=255) aaTTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCgaa > 1:1999818/1‑71 (MQ=255) aaTTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCgaa > 1:2528351/1‑71 (MQ=255) aaTTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCaaa > 1:823044/1‑71 (MQ=255) aTTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAAc > 1:1043238/1‑71 (MQ=255) aTTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAAc > 1:1080932/1‑71 (MQ=255) tGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCg < 1:3705251/70‑1 (MQ=255) gggTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATaa < 1:1321850/71‑1 (MQ=255) ttGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCGAAcc > 1:1866039/1‑62 (MQ=255) gACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGa < 1:3512547/71‑1 (MQ=255) cGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACg < 1:3401362/71‑1 (MQ=255) ggggCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCGAAc > 1:2307930/1‑53 (MQ=255) ggggCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCGAACCGAATAACCTCATGACGa > 1:2473234/1‑71 (MQ=255) ggggCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTc < 1:163446/64‑1 (MQ=255) ggggCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACgc > 1:1158142/1‑70 (MQ=255) gggCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGaa < 1:3188378/56‑1 (MQ=255) cGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGc < 1:2178426/69‑1 (MQ=255) gCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGa < 1:9142/44‑1 (MQ=255) gCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGc > 1:788891/1‑71 (MQ=255) cTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGAt > 1:956412/1‑59 (MQ=255) cTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCg < 1:710533/71‑1 (MQ=255) gCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGa > 1:1404954/1‑64 (MQ=255) ccAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAAc < 1:3572076/45‑1 (MQ=255) ccAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCgg < 1:1195173/69‑1 (MQ=255) ccAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCgg < 1:2389576/69‑1 (MQ=255) cAGCTACTTTGCGTCAGTACTTGCTCCAGACCGAACCGAATAACCTCATGACGATCTGCGGACAGCg > 1:197932/1‑67 (MQ=255) cAGCTACCTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGtt < 1:4037525/71‑1 (MQ=255) gCTACTTTGCGTCAGTACTTGCTCCAGACCGAACCGAATAACCTCa > 1:4019877/1‑46 (MQ=255) gCTACTTTGCGTCAGTACTTGCTCCAGACCGAACCGAATAACCTCa > 1:3124682/1‑46 (MQ=255) cTACTTTGCGTCAGTACTTGCTCCAGACCGAACCg < 1:1978446/35‑1 (MQ=255) cTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGc > 1:1189604/1‑70 (MQ=255) tACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCa > 1:1086948/1‑71 (MQ=255) cTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAAcc < 1:3262625/39‑1 (MQ=255) tttGCGTCAGTACTTGCTCCAGACCGAACCGAATAACCTc < 1:4061944/40‑1 (MQ=255) tttGCGTCAGTACTTGCTCCAGACCAAACCGAATaa > 1:3875807/1‑36 (MQ=255) tttGCGTCAGTACTTGCTCCAGACCAAACCGAATaa > 1:1418816/1‑36 (MQ=255) ttGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCAt > 1:520477/1‑68 (MQ=255) ttGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCAt > 1:2405855/1‑68 (MQ=255) tGCGTCAGTACTTGCTCCAGACCGAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCggg > 1:829070/1‑71 (MQ=255) tGCGTCAGTACTTGCTCCAGACCGAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCg < 1:2024253/69‑1 (MQ=255) tGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCAt > 1:647517/1‑40 (MQ=255) tGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTg > 1:2296781/1‑49 (MQ=255) tGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCggg > 1:3716622/1‑71 (MQ=255) gCGTCAGTACTTGCTCCAGACCGAACCGAATAACCTCATg < 1:1501609/40‑1 (MQ=255) gCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCggg < 1:718976/70‑1 (MQ=255) gTCAGTACTTGCTCCAGACCGAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTc < 1:879939/71‑1 (MQ=255) aGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCgg > 1:2246532/1‑53 (MQ=255) aGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTc > 1:1314075/1‑68 (MQ=255) aGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTc > 1:1832863/1‑68 (MQ=255) aGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAg > 1:2871551/1‑71 (MQ=255) gTACTTGCTCCAGACCAAACCGAATAACCTCATGACg < 1:3519115/37‑1 (MQ=255) tACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCg < 1:1020182/43‑1 (MQ=255) tACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAg < 1:1954848/48‑1 (MQ=255) aCTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGtt > 1:104517/1‑53 (MQ=255) aCTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCAc > 1:2501692/1‑71 (MQ=255) aCTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCAc > 1:3526504/1‑71 (MQ=255) cTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCg > 1:912531/1‑48 (MQ=255) cTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGcc > 1:732501/1‑55 (MQ=255) cTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGcc > 1:3254840/1‑55 (MQ=255) cTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGcc > 1:2109353/1‑55 (MQ=255) cTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCAc > 1:1821098/1‑70 (MQ=255) ttGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGAc < 1:737705/43‑1 (MQ=255) tGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGa > 1:2356810/1‑70 (MQ=255) gCTCCAGACCGAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGAt > 1:110264/1‑70 (MQ=255) gCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGAc > 1:2897214/1‑41 (MQ=255) gCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCggg > 1:3981773/1‑47 (MQ=255) gCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGc > 1:856705/1‑65 (MQ=255) cTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATa < 1:4011876/70‑1 (MQ=255) tCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATa > 1:3507984/1‑69 (MQ=255) tCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATa > 1:524350/1‑69 (MQ=255) cAGACCAACCCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCa > 1:3454120/1‑59 (MQ=255) cAGACCAAACCGAATAACCTCATGACGATCTGCGGACAg > 1:3946739/1‑39 (MQ=255) cAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATa < 1:380857/67‑1 (MQ=255) cAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAgg > 1:2995590/1‑71 (MQ=255) aGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAg < 1:2769988/69‑1 (MQ=255) aGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAg < 1:1525584/69‑1 (MQ=255) gACCAAACCGAATAACCTCATGACGATCTGCGGACAGCggg < 1:3880923/41‑1 (MQ=255) gACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTc > 1:904916/1‑55 (MQ=255) gACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTc > 1:2084564/1‑55 (MQ=255) gACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCa > 1:2653523/1‑57 (MQ=255) gACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCa > 1:1290153/1‑57 (MQ=255) gACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTa > 1:3114407/1‑71 (MQ=255) gACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTa > 1:1952879/1‑71 (MQ=255) gACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTa > 1:821253/1‑71 (MQ=255) ccAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGt > 1:1626608/1‑68 (MQ=255) ccAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATc < 1:2697189/71‑1 (MQ=255) cAAACCGAATAACCTCATGACGGTCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATcc < 1:2733132/71‑1 (MQ=255) cAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATc > 1:1503416/1‑70 (MQ=255) aaaCCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGt < 1:2204244/66‑1 (MQ=255) | TAATTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCC > minE/165091‑165229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |