| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,496 | A→G | 8.8% | F459F (TTT→TTC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,496 | 0 | A | G | 8.8% | 257.5 / 14.0 | 91 | F459F (TTT→TTC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (40/43); new base G (3/5); total (43/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCC > minE/311426‑311566 | gTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa < 1:798959/71‑1 (MQ=255) ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa > 1:4080458/1‑68 (MQ=255) ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag < 1:2055908/71‑1 (MQ=255) ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag < 1:4008394/71‑1 (MQ=255) aCAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaag > 1:1475278/1‑71 (MQ=255) gcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGaag < 1:3311858/63‑1 (MQ=12) aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaaa < 1:217482/59‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:1240749/1‑70 (MQ=255) ttGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGaag < 1:2683586/59‑1 (MQ=9) tGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaaa < 1:4143381/57‑1 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:884535/1‑71 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:3313949/1‑71 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:3593586/1‑71 (MQ=255) cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:1075745/1‑70 (MQ=255) cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:1052156/1‑70 (MQ=255) cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:2634234/71‑1 (MQ=255) cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:3604915/71‑1 (MQ=255) cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:445949/71‑1 (MQ=255) cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:2696760/71‑1 (MQ=255) gAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGaaga < 1:612709/55‑1 (MQ=12) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa > 1:1214635/1‑70 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa < 1:3294276/70‑1 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa > 1:2967883/1‑70 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt < 1:456851/71‑1 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt < 1:726494/71‑1 (MQ=255) aaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa < 1:3808073/49‑1 (MQ=255) aCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:928449/66‑1 (MQ=255) cAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAcc < 1:603014/71‑1 (MQ=255) cAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAcc < 1:2620980/71‑1 (MQ=255) tGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc < 1:1802132/71‑1 (MQ=255) gTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa < 1:73839/71‑1 (MQ=255) aTAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaaa < 1:1344794/41‑1 (MQ=255) agagAACTGACGATAGATAGCACCAGTAGGACCGCCAAAGAAGGCCAt > 1:2305752/1‑48 (MQ=255) gagaACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCAc > 1:2879698/1‑71 (MQ=9) gagaACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:2737850/1‑41 (MQ=255) gagaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa > 1:2858637/1‑52 (MQ=255) gagaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt < 1:3472467/57‑1 (MQ=255) gagaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt < 1:54172/57‑1 (MQ=255) gagaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt < 1:69656/57‑1 (MQ=255) gagaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt < 1:2858723/57‑1 (MQ=255) agaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:497705/53‑1 (MQ=255) agaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc < 1:2908535/71‑1 (MQ=255) gaACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGa > 1:3653763/1‑63 (MQ=9) aaCTGACGATAGATAGCACCAGTAGAACCGCCaaa > 1:1190044/1‑35 (MQ=255) aaCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc < 1:1774125/60‑1 (MQ=255) cTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATcg > 1:3074943/1‑71 (MQ=255) cTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa > 1:2611265/1‑51 (MQ=255) gACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc > 1:2217854/1‑70 (MQ=255) gACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc > 1:2164353/1‑70 (MQ=255) gcGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGa < 1:57355/70‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa > 1:1042407/1‑48 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:135907/69‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:3675328/69‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:2122659/69‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:2649993/69‑1 (MQ=255) cGATAAATTGCCCCAGTAGAACCGCCGGAGAATGCCATCGGAATAAATAcc < 1:2682735/51‑1 (MQ=255) atagatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg < 1:2630602/57‑1 (MQ=255) atagatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg < 1:566775/57‑1 (MQ=255) tagatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:510594/42‑1 (MQ=255) agatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc > 1:203348/1‑70 (MQ=255) gatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:2847626/40‑1 (MQ=255) atagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:440967/62‑1 (MQ=255) atagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:136807/62‑1 (MQ=255) agCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGAc > 1:1004187/1‑69 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:2320443/1‑61 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCag < 1:304715/71‑1 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCag < 1:1857996/71‑1 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCag < 1:3208573/71‑1 (MQ=255) cACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc < 1:1104317/44‑1 (MQ=255) aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc < 1:1521534/52‑1 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc > 1:3144201/1‑49 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc > 1:81596/1‑48 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:3139594/1‑71 (MQ=255) gTAGAACCGCCAAAGAAGGCCATCGGTACGAATAc > 1:821858/1‑35 (MQ=255) tAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:3356690/52‑1 (MQ=255) aGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg > 1:2280982/1‑42 (MQ=255) aGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg > 1:3104664/1‑42 (MQ=255) aGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGccc > 1:3841881/1‑70 (MQ=255) aGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:1238850/1‑71 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:3635217/50‑1 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:2131698/1‑52 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa > 1:551963/1‑61 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGccc > 1:3752865/1‑69 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:3295307/1‑70 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:3172011/1‑70 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:1065092/1‑70 (MQ=255) aaCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:2121909/71‑1 (MQ=255) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:907978/70‑1 (MQ=255) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:2506293/70‑1 (MQ=255) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:307462/70‑1 (MQ=255) ccGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:3389224/1‑64 (MQ=255) ccGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:2925843/1‑64 (MQ=255) ccGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:3394083/1‑64 (MQ=255) cGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc < 1:2007500/71‑1 (MQ=255) gCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc > 1:2684275/1‑39 (MQ=255) gCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGaa > 1:1976328/1‑68 (MQ=255) cAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt > 1:859337/1‑40 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:917748/1‑62 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:1683033/64‑1 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTccc < 1:1776318/71‑1 (MQ=255) | GTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCC > minE/311426‑311566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |