| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,562 | A→G | 9.8% | L346L (TTG→CTG) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,562 | 0 | A | G | 9.8% | 280.4 / 19.0 | 102 | L346L (TTG→CTG) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base A (52/40); new base G (8/2); total (60/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
GCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGC > minE/2595494‑2595630 | gCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcg < 1:3030406/71‑1 (MQ=255) ccAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg > 1:3492090/1‑70 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:250142/1‑71 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:1982458/1‑71 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc < 1:1189527/71‑1 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCa < 1:3039604/71‑1 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCa < 1:3010525/71‑1 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc > 1:3792116/1‑71 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc > 1:1472393/1‑71 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc < 1:2885897/70‑1 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc > 1:3042308/1‑66 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa < 1:2423512/71‑1 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg < 1:1339796/70‑1 (MQ=255) ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa > 1:843990/1‑71 (MQ=255) ggCATAAACCATCGCTTCTTTGATAACGCCAGAAGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa > 1:2676502/1‑71 (MQ=255) cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc < 1:920206/64‑1 (MQ=255) cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc < 1:668832/71‑1 (MQ=255) cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc < 1:2578237/71‑1 (MQ=255) cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc < 1:2447130/71‑1 (MQ=255) cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc < 1:2098857/71‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc < 1:190327/62‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc < 1:3149685/69‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt > 1:3616433/1‑71 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:12138/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa > 1:357494/1‑62 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:81717/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:3892744/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:3014557/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg < 1:310282/71‑1 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:3349748/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGAACAACGCGCCAACCAGTACCGTCg < 1:2289317/71‑1 (MQ=255) cATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCa < 1:2187272/71‑1 (MQ=255) aTCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCCGCat < 1:1576686/71‑1 (MQ=255) tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa > 1:2786869/1‑57 (MQ=255) tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata < 1:3855249/71‑1 (MQ=255) cGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat > 1:1477518/1‑71 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa > 1:2183550/1‑44 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt > 1:2203847/1‑71 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt > 1:3478741/1‑71 (MQ=255) cttcttTGATAACGCCAGATGCCGTGCTTCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:1520909/71‑1 (MQ=255) cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:1238127/71‑1 (MQ=255) ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa > 1:1295209/1‑70 (MQ=255) tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat < 1:1604755/70‑1 (MQ=255) aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata < 1:2527304/69‑1 (MQ=255) aaCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt > 1:2260972/1‑62 (MQ=255) aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatcac < 1:3165685/68‑4 (MQ=255) aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatcac < 1:2500424/68‑4 (MQ=255) cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa < 1:477469/71‑1 (MQ=255) gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCat < 1:424490/54‑1 (MQ=255) ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:1733267/71‑1 (MQ=255) cAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:57482/70‑1 (MQ=255) gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag < 1:273273/71‑1 (MQ=255) gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag < 1:902455/71‑1 (MQ=255) gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag < 1:897302/71‑1 (MQ=255) tcgCCGTACTCCCGTCAATCGACAGCGCACCAATCATCACCGTCGGCATATTGTTAATAACTGAtgaga > 1:1648825/3‑69 (MQ=255) aTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:2344369/67‑1 (MQ=255) tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:2814061/1‑44 (MQ=255) tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa < 1:3914127/71‑1 (MQ=255) tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGCCATATTGTTCATAATAGAAGAGAgg > 1:3063473/1‑70 (MQ=255) cgCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGAtgagaga > 1:301409/2‑69 (MQ=255) gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAgg > 1:2012762/1‑69 (MQ=255) gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa < 1:2279910/70‑1 (MQ=255) gTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata > 1:1538798/1‑45 (MQ=255) tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata > 1:1029990/1‑56 (MQ=255) ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:3794525/1‑51 (MQ=255) ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt > 1:1287844/1‑71 (MQ=255) ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt < 1:2412370/71‑1 (MQ=255) aTCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt < 1:3900776/66‑1 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGGATATTGTTCata > 1:2555150/1‑45 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:393022/1‑45 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag < 1:2739145/56‑1 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa > 1:3784368/1‑67 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAgg < 1:2350959/69‑1 (MQ=255) tCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTc > 1:3653462/1‑42 (MQ=19) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa > 1:4045008/1‑58 (MQ=255) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa > 1:2348929/1‑57 (MQ=255) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa > 1:2060411/1‑57 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa > 1:3300282/1‑44 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa > 1:1483563/1‑41 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa > 1:740986/1‑65 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATAtt > 1:1908294/1‑36 (MQ=16) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGAtgagagaa > 1:698983/1‑54 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGAtgagagaa > 1:2820743/1‑54 (MQ=255) tcgACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCg > 1:4096321/3‑71 (MQ=255) tGGATAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAgg < 1:581612/57‑1 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg > 1:1849194/1‑71 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAGGAATCCg > 1:4035344/1‑71 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg > 1:3341765/1‑57 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt > 1:515988/1‑59 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:1366220/1‑70 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGt > 1:403082/1‑71 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGt < 1:3599026/71‑1 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGt > 1:904402/1‑71 (MQ=255) cAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata > 1:2970419/1‑40 (MQ=255) cAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata > 1:1952911/1‑40 (MQ=255) cAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata > 1:1415244/1‑40 (MQ=255) aaCGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc > 1:3508107/1‑70 (MQ=255) aCGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:925498/1‑35 (MQ=255) aCGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag < 1:3312691/44‑1 (MQ=255) aCGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag < 1:958/44‑1 (MQ=255) | GCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGC > minE/2595494‑2595630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |