Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,571 C→T 10.2% V343I (GTT→ATT)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,5710CT10.2% 298.4 / 17.4 98V343I (GTT→ATT) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (51/37);  new base T (7/3);  total (58/40)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

ACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCG  >  minE/2595501‑2595632
                                                                      |                                                             
acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                               >  1:3792116/1‑71 (MQ=255)
acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                               >  1:1472393/1‑71 (MQ=255)
 caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                               <  1:2885897/70‑1 (MQ=255)
  aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                             <  1:2423512/71‑1 (MQ=255)
    tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                                            <  1:1339796/70‑1 (MQ=255)
     ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa                                                          >  1:843990/1‑71 (MQ=255)
     ggCATAAACCATCGCTTCTTTGATAACGCCAGAAGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa                                                          >  1:2676502/1‑71 (MQ=255)
       cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                               <  1:920206/64‑1 (MQ=255)
       cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                                        <  1:2098857/71‑1 (MQ=255)
       cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                                        <  1:2578237/71‑1 (MQ=255)
       cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                                        <  1:2447130/71‑1 (MQ=255)
       cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                                        <  1:668832/71‑1 (MQ=255)
         tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                               <  1:190327/62‑1 (MQ=255)
         tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                                        <  1:3149685/69‑1 (MQ=255)
         tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                                      >  1:2218321/1‑71 (MQ=255)
         tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                                      >  1:3616433/1‑71 (MQ=255)
          aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                     >  1:12138/1‑71 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                             >  1:357494/1‑62 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                    <  1:310282/71‑1 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                    >  1:3892744/1‑71 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                    >  1:3678812/1‑71 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                    >  1:81717/1‑71 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                    >  1:3349748/1‑71 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                    >  1:3014557/1‑71 (MQ=255)
              cATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCa                                                 <  1:2187272/71‑1 (MQ=255)
               aTCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCCGCat                                                <  1:1576686/71‑1 (MQ=255)
                tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                             >  1:2786869/1‑57 (MQ=255)
                tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata                                               <  1:3855249/71‑1 (MQ=255)
                 cGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat                                              >  1:1477518/1‑71 (MQ=255)
                  gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                             >  1:2203847/1‑71 (MQ=255)
                  gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                             >  1:3478741/1‑71 (MQ=255)
                   cttcttTGATAACGCCAGATGCCGTGCTTCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                            <  1:1520909/71‑1 (MQ=255)
                   cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                            <  1:1238127/71‑1 (MQ=255)
                        ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa                                        >  1:1295209/1‑70 (MQ=255)
                         tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                                       <  1:1604755/70‑1 (MQ=255)
                           aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                      <  1:2527304/69‑1 (MQ=255)
                             aaCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                           >  1:2260972/1‑62 (MQ=255)
                              aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatcac                                    <  1:3165685/68‑4 (MQ=255)
                              aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatcac                                    <  1:2500424/68‑4 (MQ=255)
                               cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                <  1:477469/71‑1 (MQ=255)
                                gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCat                                                <  1:424490/54‑1 (MQ=255)
                                 ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                              <  1:1733267/71‑1 (MQ=255)
                                  cAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                              <  1:57482/70‑1 (MQ=255)
                                    gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag                           <  1:902455/71‑1 (MQ=255)
                                    gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag                           <  1:273273/71‑1 (MQ=255)
                                    gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag                           <  1:897302/71‑1 (MQ=255)
                                     tcgCCGTACTCCCGTCAATCGACAGCGCACCAATCATCACCGTCGGCATATTGTTAATAACTGAtgaga                            >  1:1648825/3‑69 (MQ=255)
                                     aTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                              <  1:2344369/67‑1 (MQ=255)
                                      tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                    >  1:2814061/1‑44 (MQ=255)
                                      tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga                            >  1:265722/1‑68 (MQ=255)
                                      tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa                         <  1:3914127/71‑1 (MQ=255)
                                      tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGCCATATTGTTCATAATAGAAGAGAgg                          >  1:3063473/1‑70 (MQ=255)
                                      cgCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGAtgagaga                          >  1:301409/2‑69 (MQ=255)
                                       gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAgg                          >  1:2012762/1‑69 (MQ=255)
                                       gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa                         <  1:2279910/70‑1 (MQ=255)
                                          gTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata                                               >  1:1538798/1‑45 (MQ=255)
                                           tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata                                   >  1:1029990/1‑56 (MQ=255)
                                             ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                      >  1:3794525/1‑51 (MQ=255)
                                             ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt                  <  1:2412370/71‑1 (MQ=255)
                                             ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt                  >  1:1287844/1‑71 (MQ=255)
                                                  aTCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt                  <  1:3900776/66‑1 (MQ=255)
                                                   tCAATGGACAACGCGCCAACCAGTACCGTCGGGATATTGTTCata                                      >  1:2555150/1‑45 (MQ=255)
                                                   tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                      >  1:393022/1‑45 (MQ=255)
                                                   tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag                           <  1:2739145/56‑1 (MQ=255)
                                                   tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa                >  1:3784368/1‑67 (MQ=255)
                                                   tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAgg              <  1:2350959/69‑1 (MQ=255)
                                                   tCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTc                                         >  1:3653462/1‑42 (MQ=19)
                                                    cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa                        >  1:4045008/1‑58 (MQ=255)
                                                    cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa                        >  1:1597676/1‑58 (MQ=255)
                                                    cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa                         >  1:2348929/1‑57 (MQ=255)
                                                    cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa                         >  1:2060411/1‑57 (MQ=255)
                                                     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                     >  1:3300282/1‑44 (MQ=255)
                                                     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa                                        >  1:1483563/1‑41 (MQ=255)
                                                     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa                >  1:740986/1‑65 (MQ=255)
                                                     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc          >  1:3072922/1‑71 (MQ=255)
                                                     aaTCGACAGCGCACCAATCAGCACCGTCGGCATAtt                                             >  1:1908294/1‑36 (MQ=16)
                                                     aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGAtgagagaa                         >  1:2820743/1‑54 (MQ=255)
                                                     aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGAtgagagaa                         >  1:698983/1‑54 (MQ=255)
                                                       tcgACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCg        >  1:4096321/3‑71 (MQ=255)
                                                       tGGATAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAgg                      <  1:581612/57‑1 (MQ=255)
                                                       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg        >  1:1849194/1‑71 (MQ=255)
                                                       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAGGAATCCg        >  1:4035344/1‑71 (MQ=255)
                                                         gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg                    >  1:3341765/1‑57 (MQ=255)
                                                         gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt                  >  1:515988/1‑59 (MQ=255)
                                                         gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg       >  1:1366220/1‑70 (MQ=255)
                                                         gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGt      >  1:403082/1‑71 (MQ=255)
                                                         gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGt      >  1:904402/1‑71 (MQ=255)
                                                           cAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata                                   >  1:2970419/1‑40 (MQ=255)
                                                           cAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata                                   >  1:1415244/1‑40 (MQ=255)
                                                           cAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata                                   >  1:1952911/1‑40 (MQ=255)
                                                            aaCGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc    >  1:3508107/1‑70 (MQ=255)
                                                             gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg  <  1:1548561/70‑1 (MQ=255)
                                                             aCGCGCCAACCAGTACCGTCGGCATATTGTTCata                                      >  1:925498/1‑35 (MQ=255)
                                                             aCGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag                             <  1:958/44‑1 (MQ=255)
                                                             aCGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag                             <  1:3312691/44‑1 (MQ=255)
                                                              cgcgCCAACCAGTTCCGTCGGCATATTGTTCataata                                   >  1:61593/1‑37 (MQ=255)
                                                              cgcgCCAACCAGTACCGTCGGCATATTGTTCataata                                   >  1:797370/1‑37 (MQ=255)
                                                                  ccAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAgg              <  1:1364406/54‑1 (MQ=255)
                                                                   cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg               >  1:1403787/1‑52 (MQ=255)
                                                                     aCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAgg                          <  1:1030686/39‑1 (MQ=255)
                                                                      |                                                             
ACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCG  >  minE/2595501‑2595632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: