| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,575 | T→C | 10.5% | V341V (GTA→GTG) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,575 | 0 | T | C | 10.5% | 260.8 / 19.0 | 95 | V341V (GTA→GTG) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base T (50/35); new base C (7/3); total (57/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGC > minE/2595506‑2595643 | ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa > 1:843990/1‑71 (MQ=255)ggCATAAACCATCGCTTCTTTGATAACGCCAGAAGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa > 1:2676502/1‑71 (MQ=255) cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc < 1:668832/71‑1 (MQ=255) cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc < 1:2578237/71‑1 (MQ=255) cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc < 1:2447130/71‑1 (MQ=255) cATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc < 1:2098857/71‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc < 1:3149685/69‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt > 1:3616433/1‑71 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt > 1:2218321/1‑71 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:12138/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:81717/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg < 1:310282/71‑1 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:3678812/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:3349748/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:3892744/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:3014557/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGAACAACGCGCCAACCAGTACCGTCg < 1:2289317/71‑1 (MQ=255) cATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCa < 1:2187272/71‑1 (MQ=255) aTCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCCGCat < 1:1576686/71‑1 (MQ=255) tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata < 1:3855249/71‑1 (MQ=255) cGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat > 1:1477518/1‑71 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt > 1:3478741/1‑71 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt > 1:2203847/1‑71 (MQ=255) cttcttTGATAACGCCAGATGCCGTGCTTCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:1520909/71‑1 (MQ=255) cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:1238127/71‑1 (MQ=255) ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa > 1:1295209/1‑70 (MQ=255) tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat < 1:1604755/70‑1 (MQ=255) aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa < 1:976142/70‑1 (MQ=255) aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata < 1:2527304/69‑1 (MQ=255) aaCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt > 1:2260972/1‑62 (MQ=255) aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatcac < 1:3165685/68‑4 (MQ=255) aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatcac < 1:2500424/68‑4 (MQ=255) cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa < 1:477469/71‑1 (MQ=255) gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCat < 1:424490/54‑1 (MQ=255) ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:1733267/71‑1 (MQ=255) cAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:57482/70‑1 (MQ=255) gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag < 1:897302/71‑1 (MQ=255) gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag < 1:902455/71‑1 (MQ=255) gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag < 1:273273/71‑1 (MQ=255) tcgCCGTACTCCCGTCAATCGACAGCGCACCAATCATCACCGTCGGCATATTGTTAATAACTGAtgaga > 1:1648825/3‑69 (MQ=255) aTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:2344369/67‑1 (MQ=255) tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:2814061/1‑44 (MQ=255) tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga > 1:265722/1‑68 (MQ=255) tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa < 1:3914127/71‑1 (MQ=255) tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGCCATATTGTTCATAATAGAAGAGAgg > 1:3063473/1‑70 (MQ=255) cgCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGAtgagaga > 1:301409/2‑69 (MQ=255) gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAgg > 1:2012762/1‑69 (MQ=255) gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa < 1:2279910/70‑1 (MQ=255) gTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata > 1:1538798/1‑45 (MQ=255) tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata > 1:1029990/1‑56 (MQ=255) ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:3794525/1‑51 (MQ=255) ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt < 1:2412370/71‑1 (MQ=255) ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt > 1:1287844/1‑71 (MQ=255) aTCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt < 1:3900776/66‑1 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGGATATTGTTCata > 1:2555150/1‑45 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:393022/1‑45 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag < 1:2739145/56‑1 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa > 1:3784368/1‑67 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAgg < 1:2350959/69‑1 (MQ=255) tCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTc > 1:3653462/1‑42 (MQ=19) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa > 1:4045008/1‑58 (MQ=255) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa > 1:1597676/1‑58 (MQ=255) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa > 1:2348929/1‑57 (MQ=255) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa > 1:2060411/1‑57 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa > 1:3300282/1‑44 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa > 1:1483563/1‑41 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa > 1:740986/1‑65 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc > 1:3072922/1‑71 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATAtt > 1:1908294/1‑36 (MQ=16) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGAtgagagaa > 1:698983/1‑54 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGAtgagagaa > 1:2820743/1‑54 (MQ=255) tcgACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCg > 1:4096321/3‑71 (MQ=255) tGGATAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAgg < 1:581612/57‑1 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg > 1:1849194/1‑71 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAGGAATCCg > 1:4035344/1‑71 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg > 1:3341765/1‑57 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt > 1:515988/1‑59 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:1366220/1‑70 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGt > 1:403082/1‑71 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGt < 1:3599026/71‑1 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGt > 1:904402/1‑71 (MQ=255) cAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata > 1:2970419/1‑40 (MQ=255) cAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata > 1:1952911/1‑40 (MQ=255) cAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata > 1:1415244/1‑40 (MQ=255) aaCGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc > 1:3508107/1‑70 (MQ=255) gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:1548561/70‑1 (MQ=255) aCGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:925498/1‑35 (MQ=255) aCGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag < 1:3312691/44‑1 (MQ=255) aCGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag < 1:958/44‑1 (MQ=255) cgcgCCAACCAGTTCCGTCGGCATATTGTTCataata > 1:61593/1‑37 (MQ=255) cgcgCCAACCAGTACCGTCGGCATATTGTTCataata > 1:797370/1‑37 (MQ=255) ccAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAgg < 1:1364406/54‑1 (MQ=255) cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg > 1:1403787/1‑52 (MQ=255) aCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAgg < 1:1030686/39‑1 (MQ=255) aGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGc > 1:3102491/1‑71 (MQ=255) | GGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGC > minE/2595506‑2595643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |