| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,737 | G→C | 14.8% | L287L (CTC→CTG) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,737 | 0 | G | C | 14.8% | 236.8 / 26.7 | 94 | L287L (CTC→CTG) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (39/41); new base C (6/8); total (45/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
GTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTT > minE/2595668‑2595807 | gTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcga < 1:3101453/71‑1 (MQ=255) ttGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgag < 1:637127/71‑1 (MQ=255) tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc > 1:1962964/1‑71 (MQ=255) tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc > 1:1370070/1‑71 (MQ=255) gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc > 1:795533/1‑70 (MQ=255) gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc < 1:2370377/70‑1 (MQ=255) gTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag < 1:3264086/71‑1 (MQ=255) tACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag > 1:1309749/1‑70 (MQ=255) cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag > 1:3096479/1‑68 (MQ=255) cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag > 1:3686725/1‑68 (MQ=255) cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag > 1:3842745/1‑68 (MQ=255) gCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga < 1:3723524/71‑1 (MQ=255) gCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga < 1:3607875/71‑1 (MQ=255) ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt > 1:3782293/1‑71 (MQ=255) ccAGAAAGATAATCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt < 1:3180948/71‑1 (MQ=255) cAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga < 1:2127303/69‑1 (MQ=255) aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa > 1:2427970/1‑71 (MQ=255) gAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa > 1:1155118/1‑70 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc > 1:510221/1‑70 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:1492527/71‑1 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:2321938/71‑1 (MQ=255) atatTCCGTTAATCCGGCATTGCTCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt < 1:4053749/63‑1 (MQ=255) atatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGAGagaaga > 1:3770257/1‑62 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:2306123/70‑1 (MQ=255) tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag < 1:856557/57‑1 (MQ=255) tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:740359/1‑69 (MQ=255) gTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg > 1:3618205/1‑71 (MQ=255) ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:628440/1‑65 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt > 1:572633/1‑59 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt > 1:3752041/1‑59 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCAccag < 1:677764/69‑3 (MQ=255) ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAta < 1:377836/45‑2 (MQ=255) ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAta < 1:1532692/45‑2 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa < 1:1272313/41‑1 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt < 1:2983377/44‑1 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:2026522/48‑1 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt < 1:180973/52‑1 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:1668365/54‑1 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCgcgc < 1:70289/69‑1 (MQ=255) tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:3425185/1‑70 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGagaaga > 1:3223350/1‑41 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt > 1:3388879/1‑42 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATg > 1:1397900/1‑43 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa > 1:3033549/1‑44 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa > 1:2406516/1‑54 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa > 1:2359650/1‑54 (MQ=255) cAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:325150/71‑1 (MQ=255) cAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:2898541/71‑1 (MQ=255) cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt < 1:3579096/71‑1 (MQ=255) cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt < 1:2720411/71‑1 (MQ=255) aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACttt < 1:2918027/71‑1 (MQ=255) aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt < 1:3526430/70‑1 (MQ=255) ggCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCgcgc < 1:3944700/62‑1 (MQ=255) gCCATAAACCACCAGATACATGCCGAGCGAGAAGAt < 1:824554/36‑1 (MQ=255) gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa > 1:37375/1‑65 (MQ=255) cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc > 1:161659/1‑71 (MQ=255) cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc < 1:474058/71‑1 (MQ=255) cATAAACAACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg > 1:358062/1‑48 (MQ=255) aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg < 1:351432/71‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:1701324/37‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:2568257/36‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:3172504/36‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:2303278/36‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt > 1:4033837/1‑40 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt > 1:2356609/1‑40 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:1820424/49‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg < 1:299629/48‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg < 1:807049/46‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg > 1:4079911/1‑70 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg < 1:1543030/70‑1 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTg > 1:3003348/1‑40 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTg > 1:2998659/1‑40 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:1056650/1‑41 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa > 1:1050198/1‑71 (MQ=255) aaaCCACCAGATACATGCCCAGCGAGAAGATGACAATCTGc > 1:1192800/1‑41 (MQ=255) aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCa > 1:2956606/1‑49 (MQ=255) accaccAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt < 1:3981654/71‑1 (MQ=255) ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg > 1:4042943/1‑43 (MQ=255) ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa < 1:3118844/71‑1 (MQ=255) caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa > 1:1221211/1‑71 (MQ=255) caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg < 1:873797/71‑1 (MQ=255) caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg > 1:3218807/1‑71 (MQ=255) gATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCa > 1:479754/1‑71 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:19379/38‑1 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCa < 1:1026382/40‑1 (MQ=255) tACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa > 1:1446015/1‑64 (MQ=255) tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAAt < 1:3560186/70‑1 (MQ=255) aCATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa > 1:1143159/1‑71 (MQ=255) cATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg < 1:1546797/65‑1 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:2411812/1‑45 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:1187780/1‑45 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt > 1:3468886/1‑50 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt > 1:2056368/1‑50 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg < 1:3724475/71‑1 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg > 1:1150191/1‑71 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg > 1:3715574/1‑71 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc < 1:1239249/70‑1 (MQ=255) cgagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc > 1:1707907/1‑69 (MQ=255) gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGt > 1:3120323/1‑51 (MQ=255) gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:2354371/1‑71 (MQ=255) | GTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTT > minE/2595668‑2595807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |