Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,595,752 | A→G | 16.7% | I282I (ATT→ATC) | arsB ← | arsenite/antimonite transporter |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,595,752 | 0 | A | G | 16.7% | 183.8 / 29.9 | 78 | I282I (ATT→ATC) | arsB | arsenite/antimonite transporter |
Reads supporting (aligned to +/- strand): ref base A (39/26); new base G (6/7); total (45/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.76e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01 |
AAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAA > minE/2595682‑2595820 | aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:909375/71‑1 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:1492527/71‑1 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:2321938/71‑1 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:2306123/70‑1 (MQ=255) tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:740359/1‑69 (MQ=255) gTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg > 1:3618205/1‑71 (MQ=255) ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:628440/1‑65 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt > 1:3752041/1‑59 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt > 1:572633/1‑59 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCAccag < 1:677764/69‑3 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:2026522/48‑1 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt < 1:180973/52‑1 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:1668365/54‑1 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCgcgc < 1:70289/69‑1 (MQ=255) tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:3425185/1‑70 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa > 1:2406516/1‑54 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa > 1:2359650/1‑54 (MQ=255) cAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:2898541/71‑1 (MQ=255) cAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:3630922/71‑1 (MQ=255) cAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:325150/71‑1 (MQ=255) cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt < 1:3579096/71‑1 (MQ=255) cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt < 1:2720411/71‑1 (MQ=255) aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACttt < 1:2918027/71‑1 (MQ=255) aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt < 1:3526430/70‑1 (MQ=255) ggCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCgcgc < 1:3944700/62‑1 (MQ=255) gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa > 1:37375/1‑65 (MQ=255) cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc < 1:474058/71‑1 (MQ=255) cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc > 1:161659/1‑71 (MQ=255) cATAAACAACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg > 1:358062/1‑48 (MQ=255) aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg < 1:351432/71‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:611245/37‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:1701324/37‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:2568257/36‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:3172504/36‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:2303278/36‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt > 1:4033837/1‑40 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt > 1:2356609/1‑40 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:1820424/49‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg < 1:299629/48‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg < 1:807049/46‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg < 1:1543030/70‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg > 1:4079911/1‑70 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTg > 1:3003348/1‑40 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTg > 1:2998659/1‑40 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:307417/1‑41 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:1056650/1‑41 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa > 1:1050198/1‑71 (MQ=255) aaaCCACCAGATACATGCCCAGCGAGAAGATGACAATCTGc > 1:1192800/1‑41 (MQ=255) aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCa > 1:2956606/1‑49 (MQ=255) accaccAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt < 1:3981654/71‑1 (MQ=255) ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg > 1:4042943/1‑43 (MQ=255) ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa < 1:3118844/71‑1 (MQ=255) caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa > 1:1221211/1‑71 (MQ=255) caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg > 1:3218807/1‑71 (MQ=255) caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg < 1:873797/71‑1 (MQ=255) aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa > 1:2292813/1‑63 (MQ=255) gATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCa > 1:479754/1‑71 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:19379/38‑1 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCa < 1:1026382/40‑1 (MQ=255) tACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa > 1:1446015/1‑64 (MQ=255) tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAAt < 1:3560186/70‑1 (MQ=255) tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATg < 1:1272004/71‑1 (MQ=255) aCATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa > 1:1143159/1‑71 (MQ=255) cATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg < 1:1546797/65‑1 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:2411812/1‑45 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:1187780/1‑45 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt > 1:2056368/1‑50 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt > 1:3468886/1‑50 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg > 1:3715574/1‑71 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg < 1:3724475/71‑1 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg > 1:1150191/1‑71 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc < 1:1239249/70‑1 (MQ=255) cgagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc > 1:1707907/1‑69 (MQ=255) gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGt > 1:3120323/1‑51 (MQ=255) gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:2354371/1‑71 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcg < 1:542549/66‑1 (MQ=255) gcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTa > 1:575450/1‑44 (MQ=255) gcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt > 1:4146121/1‑52 (MQ=255) tgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGttg < 1:1255131/51‑2 (MQ=255) cgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc > 1:310340/1‑46 (MQ=255) gagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGc > 1:2720152/1‑71 (MQ=255) gaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg < 1:2428047/41‑1 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg < 1:3595693/66‑1 (MQ=255) gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac > 1:3410138/1‑66 (MQ=255) gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa > 1:2972399/1‑70 (MQ=255) aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa > 1:3746971/1‑71 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa > 1:3127947/1‑70 (MQ=255) | AAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAA > minE/2595682‑2595820 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |