Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,595,761 | G→T | 17.9% | P279P (CCC→CCA) | arsB ← | arsenite/antimonite transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,595,761 | 0 | G | T | 17.9% | 218.6 / 19.3 | 72 | P279P (CCC→CCA) | arsB | arsenite/antimonite transporter |
Reads supporting (aligned to +/- strand): ref base G (31/28); new base T (6/7); total (37/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.64e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGC > minE/2595693‑2595830 | gTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg > 1:3618205/1‑71 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCAccag < 1:677764/69‑3 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCgcgc < 1:70289/69‑1 (MQ=255) tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:3425185/1‑70 (MQ=255) cAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:2898541/71‑1 (MQ=255) cAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:325150/71‑1 (MQ=255) cAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:3630922/71‑1 (MQ=255) cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt < 1:2720411/71‑1 (MQ=255) cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt < 1:3579096/71‑1 (MQ=255) aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACttt < 1:2918027/71‑1 (MQ=255) aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt < 1:3526430/70‑1 (MQ=255) ggCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCgcgc < 1:3944700/62‑1 (MQ=255) gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa > 1:37375/1‑65 (MQ=255) cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc > 1:161659/1‑71 (MQ=255) cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc < 1:474058/71‑1 (MQ=255) cATAAACAACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg > 1:358062/1‑48 (MQ=255) aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg < 1:351432/71‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:1820424/49‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg < 1:299629/48‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg < 1:807049/46‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg < 1:1543030/70‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg > 1:4079911/1‑70 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa > 1:1050198/1‑71 (MQ=255) aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCa > 1:2956606/1‑49 (MQ=255) accaccAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt < 1:3981654/71‑1 (MQ=255) ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg > 1:4042943/1‑43 (MQ=255) ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa < 1:3118844/71‑1 (MQ=255) caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa > 1:1221211/1‑71 (MQ=255) caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg > 1:3218807/1‑71 (MQ=255) caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg < 1:873797/71‑1 (MQ=255) aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa > 1:2292813/1‑63 (MQ=255) gATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCa > 1:479754/1‑71 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:19379/38‑1 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCa < 1:1026382/40‑1 (MQ=255) tACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa > 1:1446015/1‑64 (MQ=255) tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAAt < 1:3560186/70‑1 (MQ=255) tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATg < 1:1272004/71‑1 (MQ=255) aCATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa > 1:1143159/1‑71 (MQ=255) cATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg < 1:1546797/65‑1 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:2411812/1‑45 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:1187780/1‑45 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt > 1:2056368/1‑50 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt > 1:3468886/1‑50 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg > 1:3715574/1‑71 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg > 1:1150191/1‑71 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg < 1:3724475/71‑1 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc < 1:1239249/70‑1 (MQ=255) cgagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc > 1:1707907/1‑69 (MQ=255) gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGt > 1:3120323/1‑51 (MQ=255) gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:2354371/1‑71 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcg < 1:542549/66‑1 (MQ=255) gcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTa > 1:575450/1‑44 (MQ=255) gcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt > 1:4146121/1‑52 (MQ=255) tgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGttg < 1:1255131/51‑2 (MQ=255) cgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc > 1:310340/1‑46 (MQ=255) gagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGc > 1:2720152/1‑71 (MQ=255) gaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg < 1:2428047/41‑1 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg < 1:3595693/66‑1 (MQ=255) gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac > 1:3410138/1‑66 (MQ=255) gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa > 1:2972399/1‑70 (MQ=255) aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa > 1:3746971/1‑71 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa > 1:3127947/1‑70 (MQ=255) aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa < 1:2015137/40‑1 (MQ=255) aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataa < 1:649158/71‑1 (MQ=255) tCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataa > 1:2263615/1‑70 (MQ=255) cTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa > 1:424177/1‑37 (MQ=255) tGCCAGGGGGCACCGCTCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat > 1:1346485/1‑71 (MQ=255) tGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAt > 1:2794700/1‑43 (MQ=255) tGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataa > 1:339359/1‑68 (MQ=255) tGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat > 1:3684755/1‑71 (MQ=255) tGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat > 1:1679962/1‑71 (MQ=255) gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga > 1:63436/1‑57 (MQ=255) ccAGTGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg < 1:2647464/55‑1 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg < 1:1266369/55‑1 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg < 1:349721/55‑1 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat < 1:1589338/67‑1 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAataata < 1:4052833/68‑1 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa < 1:959771/71‑1 (MQ=255) cAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAg < 1:936051/71‑1 (MQ=255) gggggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgc < 1:70213/70‑1 (MQ=255) | GTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGC > minE/2595693‑2595830 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |