| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,596,130 | G→A | 9.6% | V156V (GTC→GTT) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,596,130 | 0 | G | A | 9.6% | 221.5 / 11.2 | 73 | V156V (GTC→GTT) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (34/32); new base A (4/3); total (38/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTT > minE/2596062‑2596200 | aTCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGAc < 1:1835696/71‑1 (MQ=255)aTCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACAAGGTTGgaga > 1:441133/1‑70 (MQ=255) tCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGAc < 1:3843012/70‑1 (MQ=255) tCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGAc > 1:2976417/1‑70 (MQ=255) tCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGAc > 1:1716669/1‑70 (MQ=255) tCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGAc > 1:286727/1‑70 (MQ=255) aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa < 1:3558223/70‑1 (MQ=255) aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa > 1:321323/1‑70 (MQ=255) aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa > 1:2293903/1‑70 (MQ=255) aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa > 1:2273499/1‑70 (MQ=255) aGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGgaga < 1:1119755/63‑1 (MQ=255) gTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCa < 1:2965322/71‑1 (MQ=255) tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg < 1:1864799/68‑1 (MQ=255) ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGc > 1:481599/1‑71 (MQ=255) ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGc > 1:1105007/1‑71 (MQ=255) ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGc > 1:425572/1‑71 (MQ=255) ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGc > 1:2903001/1‑71 (MQ=255) ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGc > 1:3602550/1‑71 (MQ=255) tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATaa < 1:3453981/61‑1 (MQ=255) cgcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTg > 1:3162516/1‑71 (MQ=255) gcgAATGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTgg < 1:80226/71‑1 (MQ=255) cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg > 1:3307063/4‑71 (MQ=255) cGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTCGAGACAATAAGCGGCAGGCTGGc < 1:2813100/64‑1 (MQ=255) aGGCCAATGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:3031067/71‑1 (MQ=255) aGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:1867257/71‑1 (MQ=255) aGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:660342/71‑1 (MQ=255) gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa > 1:3831951/1‑44 (MQ=255) ccAAAGAAATCAGCGGAAACGATATTCTCCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt > 1:2133069/1‑71 (MQ=255) ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:3334549/71‑1 (MQ=255) ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:3797684/71‑1 (MQ=255) cAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCgg > 1:3883718/1‑66 (MQ=255) aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGa < 1:3242990/71‑1 (MQ=255) aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGa < 1:2125149/71‑1 (MQ=255) aagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATaaa > 1:750249/1‑71 (MQ=255) aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg < 1:920293/58‑1 (MQ=255) aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa < 1:3136089/71‑1 (MQ=255) aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt < 1:2531797/71‑1 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAg > 1:515603/1‑42 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGt < 1:3708694/57‑1 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc > 1:1136039/1‑71 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc > 1:2964234/1‑71 (MQ=255) aaaTCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc < 1:2731646/70‑1 (MQ=255) aGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg < 1:2174770/50‑1 (MQ=255) gCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg > 1:3634524/1‑49 (MQ=255) gCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgccgc > 1:2606000/1‑71 (MQ=255) cGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAg > 1:761210/1‑40 (MQ=255) cGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAg > 1:1870491/1‑40 (MQ=255) ggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCa > 1:437794/1‑71 (MQ=255) ggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCa > 1:2265911/1‑71 (MQ=255) ggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCa < 1:2142609/71‑1 (MQ=255) gAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt < 1:1563665/71‑1 (MQ=255) aCGATATTCACCAGGTTGGAGACAATAAGCGGCAg < 1:580537/35‑1 (MQ=255) gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa < 1:3354604/71‑3 (MQ=255) gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa < 1:3350762/71‑3 (MQ=255) atatTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCg < 1:2202926/45‑1 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:3358153/44‑1 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt < 1:3587156/62‑1 (MQ=255) tCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:2080108/1‑70 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:806710/1‑69 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:591246/1‑69 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:3254166/1‑69 (MQ=255) aCCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAg > 1:338330/1‑71 (MQ=255) cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg < 1:1680970/71‑1 (MQ=255) cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGACAGc > 1:256378/1‑70 (MQ=255) cAGGTTGGAGACAATAAGCCGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGc > 1:3229683/1‑70 (MQ=255) aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGt < 1:411312/71‑1 (MQ=255) aGGTTAGAAACAATGCGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc > 1:958970/1‑65 (MQ=255) aGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc > 1:1915426/1‑65 (MQ=255) aGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgt < 1:128093/71‑1 (MQ=255) ttGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg < 1:3983386/67‑1 (MQ=255) ttGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGt < 1:2078208/71‑1 (MQ=255) tGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg > 1:3775205/1‑71 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc > 1:32683/1‑41 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg > 1:3551316/1‑70 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg < 1:2050154/70‑1 (MQ=255) gagaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGcc < 1:2140427/71‑1 (MQ=255) gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt < 1:309246/71‑1 (MQ=255) | ATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTT > minE/2596062‑2596200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |