| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,596,151 | G→C | 10.9% | T149T (ACC→ACG) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,596,151 | 0 | G | C | 10.9% | 177.7 / 11.1 | 64 | T149T (ACC→ACG) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (24/33); new base C (4/3); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
AGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGC > minE/2596089‑2596216 | aGGCCAATGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:3031067/71‑1 (MQ=255)aGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:660342/71‑1 (MQ=255)aGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:1867257/71‑1 (MQ=255) ccAAAGAAATCAGCGGAAACGATATTCTCCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt > 1:2133069/1‑71 (MQ=255) ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:3334549/71‑1 (MQ=255) ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:3797684/71‑1 (MQ=255) cAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCgg > 1:3883718/1‑66 (MQ=255) aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGa < 1:3242990/71‑1 (MQ=255) aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGa < 1:2125149/71‑1 (MQ=255) aagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATaaa > 1:750249/1‑71 (MQ=255) aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg < 1:920293/58‑1 (MQ=255) aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa < 1:3136089/71‑1 (MQ=255) aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt < 1:2531797/71‑1 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGt < 1:3708694/57‑1 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc > 1:1136039/1‑71 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc > 1:2964234/1‑71 (MQ=255) aaaTCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc < 1:2731646/70‑1 (MQ=255) aGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg < 1:2174770/50‑1 (MQ=255) gCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg > 1:3634524/1‑49 (MQ=255) gCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgccgc > 1:2606000/1‑71 (MQ=255) ggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCa > 1:437794/1‑71 (MQ=255) ggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCa > 1:2265911/1‑71 (MQ=255) ggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCa < 1:2142609/71‑1 (MQ=255) gAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt < 1:1563665/71‑1 (MQ=255) gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa < 1:3354604/71‑3 (MQ=255) gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa < 1:3350762/71‑3 (MQ=255) atatTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCg < 1:2202926/45‑1 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:3358153/44‑1 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt < 1:3587156/62‑1 (MQ=255) tCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:2080108/1‑70 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:591246/1‑69 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:806710/1‑69 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:3254166/1‑69 (MQ=255) aCCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAg > 1:338330/1‑71 (MQ=255) cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg < 1:1680970/71‑1 (MQ=255) cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGACAGc > 1:256378/1‑70 (MQ=255) cAGGTTGGAGACAATAAGCCGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGc > 1:3229683/1‑70 (MQ=255) aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGt < 1:411312/71‑1 (MQ=255) aGGTTAGAAACAATGCGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc > 1:958970/1‑65 (MQ=255) aGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc > 1:1915426/1‑65 (MQ=255) aGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgt < 1:128093/71‑1 (MQ=255) ttGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg < 1:3983386/67‑1 (MQ=255) ttGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGt < 1:2078208/71‑1 (MQ=255) tGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg > 1:3775205/1‑71 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc > 1:32683/1‑41 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg < 1:2050154/70‑1 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg > 1:3551316/1‑70 (MQ=255) gagaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGcc < 1:2140427/71‑1 (MQ=255) gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt < 1:309246/71‑1 (MQ=255) aCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGccctg > 1:3790992/1‑68 (MQ=255) cAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTa < 1:3885421/71‑1 (MQ=255) cAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTa > 1:1714446/1‑71 (MQ=255) tAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt > 1:2121772/1‑70 (MQ=255) tAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt < 1:2807375/70‑1 (MQ=255) tAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTg < 1:3601908/71‑1 (MQ=255) tAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTg < 1:334970/71‑1 (MQ=255) aaGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCATCGTAGTGCCTTTACTg > 1:2710512/1‑70 (MQ=255) aGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTa > 1:1080377/1‑57 (MQ=255) aGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGaa > 1:2727881/1‑71 (MQ=255) gCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAAc > 1:2215050/1‑71 (MQ=255) gCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAAc > 1:1842847/1‑71 (MQ=255) cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:2701181/71‑1 (MQ=255) cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:3277368/71‑1 (MQ=255) cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:92884/71‑1 (MQ=255) gCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAg < 1:1033214/71‑1 (MQ=255) cTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGc < 1:470088/71‑1 (MQ=255) cTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGc < 1:3423796/71‑1 (MQ=255) ggTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAg < 1:2002415/65‑1 (MQ=255) | AGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGC > minE/2596089‑2596216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |