| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,596,169 | G→T | 6.7% | A143A (GCC→GCA) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,596,169 | 0 | G | T | 6.7% | 299.7 / 4.4 | 89 | A143A (GCC→GCA) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (42/41); new base T (3/3); total (45/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01 | |||||||||||
GCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATC > minE/2596104‑2596238 | gCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgccgc > 1:2606000/1‑71 (MQ=255) ggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCa < 1:2142609/71‑1 (MQ=255) ggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCa > 1:2265911/1‑71 (MQ=255) ggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCa > 1:437794/1‑71 (MQ=255) gAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt < 1:1563665/71‑1 (MQ=255) gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa < 1:3354604/71‑3 (MQ=255) gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa < 1:3350762/71‑3 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt < 1:3587156/62‑1 (MQ=255) tCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:2080108/1‑70 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:3254166/1‑69 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:591246/1‑69 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:806710/1‑69 (MQ=255) aCCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAg > 1:338330/1‑71 (MQ=255) cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg < 1:1680970/71‑1 (MQ=255) cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGACAGc > 1:256378/1‑70 (MQ=255) cAGGTTGGAGACAATAAGCCGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGc > 1:3229683/1‑70 (MQ=255) aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGt < 1:411312/71‑1 (MQ=255) aGGTTAGAAACAATGCGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc > 1:958970/1‑65 (MQ=255) aGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc > 1:1915426/1‑65 (MQ=255) aGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgt < 1:128093/71‑1 (MQ=255) ttGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg < 1:3983386/67‑1 (MQ=255) ttGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGt < 1:2078208/71‑1 (MQ=255) tGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg > 1:3775205/1‑71 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg > 1:3551316/1‑70 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg < 1:2050154/70‑1 (MQ=255) gagaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGcc < 1:2140427/71‑1 (MQ=255) gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt < 1:309246/71‑1 (MQ=255) aCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGccctg > 1:3790992/1‑68 (MQ=255) cAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTa > 1:1714446/1‑71 (MQ=255) cAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTa < 1:3885421/71‑1 (MQ=255) tAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt > 1:2121772/1‑70 (MQ=255) tAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt < 1:2807375/70‑1 (MQ=255) tAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTg < 1:3601908/71‑1 (MQ=255) tAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTg < 1:334970/71‑1 (MQ=255) aaGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCATCGTAGTGCCTTTACTg > 1:2710512/1‑70 (MQ=255) aGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTa > 1:1080377/1‑57 (MQ=255) aGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGaa > 1:2727881/1‑71 (MQ=255) gCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAAc > 1:2215050/1‑71 (MQ=255) gCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAAc > 1:1842847/1‑71 (MQ=255) cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:2701181/71‑1 (MQ=255) cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:3277368/71‑1 (MQ=255) cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:92884/71‑1 (MQ=255) gCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAg < 1:1033214/71‑1 (MQ=255) cTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGc < 1:3423796/71‑1 (MQ=255) cTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGc < 1:470088/71‑1 (MQ=255) ggTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAg < 1:2002415/65‑1 (MQ=255) gTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCcagcag < 1:1193840/71‑1 (MQ=255) gTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCcagcag < 1:2371791/71‑1 (MQ=255) tATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTg > 1:2696969/1‑53 (MQ=255) tATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCagcagc < 1:3416614/71‑1 (MQ=255) aTCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTAc > 1:821160/1‑50 (MQ=255) aTCGGCAATGAATCCGACCGCCATCACGAACGCCa > 1:413182/1‑35 (MQ=255) tCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg < 1:2963690/37‑1 (MQ=255) ggCAATGAATCCGGCCGCCATCACGAACGCCAGCGt > 1:2521008/1‑36 (MQ=255) ggCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAg > 1:1383372/1‑38 (MQ=255) ggCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCGTTTa > 1:2888205/1‑46 (MQ=255) ggCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATgg > 1:3601550/1‑71 (MQ=255) ggCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATgg > 1:81407/1‑71 (MQ=255) ggCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATgg > 1:1178081/1‑71 (MQ=255) ggCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATgg > 1:1195497/1‑71 (MQ=255) ggCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATg > 1:901798/1‑70 (MQ=255) gCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGc < 1:305547/71‑1 (MQ=255) gCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGc < 1:3502706/71‑1 (MQ=255) aaTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt < 1:4118040/41‑1 (MQ=255) aaTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGa > 1:2992834/1‑71 (MQ=255) aTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt < 1:970924/40‑1 (MQ=255) aTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt < 1:1090316/40‑1 (MQ=255) aTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGAt < 1:3310172/71‑1 (MQ=255) aTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGAt < 1:3874024/71‑1 (MQ=255) tGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATg < 1:2740918/71‑1 (MQ=255) tGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATg < 1:1123222/71‑1 (MQ=255) tGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATg < 1:753872/71‑1 (MQ=255) tGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATg < 1:381507/71‑1 (MQ=255) gAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTa > 1:630507/1‑40 (MQ=255) gAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTAc > 1:1799294/1‑41 (MQ=255) gAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGa > 1:2622781/1‑71 (MQ=255) aaTCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTa > 1:891372/1‑39 (MQ=255) aaTCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt > 1:3225410/1‑41 (MQ=255) aaTCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt > 1:245192/1‑41 (MQ=255) aaTCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGa > 1:177270/1‑67 (MQ=255) aaTCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGAt < 1:2752507/68‑1 (MQ=255) aaTCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGa > 1:1024029/1‑70 (MQ=255) aaTCCGGCCGCCATCACGAACGCCAGAGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGAt < 1:3357043/68‑1 (MQ=255) aTCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGAt < 1:44709/67‑1 (MQ=255) aTCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACa < 1:2354083/71‑1 (MQ=255) ccGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAAt > 1:3158369/1‑71 (MQ=255) cGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATc > 1:2541705/1‑71 (MQ=255) cGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATc < 1:3738856/71‑1 (MQ=255) cGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATc > 1:3355953/1‑71 (MQ=255) | GCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATC > minE/2596104‑2596238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |