| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,620,159 | C→T | 7.8% | A194T (GCA→ACA) | zntA ← | zinc, cobalt and lead efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,620,159 | 0 | C | T | 7.8% | 283.0 / 10.4 | 90 | A194T (GCA→ACA) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base C (33/50); new base T (5/2); total (38/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
AGTCGTTCACCAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCGTAATGCC > minE/2620092‑2620229 | aGTCGTTCACCAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGc < 1:2949311/68‑1 (MQ=255)aGTCGTTCACCAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCa > 1:3212510/1‑69 (MQ=255)aGTCGTTCACCAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCa > 1:1948920/1‑69 (MQ=255) tCGTTCACCAATTAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCaa < 1:2393611/71‑1 (MQ=255) tCGTTCACCAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCAc > 1:2967426/1‑68 (MQ=255) tCGTTCACCAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCAc > 1:1638634/1‑68 (MQ=255) gTTCACCAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGTACCaa > 1:3316744/1‑69 (MQ=255) gTTCACCAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCaa > 1:2369017/1‑69 (MQ=255) gTTCACAAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCaa > 1:201812/1‑69 (MQ=255) cACCAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCg > 1:2583330/1‑71 (MQ=255) aCCAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTCCGCCAATAAACAGTGCACCAATAGCg < 1:2886677/70‑1 (MQ=255) aaTCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGTACCAATAGCGGCTa > 1:1023268/1‑71 (MQ=255) tCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCAcc < 1:1734129/58‑1 (MQ=255) tCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACg < 1:177487/71‑1 (MQ=255) cAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCa > 1:3052940/1‑55 (MQ=255) cAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTa > 1:37869/1‑68 (MQ=255) aaaaaCAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGc < 1:3534269/53‑1 (MQ=255) aaaaaCAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGc < 1:2399430/70‑1 (MQ=255) aaaaaCAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGc > 1:3307465/1‑70 (MQ=255) aaaaaCAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGc < 1:1828731/70‑1 (MQ=255) aaaaaCAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCt < 1:280401/71‑1 (MQ=255) aaaaaCAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCt < 1:2897446/71‑1 (MQ=255) aaCAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCAt > 1:2181295/1‑71 (MQ=255) aCAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCAtt < 1:1649864/71‑1 (MQ=255) gcagcaACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAg < 1:3717744/71‑1 (MQ=255) cagcaACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAgg > 1:107298/1‑71 (MQ=255) gcaACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGc < 1:3845221/52‑1 (MQ=255) caACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCAcc < 1:3733244/45‑1 (MQ=255) aaCACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCTGCTACGCTCATTAAGGTTTc < 1:101935/71‑1 (MQ=255) aaCACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTc < 1:3754322/71‑1 (MQ=255) aaCACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTc < 1:975994/71‑1 (MQ=255) aaCACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTc < 1:2603142/71‑1 (MQ=255) cacCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATa < 1:1364081/46‑1 (MQ=255) cacCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCaa < 1:2771954/71‑1 (MQ=255) ccATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCaa > 1:772096/1‑42 (MQ=255) ccATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCAt < 1:2063498/58‑1 (MQ=255) tCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCaa > 1:3253434/1‑39 (MQ=255) cGCAGCTTCAGCCGTTGCGCCAATAAACAGTGTACCAATAGCGGCTACGCTCATTAAGGTTTc > 1:919201/1‑63 (MQ=255) gCAGCTTCAGCCGTTGCGCCAATAAACAGTGTACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCg > 1:3969774/1‑69 (MQ=255) gCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGtt < 1:2113965/60‑1 (MQ=255) gCAGCTTCAGCCGTTGCCCCAATAAACAGTGTACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCg > 1:2460210/1‑69 (MQ=255) aGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGTCTACGCTCATTAAGGTTTCAATGGCGAAGt < 1:3803608/71‑1 (MQ=255) aGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGc > 1:179590/1‑66 (MQ=255) aGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGt < 1:1308666/71‑1 (MQ=255) aGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGt < 1:2241279/71‑1 (MQ=255) gCTTCAGCCGTTGCGCCAATAAACAGTGTACCAATAGCGGCTACGCTCATTAAGGTTTCa < 1:2838741/60‑1 (MQ=255) cTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGt < 1:3052074/69‑1 (MQ=255) ttCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGt < 1:827236/54‑1 (MQ=255) tCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCaa > 1:2650426/1‑58 (MQ=255) tCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCa > 1:93150/1‑57 (MQ=255) gCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGcc < 1:1874567/71‑1 (MQ=255) cGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCaa > 1:1513472/1‑53 (MQ=255) gTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGa < 1:1957528/71‑1 (MQ=255) ttGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTc < 1:3855958/49‑1 (MQ=255) ttGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTc < 1:3848412/49‑1 (MQ=255) ttGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGAt < 1:784251/71‑1 (MQ=255) ccAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTc < 1:2923278/44‑1 (MQ=255) ccAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGAt < 1:1336855/71‑1 (MQ=255) cAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCaa > 1:458448/1‑45 (MQ=255) cAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCaa > 1:334615/1‑45 (MQ=255) cAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCaa > 1:3135896/1‑45 (MQ=255) cAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCaa > 1:979722/1‑45 (MQ=255) cAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGAt > 1:1743788/1‑70 (MQ=255) cAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGAt > 1:1049958/1‑70 (MQ=255) cAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGAt > 1:1890544/1‑70 (MQ=255) aaTAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCg < 1:515382/61‑1 (MQ=255) aaTAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATc > 1:2792395/1‑70 (MQ=255) aTAAACAGTGTACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTa < 1:1661552/53‑1 (MQ=255) aTAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCaa > 1:3647462/1‑71 (MQ=255) tAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGt < 1:2699195/37‑1 (MQ=255) tAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCg < 1:3007761/47‑1 (MQ=255) tAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGAt > 1:2946351/1‑67 (MQ=255) aaaCAGCGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGa > 1:830397/1‑65 (MQ=255) aaCAGTGCACCAATAGCGGCTACGCTCATTAAGGtt < 1:2930400/36‑1 (MQ=255) aaCAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTc < 1:2837455/38‑1 (MQ=255) aaCAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGaa < 1:13795/47‑1 (MQ=255) aaCAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAAc < 1:762568/69‑1 (MQ=255) aaCAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCg < 1:3260647/71‑1 (MQ=255) aaCAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCg > 1:1542861/1‑71 (MQ=255) aCAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGaa < 1:428274/46‑1 (MQ=255) aCAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCGt < 1:2192444/71‑1 (MQ=255) aCAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCGt < 1:1673181/71‑1 (MQ=255) aCAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCGt < 1:1217184/71‑1 (MQ=255) aCAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCGt < 1:2673820/71‑1 (MQ=255) cAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATgg > 1:674052/1‑41 (MQ=255) cAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCg > 1:2000989/1‑43 (MQ=255) aGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTg < 1:4099098/60‑1 (MQ=255) aGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCGTaa < 1:3481106/71‑1 (MQ=255) gTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTg < 1:886282/59‑1 (MQ=255) gTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCGTAAt < 1:3485398/71‑1 (MQ=255) gCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCGt < 1:3326795/66‑1 (MQ=255) gCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCGTAATGc > 1:2247398/1‑71 (MQ=255) cACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGaa > 1:1734181/1‑40 (MQ=255) cACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCGTAATGcc > 1:2018267/1‑71 (MQ=255) | AGTCGTTCACCAATCAAAAACAGCAGCAACACCATCGCAGCTTCAGCCGTTGCGCCAATAAACAGTGCACCAATAGCGGCTACGCTCATTAAGGTTTCAATGGCGAAGTAGCTGCCGGATTTGATCAACCGTAATGCC > minE/2620092‑2620229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |