| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,623,821 | G→A | 8.6% | A292A (GCG→GCA) | ftsY → | fused Signal Recognition Particle (SRP) receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,623,821 | 0 | G | A | 8.6% | 217.1 / 8.3 | 70 | A292A (GCG→GCA) | ftsY | fused Signal Recognition Particle (SRP) receptor |
| Reads supporting (aligned to +/- strand): ref base G (39/25); new base A (3/3); total (42/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGC > minE/2623756‑2623886 | ctgAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGtt > 1:3567320/1‑71 (MQ=255) gAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTtg > 1:2157909/1‑71 (MQ=255) aagaagAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTTAAGGCAAAGCGCCGttt < 1:1075418/68‑1 (MQ=255) aagaagAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCACCGTTtgtg < 1:1405413/71‑1 (MQ=255) agaagaGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTtg > 1:1678123/1‑68 (MQ=255) agaagaGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGa > 1:3926541/1‑71 (MQ=255) agaagaGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGa > 1:117104/1‑71 (MQ=255) agaagaGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGa < 1:45744/71‑1 (MQ=255) aagaGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTtg > 1:3626892/1‑66 (MQ=255) aagaGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATc > 1:202242/1‑71 (MQ=255) agagATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATcc > 1:1361989/1‑71 (MQ=255) gagaTGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCt > 1:2644139/1‑71 (MQ=255) gaTGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGa < 1:1686852/71‑1 (MQ=255) aTGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGAt < 1:2480320/71‑1 (MQ=255) aTGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGAt < 1:3400927/71‑1 (MQ=255) gggCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGAtg > 1:3241878/1‑70 (MQ=255) ggCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTTAtggt < 1:2782800/71‑1 (MQ=255) ggCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGAtggt < 1:3142293/71‑1 (MQ=255) gCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTtgtg > 1:3370383/1‑59 (MQ=255) gaTTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCg > 1:2565754/1‑71 (MQ=255) gaTTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCACCGTTTGTGATCCTGAtg > 1:1420529/1‑64 (MQ=255) aTTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGt > 1:1886931/1‑71 (MQ=255) ttCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTc < 1:3574073/71‑1 (MQ=255) cTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATc > 1:2028806/1‑54 (MQ=255) cTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCaa > 1:230052/1‑71 (MQ=255) gCGAAAGTCGATGAGCCTCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACgg < 1:806026/71‑1 (MQ=255) gCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACgg > 1:2181137/1‑71 (MQ=255) gCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACgg > 1:1279079/1‑71 (MQ=255) gCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACgg > 1:4044785/1‑71 (MQ=255) gCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACgg > 1:3219552/1‑71 (MQ=255) gAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGgtg < 1:2656290/71‑1 (MQ=255) aaaGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTtgt < 1:4119764/44‑1 (MQ=255) aaaGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTtgt < 1:1754351/44‑1 (MQ=255) aGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGa > 1:1615238/1‑50 (MQ=255) tCGATGAGCCGCTGAATGTTGAAGGCAAAGCACCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTggg > 1:1413920/1‑70 (MQ=255) gATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGgt > 1:1512944/1‑63 (MQ=255) aTGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGgt < 1:1988669/62‑1 (MQ=255) aTGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTaa < 1:1673415/70‑1 (MQ=255) aTGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTaa > 1:2658877/1‑70 (MQ=255) gAGCCTCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAAc > 1:3065261/1‑71 (MQ=255) ccGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATcc < 1:505333/37‑1 (MQ=255) gCTGAATGTTGAAGTCAAAGCACCGTTTGTGATCCTGATGGTGGGCGTCAACGgtg < 1:4026502/56‑1 (MQ=255) gCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCacga > 1:1500215/1‑71 (MQ=255) gCTGAATGTTGAAGGCAAAGCACCGTTTGTGATCCTGATGGTGGGCGTCAACGgtg < 1:2759386/56‑1 (MQ=255) cTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTaaaa > 1:2916052/1‑64 (MQ=255) cTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTaa < 1:2637882/62‑1 (MQ=255) gAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGAtgg < 1:304333/38‑1 (MQ=255) gAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCAcgacga > 1:2055416/1‑71 (MQ=255) gAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCAcgacga > 1:68042/1‑71 (MQ=255) aaTGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCAcgacga < 1:2587058/70‑1 (MQ=255) aaTGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGGCAACGGTGTGGGTAAAACCAcgacga < 1:701953/70‑1 (MQ=255) aTGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACg > 1:1483938/1‑48 (MQ=255) tGTTGAAGGCAAAGCGCCGTTTGTGATCCTGAtgt < 1:199255/35‑2 (MQ=255) tGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGgt > 1:2165388/1‑49 (MQ=255) tGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTaa < 1:3517909/57‑1 (MQ=255) tGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTg > 1:2475607/1‑71 (MQ=255) tGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTg > 1:2736743/1‑71 (MQ=255) tGTTGAAGGCAAAGCACCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTaaaa > 1:3211991/1‑59 (MQ=255) ttGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCaa > 1:2466006/1‑43 (MQ=255) ttGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTgg > 1:2465374/1‑70 (MQ=255) ttGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTgg > 1:242259/1‑70 (MQ=255) ctGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTaaaa > 1:1623273/2‑57 (MQ=255) tGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGt < 1:1025800/70‑1 (MQ=255) gAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTa < 1:1173799/70‑1 (MQ=255) ggCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCacg < 1:3067373/57‑1 (MQ=255) cAAAGCGCCGTTTGTTATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCacga > 1:3676393/1‑56 (MQ=255) cAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACgg > 1:2685781/1‑39 (MQ=255) cAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCacgac > 1:3886908/1‑57 (MQ=255) cAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTgg > 1:4004999/1‑71 (MQ=255) aaaGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGgc < 1:1991526/71‑1 (MQ=255) gcgcCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCacga > 1:2815959/1‑52 (MQ=255) | CTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGC > minE/2623756‑2623886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |