| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,677,365 | T→A | 5.8% | M189L (ATG→TTG) | yhgA ← | predicted transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,677,365 | 0 | T | A | 5.8% | 290.8 / 6.6 | 100 | M189L (ATG→TTG) | yhgA | predicted transposase |
| Reads supporting (aligned to +/- strand): ref base T (46/48); new base A (2/4); total (48/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
AGCCCTTTGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCA > minE/2677295‑2677435 | aGCCCTTTGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCAt < 1:2373082/71‑1 (MQ=255)aGCCCTTTGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCAt < 1:16741/71‑1 (MQ=255) ccTTTGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAg > 1:1102464/1‑71 (MQ=255) tttGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCAt < 1:1565761/66‑1 (MQ=255) tttGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGt > 1:2199140/1‑71 (MQ=255) ttGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTc > 1:3654813/1‑71 (MQ=255) tGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCa > 1:3145825/1‑66 (MQ=255) tGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTcg > 1:2625266/1‑71 (MQ=255) gATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTcg < 1:346026/70‑1 (MQ=255) tttGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCAt < 1:86885/61‑1 (MQ=255) tttGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGt < 1:221221/71‑1 (MQ=255) ttGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGtt > 1:136073/1‑71 (MQ=255) tgtctgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCa > 1:3739294/1‑61 (MQ=255) tgtctgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGtt < 1:4053693/70‑1 (MQ=255) tgtctgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGtt < 1:4058677/70‑1 (MQ=255) tctgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTcg < 1:3558525/64‑1 (MQ=255) tctgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTcg < 1:309144/64‑1 (MQ=255) tctgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTg < 1:1585615/69‑1 (MQ=255) tcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACg < 1:1741979/68‑1 (MQ=255) tcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGaa > 1:2686998/1‑70 (MQ=255) tcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGaa > 1:3120466/1‑70 (MQ=255) aTTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAata < 1:414950/70‑1 (MQ=255) aTTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAata > 1:2305166/1‑70 (MQ=255) tAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGt > 1:428572/1‑71 (MQ=255) tAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGt > 1:2309589/1‑71 (MQ=255) aGCGTATGCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGt < 1:3899308/70‑1 (MQ=255) gCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttt < 1:3523145/71‑1 (MQ=255) tATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTg < 1:1693229/71‑1 (MQ=255) tATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTg < 1:1478516/71‑1 (MQ=255) tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGt > 1:2540253/1‑47 (MQ=255) tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa < 1:2394082/71‑1 (MQ=255) tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa > 1:2423498/1‑71 (MQ=255) tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa > 1:2467873/1‑71 (MQ=255) tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa < 1:2320473/71‑1 (MQ=255) cACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTc > 1:3821274/1‑46 (MQ=255) cACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAata > 1:2310815/1‑59 (MQ=255) cACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAt > 1:4106154/1‑70 (MQ=255) cACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAt > 1:2443369/1‑70 (MQ=255) cACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAt > 1:2448139/1‑70 (MQ=255) ctactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAg < 1:2930299/41‑1 (MQ=255) ctactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt < 1:2446042/69‑1 (MQ=255) actTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAAc > 1:2880092/1‑70 (MQ=255) ctTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa > 1:2647398/1‑64 (MQ=255) ctTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAAc < 1:3700832/69‑1 (MQ=255) ctTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTc > 1:1250402/1‑71 (MQ=255) ttAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAg < 1:1580466/37‑1 (MQ=255) ttAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTa < 1:906971/66‑1 (MQ=255) aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGtt > 1:386124/1‑55 (MQ=255) aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc < 1:3523774/69‑1 (MQ=255) ataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTcgcg < 1:2580624/40‑1 (MQ=255) ataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag < 1:2213232/70‑1 (MQ=255) aGCAGGCCATTTGCTCTACCAGGCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcagcag < 1:2688793/70‑1 (MQ=255) aGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGtt < 1:3361935/51‑1 (MQ=255) ggCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt < 1:1618980/55‑1 (MQ=255) ggCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa > 1:1229529/1‑71 (MQ=255) gCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttt > 1:638692/1‑47 (MQ=255) cATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAat > 1:1445616/1‑39 (MQ=255) aTTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt < 1:2643060/51‑1 (MQ=255) ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGt > 1:3659000/1‑40 (MQ=255) ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTaa > 1:3955401/1‑51 (MQ=255) ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc > 1:2854845/1‑63 (MQ=255) ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATa > 1:1678606/1‑67 (MQ=255) ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc > 1:2458756/1‑71 (MQ=255) tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGtttt > 1:485469/1‑42 (MQ=255) tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACt > 1:2636880/1‑52 (MQ=255) tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc < 1:785570/70‑1 (MQ=255) tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc < 1:780550/70‑1 (MQ=255) tGCTCTACAAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg > 1:2220854/1‑71 (MQ=255) gCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc < 1:1143270/69‑1 (MQ=255) gCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg < 1:1103915/70‑1 (MQ=255) tctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcagc < 1:2475029/54‑1 (MQ=255) tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTg < 1:3492251/39‑1 (MQ=255) tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTg < 1:1320179/39‑1 (MQ=255) tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt < 1:2524417/43‑1 (MQ=255) ccAGCCCCATCAGGTCGCGTTGACGAATATGtttt > 1:44879/1‑35 (MQ=255) ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa > 1:1236983/1‑39 (MQ=255) ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCcgcg > 1:2626706/1‑54 (MQ=255) ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg < 1:3055109/70‑1 (MQ=255) cAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa < 1:1888385/71‑1 (MQ=255) aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag > 1:2314943/1‑47 (MQ=255) aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt < 1:2273773/71‑1 (MQ=255) aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt > 1:213696/1‑71 (MQ=255) aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt < 1:3037099/71‑1 (MQ=255) gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt < 1:580160/38‑1 (MQ=255) gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt > 1:686605/1‑70 (MQ=255) atcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAt < 1:2748717/68‑1 (MQ=255) tcccAACAGATCGCGCTGACGAATATGTTTCTGAAtt < 1:1801600/36‑1 (MQ=16) tcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCca < 1:130344/43‑1 (MQ=21) tcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCca < 1:831525/43‑1 (MQ=21) ccccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc > 1:823735/1‑56 (MQ=255) cccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAt < 1:2768629/71‑1 (MQ=255) ccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCcaac > 1:1769764/1‑42 (MQ=25) ccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCcaa > 1:3777964/1‑42 (MQ=25) aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc < 1:2636595/47‑1 (MQ=255) aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg > 1:718769/1‑56 (MQ=255) aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGa < 1:3594158/67‑1 (MQ=255) tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCgg > 1:1112838/1‑56 (MQ=255) tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg < 1:405496/55‑1 (MQ=255) tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg < 1:2665079/65‑1 (MQ=255) tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca > 1:3925326/1‑71 (MQ=255) | AGCCCTTTGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCA > minE/2677295‑2677435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |