BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,390	T→C	9.7%	Q180Q (CAA→CAG)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,390	0	T	C	9.7%	349.6 / 23.9	124	Q180Q (CAA→CAG)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base T (56/56); new base C (3/9); total (59/65)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 135 total aligned reads displayed.

TATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCA  >  minE/2677322‑2677459

tATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTg                                                                     <  1:1693229/71‑1 (MQ=255)

  tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa                                                                   <  1:2320473/71‑1 (MQ=255)

  tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa                                                                   >  1:2423498/1‑71 (MQ=255)

  tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa                                                                   >  1:2467873/1‑71 (MQ=255)

    cACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAt                                                                  >  1:477640/1‑70 (MQ=255)

    cACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAt                                                                  >  1:4106154/1‑70 (MQ=255)

    cACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAt                                                                  >  1:2448139/1‑70 (MQ=255)

      ctactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                 <  1:2446042/69‑1 (MQ=255)

        actTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAAc                                                              >  1:2880092/1‑70 (MQ=255)

         ctTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTc                                                            >  1:1250402/1‑71 (MQ=255)

            aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                                           <  1:3523774/69‑1 (MQ=255)

                aGCAGGCCATTTGCTCTACCAGGCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcagcag                                                      <  1:2688793/70‑1 (MQ=255)

                  cAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                   >  1:3481060/1‑71 (MQ=255)

                    ggCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                 <  1:1618980/55‑1 (MQ=255)

                        aTTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                 <  1:2643060/51‑1 (MQ=255)

                        aTTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                 <  1:1549073/51‑1 (MQ=255)

                          ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATa                                               >  1:1678606/1‑67 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGtttt                                                                       >  1:485469/1‑42 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACt                                                             >  1:2636880/1‑52 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                           <  1:785570/70‑1 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                           <  1:780550/70‑1 (MQ=255)

                           tGCTCTACAAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                          >  1:2220854/1‑71 (MQ=255)

                            gCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                           <  1:1143270/69‑1 (MQ=255)

                            gCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                          <  1:1103915/70‑1 (MQ=255)

                              tctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcagc                                                        <  1:2475029/54‑1 (MQ=255)

                                tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTg                                                                     <  1:3492251/39‑1 (MQ=255)

                                tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                 <  1:2524417/43‑1 (MQ=255)

                                  ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa                                                                   >  1:1236983/1‑39 (MQ=255)

                                  ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCcgcg                                                    >  1:2626706/1‑54 (MQ=255)

                                  ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                    <  1:23658/70‑1 (MQ=255)

                                  ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                    <  1:3055109/70‑1 (MQ=255)

                                   cAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                  <  1:1888385/71‑1 (MQ=255)

                                    aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                 <  1:2273773/71‑1 (MQ=255)

                                    aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                 >  1:213696/1‑71 (MQ=255)

                                    aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                 <  1:3037099/71‑1 (MQ=255)

                                     gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                 <  1:580160/38‑1 (MQ=255)

                                     gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                 >  1:686605/1‑70 (MQ=255)

                                     atcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAt                                 <  1:2748717/68‑1 (MQ=255)

                                      tcccAACAGATCGCGCTGACGAATATGTTTCTGAAtt                                                                 <  1:1801600/36‑1 (MQ=16)

                                      tcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCca                                                          <  1:130344/43‑1 (MQ=21)

                                      tcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCca                                                          <  1:831525/43‑1 (MQ=21)

                                        ccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCcaac                                                        >  1:1769764/1‑42 (MQ=25)

                                        ccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCcaa                                                         >  1:3777964/1‑42 (MQ=25)

                                          aacAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAAtctc                           <  1:867482/69‑1 (MQ=255)

                                          aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                          >  1:718769/1‑56 (MQ=255)

                                          aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGa                               <  1:3594158/67‑1 (MQ=255)

                                           tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                          <  1:405496/55‑1 (MQ=255)

                                           tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                <  1:2665079/65‑1 (MQ=255)

                                           tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca                          >  1:3925326/1‑71 (MQ=255)

                                             aGGTCGCGTTGATGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtt                             <  1:1152073/66‑1 (MQ=255)

                                             aGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                                           >  1:3872801/1‑36 (MQ=255)

                                             aGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                                           >  1:1940963/1‑36 (MQ=255)

                                             aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                 >  1:3829174/1‑46 (MQ=255)

                                             aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATa                                               >  1:2398820/1‑48 (MQ=255)

                                             aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                            >  1:2140804/1‑51 (MQ=255)

                                             aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                 <  1:2743089/62‑1 (MQ=255)

                                             aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                         >  1:2628654/1‑70 (MQ=255)

                                             aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                         >  1:1449557/1‑70 (MQ=255)

                                              ggTCGCGTTGACGAATATGTTTTTGAATTAACTCcagcag                                                      >  1:3195019/1‑40 (MQ=255)

                                               gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                   <  1:1615508/42‑1 (MQ=255)

                                               atCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAta                                <  1:1673469/60‑2 (MQ=255)

                                                tCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatc                     <  1:2789595/71‑1 (MQ=255)

                                                 cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                      >  1:1064608/1‑69 (MQ=255)

                                                 cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                      >  1:3066812/1‑69 (MQ=255)

                                                 cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                      >  1:2633924/1‑69 (MQ=255)

                                                 cgcgCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                    >  1:3869491/1‑70 (MQ=255)

                                                  gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAgcg                                                    <  1:3232121/38‑1 (MQ=255)

                                                  gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctgc                                   >  1:2643547/1‑55 (MQ=255)

                                                  gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatca                    <  1:643106/70‑1 (MQ=255)

                                                  gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAg                   >  1:3474994/1‑71 (MQ=255)

                                                     ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                >  1:579528/1‑71 (MQ=255)

                                                      tGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt               >  1:3965443/1‑71 (MQ=255)

                                                       gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                 >  1:1403178/1‑36 (MQ=255)

                                                       gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                              >  1:895472/1‑39 (MQ=255)

                                                       gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt               >  1:2668514/1‑70 (MQ=255)

                                                       gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt               <  1:3796741/70‑1 (MQ=255)

                                                       gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt               <  1:2432739/70‑1 (MQ=255)

                                                         cGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                         >  1:2060623/1‑58 (MQ=255)

                                                         cGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc            <  1:2133907/71‑1 (MQ=255)

                                                          gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATg              <  1:2833312/68‑1 (MQ=255)

                                                          gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc            <  1:3300100/70‑1 (MQ=255)

                                                          gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc            <  1:2928519/70‑1 (MQ=255)

                                                          gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc            <  1:743027/70‑1 (MQ=255)

                                                           aaTATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc            <  1:1756823/69‑1 (MQ=255)

                                                            atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                  >  1:1036151/1‑46 (MQ=255)

                                                            atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt         <  1:226263/71‑1 (MQ=255)

                                                            atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt         >  1:949823/1‑71 (MQ=255)

                                                            atatGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGt         <  1:2747591/71‑1 (MQ=255)

                                                             tatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTg        <  1:3971231/71‑1 (MQ=255)

                                                               tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGa             >  1:1157143/1‑64 (MQ=255)

                                                               tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa       >  1:4068641/1‑70 (MQ=255)

                                                                gTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa       >  1:2343386/1‑69 (MQ=255)

                                                                 tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                <  1:133888/43‑1 (MQ=255)

                                                                 tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatca                    >  1:96637/1‑55 (MQ=255)

                                                                 tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt         <  1:492058/66‑1 (MQ=255)

                                                                   tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                  <  1:3571142/55‑1 (MQ=255)

                                                                   tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                >  1:805829/1‑57 (MQ=255)

                                                                   tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc   <  1:2776165/70‑1 (MQ=255)

                                                                    ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                >  1:2054602/1‑56 (MQ=255)

                                                                    ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatca  <  1:1885155/70‑1 (MQ=255)

TATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCA  >  minE/2677322‑2677459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`