BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,404	G→A	7.7%	L176L (CTG→TTG)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,404	0	G	A	7.7%	329.0 / 12.3	104	L176L (CTG→TTG)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base G (46/50); new base A (2/6); total (48/56)
Fisher's exact test for biased strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

Only 100 of 125 total aligned reads displayed.

ATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCG  >  minE/2677335‑2677474

ataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag                                                                        <  1:2213232/70‑1 (MQ=255)

   aGCAGGCCATTTGCTCTACCAGGCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcagcag                                                                     <  1:2688793/70‑1 (MQ=255)

     cAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                                  >  1:3481060/1‑71 (MQ=255)

             ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                                  >  1:2854845/1‑63 (MQ=255)

             ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                                          >  1:2458756/1‑71 (MQ=255)

              tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                                          <  1:780550/70‑1 (MQ=255)

              tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                                          <  1:785570/70‑1 (MQ=255)

               gCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                                          <  1:1143270/69‑1 (MQ=255)

               gCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                         <  1:1103915/70‑1 (MQ=255)

                ctctACCAGCCCCATCAGGTCGCGTTTACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGt                                                       <  1:1936557/71‑1 (MQ=255)

                     ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCcgcg                                                                   >  1:2626706/1‑54 (MQ=255)

                     ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                                   <  1:3055109/70‑1 (MQ=255)

                      cAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                                 <  1:1888385/71‑1 (MQ=255)

                       aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag                                                                        >  1:2314943/1‑47 (MQ=255)

                       aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                >  1:213696/1‑71 (MQ=255)

                       aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                <  1:2273773/71‑1 (MQ=255)

                        gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                >  1:686605/1‑70 (MQ=255)

                        atcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAt                                                <  1:2748717/68‑1 (MQ=255)

                          cccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAt                                             <  1:2768629/71‑1 (MQ=255)

                             aacAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAAtctc                                          <  1:867482/69‑1 (MQ=255)

                             aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                                  <  1:2636595/47‑1 (MQ=255)

                             aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                         >  1:718769/1‑56 (MQ=255)

                             aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGa                                              <  1:3594158/67‑1 (MQ=255)

                              tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                         <  1:405496/55‑1 (MQ=255)

                              tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                               <  1:2665079/65‑1 (MQ=255)

                                aGGTCGCGTTGATGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtt                                            <  1:1152073/66‑1 (MQ=255)

                                aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCagcagc                                                                    >  1:2718262/1‑42 (MQ=255)

                                aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                                >  1:3829174/1‑46 (MQ=255)

                                aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATa                                                              >  1:2398820/1‑48 (MQ=255)

                                aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATa                                                              >  1:3080168/1‑48 (MQ=255)

                                aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                           >  1:2140804/1‑51 (MQ=255)

                                aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                <  1:2743089/62‑1 (MQ=255)

                                aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                        >  1:3342403/1‑70 (MQ=255)

                                aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                        >  1:1449557/1‑70 (MQ=255)

                                aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                        >  1:2628654/1‑70 (MQ=255)

                                 ggTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCAt                                                               <  1:385073/46‑1 (MQ=255)

                                  gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                                  <  1:1615508/42‑1 (MQ=255)

                                  gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                                  <  1:1620294/42‑1 (MQ=255)

                                  atCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAta                                               <  1:1673469/60‑2 (MQ=255)

                                   tCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatc                                    <  1:2789595/71‑1 (MQ=255)

                                    cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                     >  1:1064608/1‑69 (MQ=255)

                                    cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                     >  1:3066812/1‑69 (MQ=255)

                                    cgcgCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                   >  1:3869491/1‑70 (MQ=255)

                                     gcgcTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                   <  1:649728/70‑2 (MQ=255)

                                     gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAgcg                                                                   <  1:3984979/38‑1 (MQ=255)

                                     gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAgcg                                                                   <  1:3232121/38‑1 (MQ=255)

                                     gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctgc                                                  >  1:2643547/1‑55 (MQ=255)

                                     gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatca                                   <  1:643106/70‑1 (MQ=255)

                                      cgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                 <  1:213280/71‑1 (MQ=255)

                                        ttGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                                  <  1:752886/36‑1 (MQ=255)

                                        ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                               >  1:579528/1‑71 (MQ=255)

                                         tGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                              >  1:3965443/1‑71 (MQ=255)

                                          gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                                >  1:1403178/1‑36 (MQ=255)

                                          gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                                             >  1:1795647/1‑39 (MQ=255)

                                          gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                                             >  1:895472/1‑39 (MQ=255)

                                          gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                              <  1:3796741/70‑1 (MQ=255)

                                          gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                              <  1:2432739/70‑1 (MQ=255)

                                          gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                              <  1:2946684/70‑1 (MQ=255)

                                            cGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                        >  1:2060623/1‑58 (MQ=255)

                                             gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                               >  1:515905/1‑66 (MQ=255)

                                             gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                           <  1:2928519/70‑1 (MQ=255)

                                             gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                           <  1:3300100/70‑1 (MQ=255)

                                              aaTATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                           <  1:1756823/69‑1 (MQ=255)

                                               atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                                 >  1:1036151/1‑46 (MQ=255)

                                               atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                        >  1:949823/1‑71 (MQ=255)

                                               atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                        <  1:1742926/71‑1 (MQ=255)

                                               atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                        <  1:226263/71‑1 (MQ=255)

                                               atatGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGt                        <  1:972013/71‑1 (MQ=255)

                                                tatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTg                       <  1:3971231/71‑1 (MQ=255)

                                                  tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGa                            >  1:1157143/1‑64 (MQ=255)

                                                  tGTTTTTGAATTAACTCCAGCAGCGACATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa                      >  1:253024/1‑70 (MQ=255)

                                                   gTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa                      >  1:2343386/1‑69 (MQ=255)

                                                    tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                               <  1:133888/43‑1 (MQ=255)

                                                    tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatca                                   >  1:96637/1‑55 (MQ=255)

                                                    tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                        <  1:492058/66‑1 (MQ=255)

                                                     ttctGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGTAATatc                  >  1:2935972/4‑71 (MQ=255)

                                                      tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                 <  1:3571142/55‑1 (MQ=255)

                                                      tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                               >  1:805829/1‑57 (MQ=255)

                                                      tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc                  <  1:3514089/70‑1 (MQ=255)

                                                      tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc                  <  1:2776165/70‑1 (MQ=255)

                                                       ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                               >  1:2054602/1‑56 (MQ=255)

                                                       ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatca                 <  1:1885155/70‑1 (MQ=255)

                                                        tGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaat               >  1:2162209/1‑71 (MQ=255)

                                                           aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg            <  1:2642815/71‑1 (MQ=255)

                                                           aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg            >  1:3365182/1‑71 (MQ=255)

                                                            ttAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                 <  1:305933/49‑1 (MQ=255)

                                                            ttAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGc           >  1:1876252/1‑71 (MQ=255)

                                                               aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa        <  1:1965513/71‑1 (MQ=255)

                                                               aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa        <  1:1013238/71‑1 (MQ=255)

                                                                 tCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                          <  1:2435502/35‑1 (MQ=255)

                                                                   cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                        <  1:92304/51‑1 (MQ=255)

                                                                   cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa        <  1:940192/67‑1 (MQ=255)

                                                                   cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa        <  1:886151/67‑1 (MQ=255)

                                                                    agcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGc   <  1:13675/71‑1 (MQ=255)

                                                                     gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg  >  1:1354640/1‑71 (MQ=255)

                                                                     gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg  <  1:1155648/71‑1 (MQ=255)

                                                                     gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg  <  1:2711612/71‑1 (MQ=255)

                                                                     gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg  >  1:1790572/1‑71 (MQ=255)

                                                                     gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg  >  1:591913/1‑71 (MQ=255)

                                                                     gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg  >  1:2598025/1‑71 (MQ=255)

ATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCG  >  minE/2677335‑2677474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`