BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,414	A→T	6.7%	R172R (CGT→CGA) ‡	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,414	0	A	T	6.7%	344.5 / 13.6	119	R172R (CGT→CGA) ‡	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base A (48/63); new base T (2/6); total (50/69)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 126 total aligned reads displayed.

TTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAA  >  minE/2677348‑2677483

ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATa                                                                       >  1:1678606/1‑67 (MQ=255)

ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                                                   >  1:2458756/1‑71 (MQ=255)

 tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                                                   <  1:780550/70‑1 (MQ=255)

 tGCTCTACAAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                                  >  1:2220854/1‑71 (MQ=255)

  gCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                                                   <  1:1143270/69‑1 (MQ=255)

  gCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                                  <  1:1103915/70‑1 (MQ=255)

   ctctACCAGCCCCATCAGGTCGCGTTTACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGt                                                                <  1:1936557/71‑1 (MQ=255)

        ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                                            <  1:3055109/70‑1 (MQ=255)

        ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                                            <  1:23658/70‑1 (MQ=255)

          aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                         >  1:213696/1‑71 (MQ=255)

          aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                         <  1:2273773/71‑1 (MQ=255)

          aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                         <  1:3037099/71‑1 (MQ=255)

           gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                         >  1:686605/1‑70 (MQ=255)

           atcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAt                                                         <  1:2748717/68‑1 (MQ=255)

            ccccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                                                      >  1:823735/1‑56 (MQ=255)

                aacAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAAtctc                                                   <  1:867482/69‑1 (MQ=255)

                aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGa                                                       <  1:3594158/67‑1 (MQ=255)

                 tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCgg                                                                 >  1:1112838/1‑56 (MQ=255)

                 tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                                  <  1:405496/55‑1 (MQ=255)

                 tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                                        <  1:2665079/65‑1 (MQ=255)

                 tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca                                                  >  1:3925326/1‑71 (MQ=255)

                   aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATa                                                                       >  1:2398820/1‑48 (MQ=255)

                   aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATa                                                                       >  1:3080168/1‑48 (MQ=255)

                   aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                         <  1:2743089/62‑1 (MQ=255)

                   aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                 >  1:2628654/1‑70 (MQ=255)

                   aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                 >  1:3342403/1‑70 (MQ=255)

                   aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                 >  1:1449557/1‑70 (MQ=255)

                     atCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAta                                                        <  1:1673469/60‑2 (MQ=255)

                      tCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatc                                             <  1:2789595/71‑1 (MQ=255)

                       cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                              >  1:1064608/1‑69 (MQ=255)

                       cgcgCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                            >  1:3869491/1‑70 (MQ=255)

                        gcgcTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                            <  1:649728/70‑2 (MQ=255)

                        gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctgc                                                           >  1:2643547/1‑55 (MQ=255)

                        gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatca                                            <  1:643106/70‑1 (MQ=255)

                         cgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                          <  1:213280/71‑1 (MQ=255)

                           ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                        >  1:579528/1‑71 (MQ=255)

                            tGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                       >  1:3965443/1‑71 (MQ=255)

                             gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                                                      >  1:895472/1‑39 (MQ=255)

                             gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                                                      >  1:1795647/1‑39 (MQ=255)

                             gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                       <  1:2432739/70‑1 (MQ=255)

                             gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                       <  1:3796741/70‑1 (MQ=255)

                             gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                       >  1:2668514/1‑70 (MQ=255)

                             gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                       <  1:2946684/70‑1 (MQ=255)

                               cGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                 >  1:2060623/1‑58 (MQ=255)

                               cGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                                    <  1:2133907/71‑1 (MQ=255)

                                gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                        >  1:515905/1‑66 (MQ=255)

                                gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATg                                      <  1:2833312/68‑1 (MQ=255)

                                gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                                    <  1:743027/70‑1 (MQ=255)

                                gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                                    <  1:2928519/70‑1 (MQ=255)

                                 aaTATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                                    <  1:1756823/69‑1 (MQ=255)

                                  atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                                          >  1:1036151/1‑46 (MQ=255)

                                  atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                 <  1:226263/71‑1 (MQ=255)

                                  atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                 <  1:1742926/71‑1 (MQ=255)

                                  atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                 >  1:949823/1‑71 (MQ=255)

                                  atatGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGt                                 <  1:972013/71‑1 (MQ=255)

                                  atatGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGt                                 <  1:2747591/71‑1 (MQ=255)

                                     tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGa                                     >  1:1157143/1‑64 (MQ=255)

                                     tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa                               >  1:4068641/1‑70 (MQ=255)

                                     tGTTTTTGAATTAACTCCAGCAGCGACATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa                               >  1:253024/1‑70 (MQ=255)

                                       tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                                        <  1:133888/43‑1 (MQ=255)

                                       tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatca                                            >  1:96637/1‑55 (MQ=255)

                                       tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                 <  1:492058/66‑1 (MQ=255)

                                         tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                          <  1:3571142/55‑1 (MQ=255)

                                         tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                        >  1:805829/1‑57 (MQ=255)

                                         tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc                           <  1:3514089/70‑1 (MQ=255)

                                         tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc                           <  1:2776165/70‑1 (MQ=255)

                                          ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatca                          <  1:1885155/70‑1 (MQ=255)

                                           tGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaat                        >  1:2162209/1‑71 (MQ=255)

                                              aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                     >  1:3365182/1‑71 (MQ=255)

                                              aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                     <  1:2642815/71‑1 (MQ=255)

                                               ttAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                          <  1:305933/49‑1 (MQ=255)

                                               ttAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGc                    >  1:1876252/1‑71 (MQ=255)

                                                  aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                 <  1:1013238/71‑1 (MQ=255)

                                                  aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                 <  1:1965513/71‑1 (MQ=255)

                                                  aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                 <  1:1950184/71‑1 (MQ=255)

                                                      cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                 <  1:642744/51‑1 (MQ=255)

                                                      cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                 <  1:92304/51‑1 (MQ=255)

                                                      cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                 <  1:886151/67‑1 (MQ=255)

                                                      cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAAt              <  1:1845628/70‑1 (MQ=255)

                                                      cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAAt              <  1:1429735/70‑1 (MQ=255)

                                                       agcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGc            <  1:13675/71‑1 (MQ=255)

                                                        gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg           >  1:927319/1‑71 (MQ=255)

                                                        gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg           <  1:1155648/71‑1 (MQ=255)

                                                        gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg           >  1:2598025/1‑71 (MQ=255)

                                                          agcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGa         <  1:1081708/71‑1 (MQ=255)

                                                           gcgcCATACGTCGGTGCTGCATGATTTAATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAg        >  1:3446834/1‑71 (MQ=255)

                                                            cgcCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAgg       <  1:1393371/71‑1 (MQ=255)

                                                             gcCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAgg       >  1:3236335/1‑70 (MQ=255)

                                                               cATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTg                                <  1:4143273/43‑1 (MQ=255)

                                                               cATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCa     >  1:524081/1‑70 (MQ=255)

                                                                aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatca                      <  1:1130800/52‑1 (MQ=255)

                                                                aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCATTCAGCGGAAATGCGGAGGCATa   <  1:3300270/71‑1 (MQ=255)

                                                                aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg           <  1:1348536/63‑1 (MQ=255)

                                                                aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCAt    >  1:3647826/1‑70 (MQ=255)

                                                                aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCAt    >  1:3073373/1‑70 (MQ=255)

                                                                 tACGTCGGTGCTGCATGATTTCATCATCAGGGATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaa  <  1:753398/71‑1 (MQ=255)

                                                                 tACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAg        <  1:757004/65‑1 (MQ=255)

                                                                  aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGaaa               >  1:794964/1‑57 (MQ=255)

                                                                  aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGc            <  1:3208772/60‑1 (MQ=255)

                                                                  aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCa     <  1:23824/67‑1 (MQ=255)

TTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAA  >  minE/2677348‑2677483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`