BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,415	C→T	6.6%	R172H (CGT→CAT) ‡	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,415	0	C	T	6.6%	393.9 / 11.3	120	R172H (CGT→CAT) ‡	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base C (46/66); new base T (2/6); total (48/72)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAG  >  minE/2677348‑2677485

ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                                                     >  1:2458756/1‑71 (MQ=255)

 tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                                                     <  1:780550/70‑1 (MQ=255)

 tGCTCTACAAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                                    >  1:2220854/1‑71 (MQ=255)

  gCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                                    <  1:1103915/70‑1 (MQ=255)

   ctctACCAGCCCCATCAGGTCGCGTTTACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGt                                                                  <  1:1936557/71‑1 (MQ=255)

        ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                                              <  1:23658/70‑1 (MQ=255)

         cAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                                            <  1:1888385/71‑1 (MQ=255)

          aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                           >  1:213696/1‑71 (MQ=255)

          aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                           <  1:2273773/71‑1 (MQ=255)

          aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                           <  1:3037099/71‑1 (MQ=255)

           gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                           >  1:686605/1‑70 (MQ=255)

            ccccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                                                        >  1:823735/1‑56 (MQ=255)

             cccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAt                                                        <  1:2768629/71‑1 (MQ=255)

                aacAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAAtctc                                                     <  1:867482/69‑1 (MQ=255)

                aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                                    >  1:718769/1‑56 (MQ=255)

                aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGa                                                         <  1:3594158/67‑1 (MQ=255)

                 tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCgg                                                                   >  1:1112838/1‑56 (MQ=255)

                 tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                                    <  1:405496/55‑1 (MQ=255)

                 tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca                                                    >  1:3925326/1‑71 (MQ=255)

                   aGGTCGCGTTGATGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtt                                                       <  1:1152073/66‑1 (MQ=255)

                   aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                                      >  1:2140804/1‑51 (MQ=255)

                   aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                           <  1:2743089/62‑1 (MQ=255)

                   aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                   >  1:1449557/1‑70 (MQ=255)

                   aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                   >  1:2628654/1‑70 (MQ=255)

                     atCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAta                                                          <  1:1673469/60‑2 (MQ=255)

                      tCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatc                                               <  1:2789595/71‑1 (MQ=255)

                       cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                                >  1:2633924/1‑69 (MQ=255)

                       cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                                >  1:1064608/1‑69 (MQ=255)

                       cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                                >  1:3066812/1‑69 (MQ=255)

                        gcgcTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                              <  1:649728/70‑2 (MQ=255)

                        gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctgc                                                             >  1:2643547/1‑55 (MQ=255)

                        gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatca                                              <  1:643106/70‑1 (MQ=255)

                        gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAg                                             >  1:3474994/1‑71 (MQ=255)

                         cgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                            <  1:213280/71‑1 (MQ=255)

                           ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                          >  1:579528/1‑71 (MQ=255)

                            tGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                         >  1:3965443/1‑71 (MQ=255)

                             gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                                                        >  1:1795647/1‑39 (MQ=255)

                             gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                                                        >  1:895472/1‑39 (MQ=255)

                             gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                         <  1:3796741/70‑1 (MQ=255)

                             gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                         >  1:2668514/1‑70 (MQ=255)

                             gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                         <  1:2946684/70‑1 (MQ=255)

                               cGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                   >  1:2060623/1‑58 (MQ=255)

                                gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                          >  1:515905/1‑66 (MQ=255)

                                gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                                      <  1:2928519/70‑1 (MQ=255)

                                gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                                      <  1:3300100/70‑1 (MQ=255)

                                  atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                                            >  1:1036151/1‑46 (MQ=255)

                                  atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                   <  1:1742926/71‑1 (MQ=255)

                                  atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                   >  1:949823/1‑71 (MQ=255)

                                  atatGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGt                                   <  1:2747591/71‑1 (MQ=255)

                                  atatGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGt                                   <  1:972013/71‑1 (MQ=255)

                                   tatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTg                                  <  1:3971231/71‑1 (MQ=255)

                                     tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGa                                       >  1:1157143/1‑64 (MQ=255)

                                     tGTTTTTGAATTAACTCCAGCAGCGACATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa                                 >  1:253024/1‑70 (MQ=255)

                                      gTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa                                 >  1:2343386/1‑69 (MQ=255)

                                       tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                                          <  1:133888/43‑1 (MQ=255)

                                       tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatca                                              >  1:96637/1‑55 (MQ=255)

                                        ttctGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGTAATatc                             >  1:2935972/4‑71 (MQ=255)

                                         tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                          >  1:805829/1‑57 (MQ=255)

                                         tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc                             <  1:3514089/70‑1 (MQ=255)

                                         tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc                             <  1:2776165/70‑1 (MQ=255)

                                          ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                          >  1:2054602/1‑56 (MQ=255)

                                          ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatca                            <  1:1885155/70‑1 (MQ=255)

                                           tGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaat                          >  1:2162209/1‑71 (MQ=255)

                                              aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                       >  1:3365182/1‑71 (MQ=255)

                                              aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                       <  1:2642815/71‑1 (MQ=255)

                                              aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                       >  1:3741100/1‑71 (MQ=255)

                                               ttAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                            <  1:305933/49‑1 (MQ=255)

                                                  aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                   <  1:1965513/71‑1 (MQ=255)

                                                  aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                   <  1:1013238/71‑1 (MQ=255)

                                                    tCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                                     <  1:2435502/35‑1 (MQ=255)

                                                      cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                   <  1:92304/51‑1 (MQ=255)

                                                      cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                   <  1:886151/67‑1 (MQ=255)

                                                      cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAAt                <  1:1845628/70‑1 (MQ=255)

                                                       agcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGc              <  1:13675/71‑1 (MQ=255)

                                                        gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg             <  1:2711612/71‑1 (MQ=255)

                                                        gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg             <  1:1155648/71‑1 (MQ=255)

                                                        gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg             >  1:1354640/1‑71 (MQ=255)

                                                        gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg             >  1:1790572/1‑71 (MQ=255)

                                                        gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg             >  1:591913/1‑71 (MQ=255)

                                                        gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg             >  1:927319/1‑71 (MQ=255)

                                                           gcgcCATACGTCGGTGCTGCATGATTTAATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAg          >  1:3446834/1‑71 (MQ=255)

                                                            cgcCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAgg         <  1:1393371/71‑1 (MQ=255)

                                                             gcCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAgg         >  1:3236335/1‑70 (MQ=255)

                                                               cATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTg                                  <  1:4143273/43‑1 (MQ=255)

                                                               cATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCa       >  1:524081/1‑70 (MQ=255)

                                                                aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatca                        <  1:1130800/52‑1 (MQ=255)

                                                                aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatca                        <  1:109437/52‑1 (MQ=255)

                                                                aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCATTCAGCGGAAATGCGGAGGCATa     <  1:3300270/71‑1 (MQ=255)

                                                                aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCAt      >  1:1469550/1‑70 (MQ=255)

                                                                aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCAt      >  1:3073373/1‑70 (MQ=255)

                                                                aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCAt      >  1:3647826/1‑70 (MQ=255)

                                                                 tACGTCGGTGCTGCATGATTTCATCATCAGGGATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaa    <  1:753398/71‑1 (MQ=255)

                                                                 tACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAg          <  1:757004/65‑1 (MQ=255)

                                                                  aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGaaa                 >  1:794964/1‑57 (MQ=255)

                                                                  aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGc              <  1:3208772/60‑1 (MQ=255)

                                                                  aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg             <  1:465720/61‑1 (MQ=255)

                                                                  aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCa       <  1:23824/67‑1 (MQ=255)

                                                                   cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaat                          >  1:1810413/1‑47 (MQ=255)

                                                                   cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGc        >  1:424480/1‑65 (MQ=255)

                                                                   cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAg  >  1:1005280/1‑71 (MQ=255)

TTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAG  >  minE/2677348‑2677485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`